Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Elongation factor 2

Gene

fusA

Organism
Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 34GTPBy similarity8
Nucleotide bindingi93 – 97GTPBy similarity5
Nucleotide bindingi147 – 150GTPBy similarity4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 2
Short name:
EF-2
Gene namesi
Name:fusA
Synonyms:fus
Ordered Locus Names:Saci_0603
OrganismiSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Taxonomic identifieri330779 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001018 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000910472 – 737Elongation factor 2Add BLAST736

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei604DiphthamideBy similarity1

Interactioni

Protein-protein interaction databases

STRINGi330779.Saci_0603.

Structurei

3D structure databases

ProteinModelPortaliP23112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 262tr-type GAdd BLAST245

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01559. Archaea.
COG0480. LUCA.
HOGENOMiHOG000231589.
KOiK03234.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRYKTVEQV LSLMKDVTRV RNIGIIAHVD HGKTTTSDTL LAASGIISQK
60 70 80 90 100
VAGEALALDY LSVEQQRGIT VKAANISLYH EIDGKGYVIN LIDTPGHVDF
110 120 130 140 150
SGRVTRSLRV LDGSIVVIDA VEGIMTQTET VLRQSLEERV RPILFINKVD
160 170 180 190 200
RLIKELKLSS QEIQKRLIDL IIEVNNLIET YGEPEFKDQW KIKPELGNVV
210 220 230 240 250
FGSAKDKWGF SVPMAGKRGV KFSDVVNAYT SGDKAKIEEL ASKVPIHEAL
260 270 280 290 300
LDAVIKFVPN PRDSQKYRIP KIWKGDLDSE IAKAMINADP NGPIVMMIND
310 320 330 340 350
MKVDPHAGLV ATGRVFSGTL RAGEEVWLVN AKRQQRILQV SLYMGAIREL
360 370 380 390 400
AEEIPVGNIA AALGMDAARS GETGVDIRFK DSVLGSFEKL HYISEPVVTI
410 420 430 440 450
SVEPRNPKDL TKMIDALRKL SIEDSNLVVK INEETGEYLL SGMGFLHLEV
460 470 480 490 500
SLQLLKENYG LDVVTTPPIV VYRESIRNKS QVFEGKSPNK HNKLYISVEP
510 520 530 540 550
LNNQTIDLIA NGTIKEDMDN KEMAKILRDQ AEWDYDEAKK IVAIDENINV
560 570 580 590 600
FIDATSGVQH LREIMDTLLQ GFRLAMKEGP LAFEPVRGVK VVLHDAVVHE
610 620 630 640 650
DPAHRGPAQL YPAVRNAIFA GILTSKPTLL EPLQKLDIRI PMEYLGNVTA
660 670 680 690 700
VITRKRGKVI NVVQTGNVAR VYAEIPVGES FELASELRAS SAGRAFWGTE
710 720 730
FSRWAPVPDS ILVDLIMKIR ERKGKPKQLP KVEDFIS
Length:737
Mass (Da):81,978
Last modified:January 23, 2007 - v3
Checksum:iF3D2E2D7FE4077A7
GO

Sequence cautioni

The sequence AAY79995 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138E → G in CAA38716 (PubMed:1900048).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54972 Genomic DNA. Translation: CAA38716.1.
CP000077 Genomic DNA. Translation: AAY79995.1. Different initiation.
PIRiS14408.
RefSeqiWP_015385460.1. NC_007181.1.

Genome annotation databases

EnsemblBacteriaiAAY79995; AAY79995; Saci_0603.
GeneIDi3473165.
KEGGisai:Saci_0603.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54972 Genomic DNA. Translation: CAA38716.1.
CP000077 Genomic DNA. Translation: AAY79995.1. Different initiation.
PIRiS14408.
RefSeqiWP_015385460.1. NC_007181.1.

3D structure databases

ProteinModelPortaliP23112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi330779.Saci_0603.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAY79995; AAY79995; Saci_0603.
GeneIDi3473165.
KEGGisai:Saci_0603.

Phylogenomic databases

eggNOGiarCOG01559. Archaea.
COG0480. LUCA.
HOGENOMiHOG000231589.
KOiK03234.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00054_A. EF_G_EF_2_A. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004543. Transl_elong_EFG/EF2_arc.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00490. aEF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEF2_SULAC
AccessioniPrimary (citable) accession number: P23112
Secondary accession number(s): Q4JB34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.