P23110 (2SS8_HELAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Albumin-8 Alternative name(s): Methionine-rich 2S protein SFA8
Organism	Helianthus annuus (Common sunflower)
Taxonomic identifier	4232 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Heliantheae alliance › Heliantheae › Helianthus

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is a 2S seed storage protein.
Subunit structure	Heterodimer; disulfide-linked.
Sequence similarities	Belongs to the 2S seed storage albumins family.

Ontologies

Keywords
Domain	Signal
Molecular function	Seed storage protein Storage protein
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular function	nutrient reservoir activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Propeptide

26 – 38

13

PRO_0000032153

Chain

39 – 141

103

Albumin-8

PRO_0000032154

Amino acid modifications

Disulfide bond

49 ↔ 100

Disulfide bond

62 ↔ 89

Disulfide bond

90 ↔ 132

Disulfide bond

102 ↔ 139

Experimental info

Sequence conflict

67

1

M → N AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

141

Helix Strand Turn

Details...

Beta strand

46 – 48

3

Helix

49 – 55

7

Helix

61 – 66

6

Turn

67 – 70

4

Beta strand

77 – 79

3

Helix

87 – 95

9

Helix

98 – 100

3

Helix

103 – 108

6

Helix

118 – 131

14

Beta strand

134 – 137

4

Sequences

	Sequence		Length	Mass (Da)
	P23110 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 1E5723B9122C9BD4	FASTA	141	16,090
	10 20 30 40 50 60 MARFSIVFAA AGVLLLVAMA PVSEASTTTI ITTIIEENPY GRGRTESGCY QQMEEAEMLN 70 80 90 100 110 120 HCGMYLMKNL GERSQVSPRM REEDHKQLCC MQLKNLDEKC MCPAIMMMLN EPMWIRMRDQ 130 140 VMSMAHNLPI ECNLMSQPCQ M

References

[1]	"Amino acid and cDNA sequences of a methionine-rich 2S protein from sunflower seed (Helianthus annuus L.)." Kortt A.A., Caldwell J.B., Lilley G.G., Higgins T.J.V. Eur. J. Biochem. 195:329-334(1991) [PubMed: 1997318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 39-141. Tissue: Seed.
[2]	"Disulphide structure of a sunflower seed albumin: conserved and variant disulphide bonds in the cereal prolamin superfamily." Egorov T.A., Odintsova T.I., Musolyamov A.K., Fido R., Tatham A.S., Shewry P.R. FEBS Lett. 396:285-288(1996) [PubMed: 8915004] [Abstract] Cited for: PROTEIN SEQUENCE OF 39-141. Strain: cv. Hybrid 246. Tissue: Seed.
[3]	"Solution structure of a methionine-rich 2S albumin from sunflower seeds: relationship to its allergenic and emulsifying properties." Pantoja-Uceda D., Shewry P.R., Bruix M., Tatham A.S., Santoro J., Rico M. Biochemistry 43:6976-6986(2004) [PubMed: 15170335] [Abstract] Cited for: STRUCTURE BY NMR OF 39-141, DISULFID BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X56686 mRNA. Translation: CAA40015.1.

PIR

S14259.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1S6D	NMR	-	A	39-141	[»]

ProteinModelPortal

P23110.

SMR

P23110. Positions 39-141.

ModBase

Search...

Protein family/group databases

Allergome

772. Hel a 2S Albumin.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR016140. Bifunc_inhib/LTP/seed_store.
IPR003612. LTP/seed_store/tryp_amyl_inhib.
IPR013771. Trypsin/amylase_inhib.
[Graphical view]

Gene3D

G3DSA:1.10.120.10. Trypsin/amylase_inhib. 1 hit.

Pfam

PF00234. Tryp_alpha_amyl. 1 hit.
[Graphical view]

SMART

SM00499. AAI. 1 hit.
[Graphical view]

SUPFAM

SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.

ProtoNet

Search...

Entry information

Entry name

2SS8_HELAN

Accession

Primary (citable) accession number: P23110

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	January 11, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program