P23106 (XYLF_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-hydroxymuconate semialdehyde hydrolase
Short name=HMSH
EC=3.7.1.9

Alternative name(s):
2-hydroxymuconic semialdehyde hydrolase

Gene names

Name:

xylF

Encoded on

Plasmid TOL pWW0

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of 2-hydroxymuconate semialdehyde to 2-hydroxypent-2,4-dienoate. Ref.2
Catalytic activity	2-hydroxymuconate semialdehyde + H2O = formate + 2-oxopent-4-enoate. Ref.2
Pathway	Aromatic compound metabolism; benzoate degradation via hydroxylation.
Sequence similarities	Belongs to the DmpD/TodF/XylF esterase family.
Biophysicochemical properties	Kinetic parameters: KM=30 µM for catechol Ref.2 KM=36 µM for 3-methylcatechol KM=10 µM for 4-methylcatechol KM=15 µM for 3-ethylcatechol KM=22 µM for 4-ethylcatechol KM=5 µM for 3-propylcatechol KM=23 µM for 4-propylcatechol KM=37 µM for 3-allylcatechol pH dependence: Optimum pH is 7-7.5.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Hydrolase Serine esterase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological_process	benzoate catabolic process via hydroxylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	2-hydroxymuconate-semialdehyde hydrolase activity Inferred from electronic annotation. Source: EC carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	2-hydroxymuconate semialdehyde hydrolase		PRO_0000207058
Sites
	Active site	106	1	By similarity
	Active site	227	1	By similarity
	Active site	255	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23106 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: ED8D215E6DD47DF5	FASTA	281	30,598
	10 20 30 40 50 60 MNAPQQSPEI GREILAAGYR TNLHDQGEGF PALLIHGSGP ASPPGPTGAG SFRSSQTRRV 70 80 90 100 110 120 IAPDMLGFGY SERPADGKYS QARWVEHAIG VLDALGIQQG DIVGNSFGGG LALALAIRHP 130 140 150 160 170 180 ERVRRLVLMG SVGVSFPITA GLETAWGYTP SLANMRRLLD LFAHDRTLVN DELAELRYQA 190 200 210 220 230 240 SIRPGFQESF AAMFPPPRQN GVDDLASNET DIRALPNETL VIHGREDRII PLQASLTLAQ 250 260 270 280 WIPNAQLHVF GQCGHWTQIE HAERFARLVE NFLAEADALH S

References

[1]	"DNA sequence determination of the TOL plasmid (pWWO) xylGFJ genes of Pseudomonas putida: implications for the evolution of aromatic catabolism." Horn J.M., Harayama S., Timmis K.N. Mol. Microbiol. 5:2459-2474(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23.
[2]	"Purification and some properties of the 2-hydroxy-6-oxohepta-2,4-dienoate hydrolase (2-hydroxymuconic semialdehyde hydrolase) encoded by the TOL plasmid pWW0 from Pseudomonas putida mt-2." Duggleby C.J., Williams P.A. J. Gen. Microbiol. 132:2459-2474(1991) Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64747 Genomic DNA. Translation: AAA26054.1.
PIR	S18245.
RefSeq	NP_542864.1. NC_003350.1.
3D structure databases
ProteinModelPortal	P23106.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1218747.
Phylogenomic databases
ProtClustDB	CLSK2754119.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-12763.
UniPathway	UPA00156.
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. [Graphical view]
Pfam	PF00561. Abhydrolase_1. 1 hit. [Graphical view]
PRINTS	PR00111. ABHYDROLASE.
ProtoNet	Search...

Entry information

Entry name

XYLF_PSEPU

Accession

Primary (citable) accession number: P23106

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program