P23105 (XYLG_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-hydroxymuconic semialdehyde dehydrogenase
Short name=HMSD
EC=1.2.1.-

Gene names

Name:

xylG

Encoded on

Plasmid TOL pWW0

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	2-hydroxymuconic acid semialdehyde can be converted to 2-hydroxypent-2,4-dienoate either directly by the action of 2-hydroxymuconic semialdehyde hydrolase (HMSH) or by the action of three sequential enzymes, the first of which is HMSD. Can oxidize not only 2-hydroxymuconic semialdehyde and its analogs but also benzaldehyde and its analogs.
Pathway	Aromatic compound metabolism; benzoate degradation via hydroxylation.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.3 for the oxidation of 2-hydroxymuconic semialdehyde, and 9.6 for that of benzaldehyde.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	2-hydroxymuconic semialdehyde dehydrogenase		PRO_0000056594
Sites
	Active site	254	1	By similarity
	Active site	288	1	By similarity
Experimental info
	Sequence conflict	11 – 12	2	EL → AF AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P23105 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 854303F121B2FFBD	FASTA	486	51,761
	10 20 30 40 50 60 MKEIKHFISG ELVGSASGKL FDNVSPANGQ VIGRVHEAGR AEVDAAVRAA RAALKGPWGK 70 80 90 100 110 120 MTVAERAEIL HRVADGITAR FGEFLEARMP GHRQAEVAGQ PHRHSARRAN FKVFADLLKN 130 140 150 160 170 180 VANEAFEMAT PDGAGALNYG VRRPKGVIGV ISPWNLPLLL MTWKVGPALA CGNCVVVKPS 190 200 210 220 230 240 EETPLTATLL GEVMQAAGVP AGVYNVVHGF GGDSAGAFLT EHPDVDAYTF TGETGTGETI 250 260 270 280 290 300 MRAAAKGVRQ VSLELGGKNA GIVFADCDMD KAIEGTLRSA FANCGQVCLG TERVYVERPI 310 320 330 340 350 360 FDAFVARLKA GAEALKIGEP NDPEANFGPL ISHKPREKVP SYYQQAVDDG ATVVTGGGVP 370 380 390 400 410 420 EMPAHLAGGA WVQPTIWTGL ADDSAVVTEE IFGPCCHIRP FDSEEEAIEL ANSLPYGLAS 430 440 450 460 470 480 AIWTENVRRA HRVAGQIEAG IVWVNSWFLR DLRTAFGGSK QSGIGREGGV HSLEFYTELK NICVKL

References

[1]	"DNA sequence determination of the TOL plasmid (pWWO) xylGFJ genes of Pseudomonas putida: implications for the evolution of aromatic catabolism." Horn J.M., Harayama S., Timmis K.N. Mol. Microbiol. 5:2459-2474(1991) [PubMed: 1791759] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase." Steele M.I., Lorenz D., Hatter K., Park A., Sokatch J.R. J. Biol. Chem. 267:13585-13592(1992) [PubMed: 1339433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PAO.
[3]	"Overlapping substrate specificities of benzaldehyde dehydrogenase (the xylC gene product) and 2-hydroxymuconic semialdehyde dehydrogenase (the xylG gene product) encoded by TOL plasmid pWW0 of Pseudomonas putida." Inoue J., Shaw J.P., Rekik M., Harayama S. J. Bacteriol. 177:1196-1201(1995) [PubMed: 7868591] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-15.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64747 Genomic DNA. Translation: AAA26053.1.
PIR	E42902.
RefSeq	NP_542865.1. NC_003350.1.
3D structure databases
ProteinModelPortal	P23105.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1218748.
Phylogenomic databases
ProtClustDB	CLSK862335.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-3402.
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR017628. OHmuconic_semiald_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR03216. OH_muco_semi_DH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLG_PSEPU

Accession

Primary (citable) accession number: P23105

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	September 21, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program