Reviewed, UniProtKB/Swiss-Prot P23102 (XYLL_PSEPU)

Names and origin

Protein names

Recommended name:
    1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase
    EC=1.3.1.25
Alternative name(s):
    Cis-1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase
    Cis-1,2-dihydroxy-3,4-cyclohexadiene-1-carboxylate dehydrogenase
    2-hydro-1,2-dihydroxybenzoate dehydrogenase
      Short name=DHB dehydrogenase

Gene names

Name:

xylL

Encoded on

Plasmid TOL pWW0

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degradation of 2-hydro-1,2-dihydroxy benzoate (DHB) to catechol.
Catalytic activity	(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH.
Pathway	Aromatic compound metabolism; benzoic acid degradation via hydroxylation; catechol from benzoic acid: step 2/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase		PRO_0000054814
Regions
	Nucleotide binding	11 – 35	25	NAD By similarity
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	142	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23102-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 23708B9757C6FC41	FASTA	269	28,883
	10 20 30 40 50 60 MNKRFQGKVA VITGAAQGIG RRVAERMAAE GGRLLLVDRS ELIHELADEL VGVAEVLTLT 70 80 90 100 110 120 ADLEQFAECQ RVMAAALERF GRLDILINNV GGTIWAKPFE HYQEREIEAE VRRSLFPTLW 130 140 150 160 170 180 CCHAALAPMI EQGSGAIVNV SSVATRGIHR VPYGAAKGGV NALTACLAFE TAERGIRVNA 190 200 210 220 230 240 TAPGGTEARH GGFRNSAEPS EQEKVWYQQI VDQSLDSSLM KRYGSIDEQV EAILFLASDA 250 260 ASYITGITLP VAGGDLGCQS CSVMFSVSG

References

[1]

"Cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily." Neidle E.L., Hartnett C., Ornston L.N., Bairoch A., Rekik M., Harayama S. Eur. J. Biochem. 204:113-120(1992) [PubMed: 1740120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M64747 Genomic DNA. Translation: AAA26050.1.
PIR	S23485.
RefSeq	NP_542868.1.
3D structure databases
HSSP	HSSP built from PDB template 1IY8 based on UniProtKB Q9LBG2.
ModBase	Search...
Genome annotation databases
GeneID	1218753.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-2964.
BRENDA	1.3.1.25. 403.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLL_PSEPU

Accession

Primary (citable) accession number: P23102

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)