P23101 (XYLZ_PSEPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Toluate 1,2-dioxygenase electron transfer component

Including the following 2 domains:

1. Ferredoxin
2. Ferredoxin--NAD(+) reductase
   EC=1.18.1.3

Gene names

Name:

xylZ

Encoded on

Plasmid TOL pWW0

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Electron transfer component of toluate 1,2-dioxygenase system.
Catalytic activity	Reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH.
Cofactor	FAD By similarity. Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	This dioxygenase system consists of three proteins: the two subunits of the hydroxylase component (xylX and xylY), and an electron transfer component (xylZ).
Sequence similarities	Belongs to the bacterial ring-hydroxylating dioxygenase ferredoxin reductase family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	2Fe-2S FAD Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-NAD+ reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Toluate 1,2-dioxygenase electron transfer component		PRO_0000167659
Regions
	Domain	3 – 97	95	2Fe-2S ferredoxin-type
	Domain	104 – 204	101	FAD-binding FR-type
	Region	99 – 336	238	Ferredoxin-reductase
Sites
	Metal binding	40	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	45	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	48	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	81	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23101 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 2E74B6A2E2757CAB	FASTA	336	36,220
	10 20 30 40 50 60 MTHKVATDFE DGVTRFIDAN TGETVADAAY RQGINLPLDC RDGACGACKC FAESGRYSLG 70 80 90 100 110 120 EEYIEDALSE AEAEQGYVLT CQMRAESDCV IRVPAASDVC KTQQAGYQAA ISNVRQLSES 130 140 150 160 170 180 TIALSIKSAS LNQLAFLPGQ YVNLQVPGSD QTRAYSFSSL QKDGEVSFLI RKLPGGLMSS 190 200 210 220 230 240 FLTSLAKVGD SVSLAGPLGA FYLREIKRPL LLLAGGTGLA PFTAMLEKIA EQGGEHPLHL 250 260 270 280 290 300 IYGVTHDHDL VEMDKLEAFA ARIPNFSYSA CVASPDSAYP QKGYVTQYIE PKQLNGGEVD 310 320 330 IYLCGPPPMV EAVSQYIRAQ GIQPANFYYE KFAASA

References

[1]	"Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas putida TOL pWW0 plasmid xylXYZ, genes encoding benzoate dioxygenases." Harayama S., Rekik M., Bairoch A., Neidle E.L., Ornston L.N. J. Bacteriol. 173:7540-7548(1991) [PubMed: 1938949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64747 Genomic DNA. Translation: AAA26049.1.
PIR	C41659.
RefSeq	NP_542869.1. NC_003350.1.
3D structure databases
ProteinModelPortal	P23101.
SMR	P23101. Positions 2-335.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1218764.
Phylogenomic databases
ProtClustDB	CLSK867330.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-2963.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR012675. Beta-grasp_ferredoxin-type. IPR017927. Fd_Rdtase_FAD-bd. IPR001041. Ferredoxin. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR001221. Phe_hydroxylase. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00111. Fer2. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00371. FPNCR. PR00410. PHEHYDRXLASE.
SUPFAM	SSF54292. Ferredoxin. 1 hit. SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLZ_PSEPU

Accession

Primary (citable) accession number: P23101

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program