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P23090

- GAG_MLVDU

UniProt

P23090 - GAG_MLVDU

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Protein

Gag polyprotein

Gene

gag

Organism
Duplan murine leukemia virus
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).By similarity
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei129 – 1302Cleavage; by viral protease p14By similarity
Sitei206 – 2072Cleavage; by viral protease p14By similarity
Sitei469 – 4702Cleavage; by viral protease p14By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri493 – 51018CCHC-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. virion assembly Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Nucleocapsid protein p10
Short name:
NC-gag
Gene namesi
Name:gag
OrganismiDuplan murine leukemia virus
Taxonomic identifieri11794 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]

Subcellular locationi

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
  3. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by hostBy similarity
Chaini2 – 529528Gag polyproteinBy similarityPRO_0000390810Add
BLAST
Chaini2 – 129128Matrix protein p15Sequence AnalysisPRO_0000040892Add
BLAST
Chaini130 – 20677RNA-binding phosphoprotein p12Sequence AnalysisPRO_0000040893Add
BLAST
Chaini207 – 469263Capsid protein p30Sequence AnalysisPRO_0000040894Add
BLAST
Chaini470 – 52960Nucleocapsid protein p10Sequence AnalysisPRO_0000040895Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by hostBy similarity

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP23090.
SMRiP23090. Positions 2-98, 207-338, 343-373, 470-525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili429 – 46739Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi176 – 1794PPXY motifSequence Analysis

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Gag-p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase (Potential).Curated

Sequence similaritiesi

Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri493 – 51018CCHC-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23090 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGQTVTTPLS LTLDHWKDVQ CIASNQSVDV KRRRWVTFCS VEWPSFDVGW
60 70 80 90 100
PLDGTFNLDI ILQVKSKVTC PGPHGHPDQV PYIVTWEALV YHPPPWVKPF
110 120 130 140 150
VSPKPFPLST LPFSPPGPSA HPPSRSDLYT ALIPSKPPKS RVLPPNGGPL
160 170 180 190 200
IDLLTENLPN LPPLSKGPVK KRRPPPPRYS PPNPMESRVR GRRDPPAADS
210 220 230 240 250
TSSQAFPLRM GGDGQLQYWP FSSSDLYNWK NNNPSFSEDP GKLTALIESV
260 270 280 290 300
LTTHQPTWDD CQQLLGTLLT GEEKQRVLLE ARKAVRGNDG RPTQLPNEVN
310 320 330 340 350
SAFPLERPDW NYSTPEGRNH LVLYRQLLLA GLHNAGRSPT NLAKVKRITQ
360 370 380 390 400
GPNESPSAFL ERLKEAYRRY TPYDPEDPGQ ETNVSMSFIW QSAPDIGRKL
410 420 430 440 450
ERLEDLKSKT LGDLVREAEK IFNKRETPEE REERIRRETE EKEERRRAED
460 470 480 490 500
EQREKERDRR RHREMSKFLA TVVTGQRQDR QGGERRRPQL DEDQCAYCKE
510 520
KGHWAKDCPK KPRGPRGPRP QTSLLTLGD
Length:529
Mass (Da):60,318
Last modified:January 23, 2007 - v3
Checksum:i4B0677A58AADDD15
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14576 Genomic DNA. Translation: CAA32715.1.
PIRiS03901. FOMVDU.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14576 Genomic DNA. Translation: CAA32715.1 .
PIRi S03901. FOMVDU.

3D structure databases

ProteinModelPortali P23090.
SMRi P23090. Positions 2-98, 207-338, 343-373, 470-525.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProi IPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view ]
SMARTi SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEi PS50158. ZF_CCHC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Severe immunodeficiency disease induced by a defective murine leukaemia virus."
    Aziz D.C., Hanna Z., Jolicoeur P.
    Nature 338:505-508(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGAG_MLVDU
AccessioniPrimary (citable) accession number: P23090
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3