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Protein

Gag polyprotein

Gene

gag

Organism
Duplan murine leukemia virus
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).By similarity
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri493 – 510CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Viral nucleoprotein
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Nucleocapsid protein p10
Short name:
NC-gag
Gene namesi
Name:gag
OrganismiDuplan murine leukemia virus
Taxonomic identifieri11794 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
ChainiPRO_00003908102 – 529Gag polyproteinBy similarityAdd BLAST528
ChainiPRO_00000408922 – 129Matrix protein p15Sequence analysisAdd BLAST128
ChainiPRO_0000040893130 – 206RNA-binding phosphoprotein p12Sequence analysisAdd BLAST77
ChainiPRO_0000040894207 – 469Capsid protein p30Sequence analysisAdd BLAST263
ChainiPRO_0000040895470 – 529Nucleocapsid protein p10Sequence analysisAdd BLAST60

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostBy similarity1

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei129 – 130Cleavage; by viral protease p14By similarity2
Sitei206 – 207Cleavage; by viral protease p14By similarity2
Sitei469 – 470Cleavage; by viral protease p14By similarity2

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PRIDEiP23090

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP23090
SMRiP23090
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili429 – 467Sequence analysisAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi176 – 179PPXY motifSequence analysis4

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Gag-p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase (Potential).Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri493 – 510CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Coiled coil, Zinc-finger

Family and domain databases

Gene3Di1.10.150.180, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR000840 G_retro_matrix
IPR036946 G_retro_matrix_sf
IPR002079 Gag_p12
IPR003036 Gag_P30
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF01140 Gag_MA, 1 hit
PF01141 Gag_p12, 1 hit
PF02093 Gag_p30, 1 hit
PF00098 zf-CCHC, 1 hit
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 1 hit
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF57756 SSF57756, 1 hit
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23090-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQTVTTPLS LTLDHWKDVQ CIASNQSVDV KRRRWVTFCS VEWPSFDVGW
60 70 80 90 100
PLDGTFNLDI ILQVKSKVTC PGPHGHPDQV PYIVTWEALV YHPPPWVKPF
110 120 130 140 150
VSPKPFPLST LPFSPPGPSA HPPSRSDLYT ALIPSKPPKS RVLPPNGGPL
160 170 180 190 200
IDLLTENLPN LPPLSKGPVK KRRPPPPRYS PPNPMESRVR GRRDPPAADS
210 220 230 240 250
TSSQAFPLRM GGDGQLQYWP FSSSDLYNWK NNNPSFSEDP GKLTALIESV
260 270 280 290 300
LTTHQPTWDD CQQLLGTLLT GEEKQRVLLE ARKAVRGNDG RPTQLPNEVN
310 320 330 340 350
SAFPLERPDW NYSTPEGRNH LVLYRQLLLA GLHNAGRSPT NLAKVKRITQ
360 370 380 390 400
GPNESPSAFL ERLKEAYRRY TPYDPEDPGQ ETNVSMSFIW QSAPDIGRKL
410 420 430 440 450
ERLEDLKSKT LGDLVREAEK IFNKRETPEE REERIRRETE EKEERRRAED
460 470 480 490 500
EQREKERDRR RHREMSKFLA TVVTGQRQDR QGGERRRPQL DEDQCAYCKE
510 520
KGHWAKDCPK KPRGPRGPRP QTSLLTLGD
Length:529
Mass (Da):60,318
Last modified:January 23, 2007 - v3
Checksum:i4B0677A58AADDD15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14576 Genomic DNA Translation: CAA32715.1
PIRiS03901 FOMVDU

Similar proteinsi

Entry informationi

Entry nameiGAG_MLVDU
AccessioniPrimary (citable) accession number: P23090
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 97 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

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