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P23048

- NSP5_ROTHD

UniProt

P23048 - NSP5_ROTHD

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Protein

Non-structural protein 5

Gene
N/A
Organism
Rotavirus A (strain Human/United States/DS-1/1976 G2-P1B[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A) (Rotavirus A (strain DS1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in genome replication. Plays a crucial role, together with NSP2, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where core-like replication intermediates are assembled and RNA replication takes place. May regulate NSP2-RNA interactions during genome replication, since NSP5 competes with RNA for the same binding site on the NSP2 octamer. Binds to either ssRNA or dsRNA with similar affinities. Displays ATPase and autokinase activities (By similarity).By similarity

Cofactori

Magnesium for ATPase activity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921MagnesiumSequence Analysis

GO - Molecular functioni

  1. ATPase activity Source: InterPro
  2. magnesium ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 5
Short name:
NSP5
Alternative name(s):
NS26
OrganismiRotavirus A (strain Human/United States/DS-1/1976 G2-P1B[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A) (Rotavirus A (strain DS1))
Taxonomic identifieri10950 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001457: Genome

Subcellular locationi

Host cytoplasm
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198Non-structural protein 5PRO_0000149636Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671Phosphoserine; by host CK1By similarity
Modified residuei154 – 1541Phosphoserine; by hostBy similarity
Modified residuei156 – 1561Phosphoserine; by hostBy similarity
Modified residuei164 – 1641Phosphoserine; by hostBy similarity
Modified residuei166 – 1661Phosphoserine; by hostBy similarity

Post-translational modificationi

O-glycosylated.By similarity
Hyperphosphorylated on serine residues, when in dimeric form. Ser-67 phosphorylation by CK1 is required for the hyperphosphorylation of NSP5 dimer. Impaired phosphorylation is associated with a profound morphological change in virus factories and a moderate decrease in virus replication (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6 (By similarity).By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4848Interaction with VP1By similarityAdd
BLAST
Regioni189 – 19810Homodimerization and interaction with NSP6By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 101100Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP5 family.Curated

Family and domain databases

InterProiIPR002512. Rotavirus_A/C_NSP5.
[Graphical view]
PfamiPF01525. Rota_NS26. 1 hit.
[Graphical view]
PIRSFiPIRSF004006. Rota_NS26. 1 hit.

Sequencei

Sequence statusi: Complete.

P23048-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLSIDVTGL PSISSSVYKN ESSSTTSTIS GKSIGRSEQY ISPDAEAFRK
60 70 80 90 100
YMLSKSPEDI GPSDSASNDP LTSFSIRSNA VKTNADAGVS MDSSAQSRPS
110 120 130 140 150
SDIGYDQMDF SLNKGIKFDA TVDSSISIST TSKKEKSKNK NKYKKCYPKI
160 170 180 190
EAESDSDDYI LDDSDSDDGK CKNCKYKKKY FALRLRMKQV AMQLIKDL
Length:198
Mass (Da):21,741
Last modified:November 1, 1991 - v1
Checksum:i0E4D75A0EE588A14
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91G → S in ABV53258. (PubMed:18786998)Curated
Sequence conflicti49 – 491R → N in ABV53258. (PubMed:18786998)Curated
Sequence conflicti138 – 1381K → KQE in ABV53258. (PubMed:18786998)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33608 Genomic RNA. Translation: AAA47327.1.
EF672583 Genomic RNA. Translation: ABV53258.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33608 Genomic RNA. Translation: AAA47327.1 .
EF672583 Genomic RNA. Translation: ABV53258.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002512. Rotavirus_A/C_NSP5.
[Graphical view ]
Pfami PF01525. Rota_NS26. 1 hit.
[Graphical view ]
PIRSFi PIRSF004006. Rota_NS26. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of gene 11 equivalents from 'short' and 'super short' strains of rotavirus."
    Matsui S.M., Mackow E.R., Matsuno S., Paul P.S., Greenberg H.B.
    J. Virol. 64:120-124(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
    Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
    J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSP5_ROTHD
AccessioniPrimary (citable) accession number: P23048
Secondary accession number(s): B3SRT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 29, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3