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P23048 (NSP5_ROTHD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 5

Short name=NSP5
Alternative name(s):
NS26
OrganismRotavirus A (strain Human/United States/DS-1/1976 G2-P1B[4]-I2-R2-C2-M2-A2-N2-T2-E2-H2) (RV-A) (Rotavirus A (strain DS1)) [Complete proteome]
Taxonomic identifier10950 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length198 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in genome replication. Plays a crucial role, together with NSP2, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where core-like replication intermediates are assembled and RNA replication takes place. May regulate NSP2-RNA interactions during genome replication, since NSP5 competes with RNA for the same binding site on the NSP2 octamer. Binds to either ssRNA or dsRNA with similar affinities. Displays ATPase and autokinase activities By similarity.

Cofactor

Magnesium for ATPase activity By similarity.

Subunit structure

Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6 By similarity.

Subcellular location

Host cytoplasm. Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

Post-translational modification

O-glycosylated By similarity.

Hyperphosphorylated on serine residues, when in dimeric form. Ser-67 phosphorylation by CK1 is required for the hyperphosphorylation of NSP5 dimer. Impaired phosphorylation is associated with a profound morphological change in virus factories and a moderate decrease in virus replication By similarity.

Sequence similarities

Belongs to the rotavirus A NSP5 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 198198Non-structural protein 5
PRO_0000149636

Regions

Region1 – 4848Interaction with VP1 By similarity
Region189 – 19810Homodimerization and interaction with NSP6 By similarity
Compositional bias2 – 101100Ser-rich

Sites

Metal binding921Magnesium Potential

Amino acid modifications

Modified residue671Phosphoserine; by host CK1 By similarity
Modified residue1541Phosphoserine; by host By similarity
Modified residue1561Phosphoserine; by host By similarity
Modified residue1641Phosphoserine; by host By similarity
Modified residue1661Phosphoserine; by host By similarity

Experimental info

Sequence conflict91G → S in ABV53258. Ref.2
Sequence conflict491R → N in ABV53258. Ref.2
Sequence conflict1381K → KQE in ABV53258. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P23048 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 0E4D75A0EE588A14

FASTA19821,741
        10         20         30         40         50         60 
MSLSIDVTGL PSISSSVYKN ESSSTTSTIS GKSIGRSEQY ISPDAEAFRK YMLSKSPEDI 

        70         80         90        100        110        120 
GPSDSASNDP LTSFSIRSNA VKTNADAGVS MDSSAQSRPS SDIGYDQMDF SLNKGIKFDA 

       130        140        150        160        170        180 
TVDSSISIST TSKKEKSKNK NKYKKCYPKI EAESDSDDYI LDDSDSDDGK CKNCKYKKKY 

       190 
FALRLRMKQV AMQLIKDL 

« Hide

References

[1]"Sequence analysis of gene 11 equivalents from 'short' and 'super short' strains of rotavirus."
Matsui S.M., Mackow E.R., Matsuno S., Paul P.S., Greenberg H.B.
J. Virol. 64:120-124(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Group A human rotavirus genomics: evidence that gene constellations are influenced by viral protein interactions."
Heiman E.M., McDonald S.M., Barro M., Taraporewala Z.F., Bar-Magen T., Patton J.T.
J. Virol. 82:11106-11116(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M33608 Genomic RNA. Translation: AAA47327.1.
EF672583 Genomic RNA. Translation: ABV53258.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002512. Rotavirus_A/C_NSP5.
[Graphical view]
PfamPF01525. Rota_NS26. 1 hit.
[Graphical view]
PIRSFPIRSF004006. Rota_NS26. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP5_ROTHD
AccessionPrimary (citable) accession number: P23048
Secondary accession number(s): B3SRT3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 3, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families