P23044 (GUN_ROBSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 1
EC=3.2.1.4

Alternative name(s):
Carboxymethyl-cellulase I
Short name=CMCase I
Endo-1,4-beta-glucanase
Endoglucanase I

Gene names

Name:

eg 1

Organism

Robillarda sp. (strain Y-20)

Taxonomic identifier

72589 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › mitosporic Ascomycota › Robillarda

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Active towards carboxymethyl cellulose.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Pathway	Glycan metabolism; cellulose degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.
Sequence caution	The sequence described in Ref.2 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Ref.2
	Chain	18 – 385	368	Endoglucanase 1		PRO_0000184049
Sites
	Active site	176	1	Proton donor By similarity
	Active site	284	1	Nucleophile By similarity
Amino acid modifications
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	140	1	N-linked (GlcNAc...) Potential
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Glycosylation	237	1	N-linked (GlcNAc...) Potential
	Glycosylation	289	1	N-linked (GlcNAc...) Potential
	Glycosylation	331	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	27 – 28	2	SS → GN AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P23044 [UniParc]. Last modified February 20, 2007. Version 2. Checksum: 149604D42369AD33	FASTA	385	41,428
	10 20 30 40 50 60 MKLVFSALAS LLSGASATIY YAGVAESSGE FGVWSATQTP GTGLPGRFGV DYAFISEAAV 70 80 90 100 110 120 DVHVDQNHLN LFRVAFLLER MCPPATGLGA AFNETHFDYF KEAVDYITVT KGAYAILDPH 130 140 150 160 170 180 NYMRYNDPSY QPFSGSVIGN TSDSTAATTE QFGEFWGELA SRFNDNERVI FGLMNEPHDM 190 200 210 220 230 240 ATSLVLANNQ AAIDAIRAAN ASNLIIMPGN SWTGGHSWTE GSDPSSALLN QFKDPLNNTA 250 260 270 280 290 300 IDIHEYLDYD FSGGHLECVS DPETNLAALT AWLKENNLKA FITEFGGSNS TSCQEMLPDL 310 320 330 340 350 360 INYMADNAEY IGWTAWAAGP FWGPNSPCCT NSTQLGSLEP GSTAVDGSPG LYDTVWLPVI 370 380 QPLVPTELQW SGPASISGGE LTSRA

References

[1]	"Endoglucanase gene from cellulolytic fungi, Robillarda sp. Y-20." Kashiwagi Y. Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and sequencing of the endo-cellulase cDNA from Robillarda sp. Y-20." Yoshigi N., Taniguchi H., Sasaki T. J. Biochem. 108:388-392(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-385, PROTEIN SEQUENCE OF 18-35.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB030819 Genomic DNA. Translation: BAA90480.1.
3D structure databases
ProteinModelPortal	P23044.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
mycoCLAP	EGL5A_ROBSP.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00696.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN_ROBSP

Accession

Primary (citable) accession number: P23044
Secondary accession number(s): Q9P981

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	February 20, 2007
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program