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P23044

- GUN_ROBSP

UniProt

P23044 - GUN_ROBSP

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Protein
Endoglucanase 1
Gene
eg 1
Organism
Robillarda sp. (strain Y-20)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Active towards carboxymethyl cellulose.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei176 – 1761Proton donor By similarity
Active sitei284 – 2841Nucleophile By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayiUPA00696.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.
mycoCLAPiEGL5A_ROBSP.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 1 (EC:3.2.1.4)
Alternative name(s):
Carboxymethyl-cellulase I
Short name:
CMCase I
Endo-1,4-beta-glucanase
Endoglucanase I
Gene namesi
Name:eg 1
OrganismiRobillarda sp. (strain Y-20)
Taxonomic identifieri72589 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotamitosporic AscomycotaRobillarda

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17171 Publication
Add
BLAST
Chaini18 – 385368Endoglucanase 1
PRO_0000184049Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi93 – 931N-linked (GlcNAc...) Reviewed prediction
Glycosylationi140 – 1401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi200 – 2001N-linked (GlcNAc...) Reviewed prediction
Glycosylationi237 – 2371N-linked (GlcNAc...) Reviewed prediction
Glycosylationi289 – 2891N-linked (GlcNAc...) Reviewed prediction
Glycosylationi331 – 3311N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliP23044.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23044-1 [UniParc]FASTAAdd to Basket

« Hide

MKLVFSALAS LLSGASATIY YAGVAESSGE FGVWSATQTP GTGLPGRFGV    50
DYAFISEAAV DVHVDQNHLN LFRVAFLLER MCPPATGLGA AFNETHFDYF 100
KEAVDYITVT KGAYAILDPH NYMRYNDPSY QPFSGSVIGN TSDSTAATTE 150
QFGEFWGELA SRFNDNERVI FGLMNEPHDM ATSLVLANNQ AAIDAIRAAN 200
ASNLIIMPGN SWTGGHSWTE GSDPSSALLN QFKDPLNNTA IDIHEYLDYD 250
FSGGHLECVS DPETNLAALT AWLKENNLKA FITEFGGSNS TSCQEMLPDL 300
INYMADNAEY IGWTAWAAGP FWGPNSPCCT NSTQLGSLEP GSTAVDGSPG 350
LYDTVWLPVI QPLVPTELQW SGPASISGGE LTSRA 385
Length:385
Mass (Da):41,428
Last modified:February 20, 2007 - v2
Checksum:i149604D42369AD33
GO

Sequence cautioni

The sequence described in 1 Publication differs from that shown. Reason: Frameshift at several positions.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 282SS → GN AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030819 Genomic DNA. Translation: BAA90480.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB030819 Genomic DNA. Translation: BAA90480.1 .

3D structure databases

ProteinModelPortali P23044.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.
mycoCLAPi EGL5A_ROBSP.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00696 .

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Endoglucanase gene from cellulolytic fungi, Robillarda sp. Y-20."
    Kashiwagi Y.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and sequencing of the endo-cellulase cDNA from Robillarda sp. Y-20."
    Yoshigi N., Taniguchi H., Sasaki T.
    J. Biochem. 108:388-392(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 33-385, PROTEIN SEQUENCE OF 18-35.

Entry informationi

Entry nameiGUN_ROBSP
AccessioniPrimary (citable) accession number: P23044
Secondary accession number(s): Q9P981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: February 20, 2007
Last modified: October 16, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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