P23034 (AAT_BACY2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Aspartate aminotransferase Short name=AspAT EC=2.6.1.1 Alternative name(s): Transaminase A
Organism	Bacillus sp. (strain YM-2)
Taxonomic identifier	72580 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Biophysicochemical properties	Temperature dependence: Optimum temperature is 70 degrees Celsius. Thermostable.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-aminocyclopropane-1-carboxylate synthase activity Inferred from electronic annotation. Source: InterPro L-aspartate:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC L-phenylalanine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Aspartate aminotransferase		PRO_0000123834
Sites
	Binding site	40	1	Aspartate; via amide nitrogen By similarity
	Binding site	126	1	Aspartate By similarity
	Binding site	176	1	Aspartate By similarity
Amino acid modifications
	Modified residue	239	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	388	1	D → L AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P23034 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 0384C74F655F85F4	FASTA	392	42,662
	10 20 30 40 50 60 MKELLANRVK TLTPSTTLAI TAKAKEMKAQ GIDVIGLGAG EPDFNTPQNI MDAAIDSMQQ 70 80 90 100 110 120 GYTKYTPSGG LPALKQAIIE KFKRDNQLEY KPNEIIVGVG AKHVLYTLFQ VILNEGDEVI 130 140 150 160 170 180 IPIPYWVSYP EQVKLAGGVP VYIEATSEQN YKITAEQLKN AITDKTKAVI INSPSNPTGM 190 200 210 220 230 240 VYTREELEDI AKIALENNIL IVSDEIYEKL LYNGAEHFSI AQISEEVKAQ TIVINGVSKS 250 260 270 280 290 300 HSMTGWRIGY AAGNADIINA MTDLASHSTS NPTTASQYAA IEAYNGPQDS VEEMRKAFES 310 320 330 340 350 360 RLETIYPKLS AIPGFKVVKP QGAFYLLPDV SEAAQKTGFA SVDEFASALL TEANVAVIPG 370 380 390 SGFGAPSTIR ISYATSLNLI EEAIERIDRF VK

References

[1]	"Thermostable aspartate aminotransferase from a thermophilic Bacillus species. Gene cloning, sequence determination, and preliminary X-ray characterization." Sung M.H., Tanizawa K., Tanaka H., Kuramitsu S., Kagamiyama H., Hirotsu K., Okamoto A., Higuchi T., Soda K. J. Biol. Chem. 266:2567-2572(1991) [PubMed: 1990006] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Purification and characterization of thermostable aspartate aminotransferase from a thermophilic Bacillus species." Sung M.H., Tanizawa K., Tanaka H., Kuramitsu S., Kagamiyama H., Soda K. J. Bacteriol. 172:1345-1351(1990) [PubMed: 2155199] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-24 AND 387-392.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59430 Genomic DNA. Translation: AAA22250.1.
3D structure databases
ProteinModelPortal	P23034.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001176. ACC_synthase. IPR004839. Aminotransferase_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00753. ACCSYNTHASE.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAT_BACY2

Accession

Primary (citable) accession number: P23034

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	November 16, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program