Reviewed, UniProtKB/Swiss-Prot P23029 (RIP2_TRIKI)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein TAP-29     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Trichosanthes kirilowii (Mongolian snake-gourd)
Taxonomic identifier	3677 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Trichosanthes

Protein attributes

Sequence length	45 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Capable of inhibiting HIV-1 infection and replication. It inactivates eukaryotic 60S ribosomal subunits.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›45	›45	Ribosome-inactivating protein TAP-29		PRO_0000221409
Experimental info
	Non-terminal residue	45	1

Sequences

	Sequence		Length	Mass (Da)
	P23029-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: EE1178150C1CF7B7	FASTA	45	5,041
	10 20 30 40 DVSFRLSGAT SKKKVYFISN LRKALPNEKK LYDIPLVRSS XSGSK

References

[1]

"TAP 29: an anti-human immunodeficiency virus protein from Trichosanthes kirilowii that is nontoxic to intact cells." Lee-Huang S., Huang P.L., Kung H.-F., Li B.-Q., Huang P.L., Huang P., Huang H.I., Chen H.-C. Proc. Natl. Acad. Sci. U.S.A. 88:6570-6574(1991) [PubMed: 1713684] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Tuberous root.

Cross-references

Sequence databases
PIR	A39598.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 273574.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. [Graphical view]
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP2_TRIKI

Accession

Primary (citable) accession number: P23029

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)