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P23024

- TCPA_VIBCL

UniProt

P23024 - TCPA_VIBCL

Protein

Toxin coregulated pilin

Gene

tcpA

Organism
Vibrio cholerae
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Constituent of pili, which may be involved in adhesion of V.cholerae to the host intestinal epithelium.

    GO - Biological processi

    1. pathogenesis Source: InterPro

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Toxin coregulated pilin
    Alternative name(s):
    Pilus colonization factor
    Gene namesi
    Name:tcpA
    OrganismiVibrio cholerae
    Taxonomic identifieri666 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular organelle Source: InterPro
    2. pilus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Fimbrium

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 2525Atypical leader peptide1 PublicationPRO_0000024192Add
    BLAST
    Chaini26 – 224199Toxin coregulated pilinPRO_0000024193Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei26 – 261N-methylmethionineSequence Analysis
    Disulfide bondi145 ↔ 211

    Keywords - PTMi

    Disulfide bond, Methylation

    Structurei

    Secondary structure

    1
    224
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi56 – 7621
    Helixi77 – 793
    Helixi88 – 10013
    Helixi106 – 1094
    Turni112 – 1143
    Beta strandi115 – 1173
    Beta strandi119 – 1257
    Beta strandi128 – 14013
    Helixi142 – 15211
    Helixi153 – 1553
    Beta strandi157 – 1659
    Helixi169 – 1724
    Turni175 – 1773
    Helixi182 – 1843
    Beta strandi185 – 1906
    Helixi204 – 2096
    Beta strandi213 – 2153
    Beta strandi217 – 2237

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OQVX-ray1.30A/B/C54-224[»]
    1QQZmodel-A/B/C/D/E/F26-222[»]
    ProteinModelPortaliP23024.
    SMRiP23024. Positions 54-224.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23024.

    Family & Domainsi

    Domaini

    The leader sequence region and some other sequence particularities suggest that TcpA may represent a novel class of pilin, and imply the existence of a novel signal peptidase.

    Family and domain databases

    InterProiIPR012902. N_methyl_site.
    IPR010271. TcpA.
    [Graphical view]
    PfamiPF05946. TcpA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
    PROSITEiPS00409. PROKAR_NTER_METHYL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23024-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQLLKQLFKK KFVKEEHDKK TGQEGMTLLE VIIVLGIMGV VSAGVVTLAQ    50
    RAIDSQIMTK AAQSLNSIQV ALTQTYRGLG NYPATADATA ASKLTSGLVS 100
    LGKISSDEAK NPFNGTNMNI FSFPRNAAAN KAFAISVDGL TQAQCKTLIT 150
    SVGDMFPYIA IKAGGAVALA DLGDFENSAA AAETGVGVIK SIAPASKNLD 200
    LTNITHVEKL CKGTAPFGVA FGNS 224
    Length:224
    Mass (Da):23,328
    Last modified:November 1, 1991 - v1
    Checksum:iE7468D3E272276B5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti57 – 571I → N in CAA45455. (PubMed:8621096)Curated
    Sequence conflicti114 – 1141N → I in CAA45455. (PubMed:8621096)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33514 Genomic DNA. Translation: AAA88688.1.
    X64098 Genomic DNA. Translation: CAA45455.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33514 Genomic DNA. Translation: AAA88688.1 .
    X64098 Genomic DNA. Translation: CAA45455.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OQV X-ray 1.30 A/B/C 54-224 [» ]
    1QQZ model - A/B/C/D/E/F 26-222 [» ]
    ProteinModelPortali P23024.
    SMRi P23024. Positions 54-224.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P23024.

    Family and domain databases

    InterProi IPR012902. N_methyl_site.
    IPR010271. TcpA.
    [Graphical view ]
    Pfami PF05946. TcpA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02532. IV_pilin_GFxxxE. 1 hit.
    PROSITEi PS00409. PROKAR_NTER_METHYL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the structural gene, tcpA, for a major pilin subunit of Vibrio cholerae."
      Faast R., Ogierman M.A., Stroeher U.H., Manning P.A.
      Gene 85:227-231(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Classical Inaba Z17561 / Serotype O1.
    2. "Comparison of the promoter proximal regions of the toxin-co-regulated tcp gene cluster in classical and El Tor strains of Vibrio cholerae O1."
      Ogierman M.A., Voss E., Meaney C., Faast R., Attridge S.R., Manning P.A.
      Gene 170:9-16(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Classical Inaba Z17561 / Serotype O1.
    3. "Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin."
      Taylor R.K., Miller V.L., Furlong D.B., Mekalanos J.J.
      Proc. Natl. Acad. Sci. U.S.A. 84:2833-2837(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 26-50.
    4. "Type IV pilin structure and assembly: X-ray and EM analyses of Vibrio cholerae toxin-coregulated pilus and Pseudomonas aeruginosa PAK pilin."
      Craig L., Taylor R.K., Pique M.E., Adair B.D., Arvai A.S., Singh M., Lloyd S.J., Shin D.S., Getzoff E.D., Yeager M., Forest K.T., Tainer J.A.
      Mol. Cell 11:1139-1150(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 33-224.

    Entry informationi

    Entry nameiTCPA_VIBCL
    AccessioniPrimary (citable) accession number: P23024
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3