P23024 (TCPA_VIBCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Toxin coregulated pilin

Alternative name(s):
Pilus colonization factor

Gene names

Name:

tcpA

Organism

Vibrio cholerae

Taxonomic identifier

666 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Constituent of pili, which may be involved in adhesion of V.cholerae to the host intestinal epithelium.
Subcellular location	Fimbrium.
Domain	The leader sequence region and some other sequence particularities suggest that TcpA may represent a novel class of pilin, and imply the existence of a novel signal peptidase.

Ontologies

Keywords
Cellular component	Fimbrium
PTM	Disulfide bond Methylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular organelle Inferred from electronic annotation. Source: InterPro pilus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Propeptide

1 – 25

25

Atypical leader peptide

PRO_0000024192

Chain

26 – 224

199

Toxin coregulated pilin

PRO_0000024193

Amino acid modifications

Modified residue

26

1

N-methylmethionine Potential

Disulfide bond

145 ↔ 211

Experimental info

Sequence conflict

57

1

I → N in CAA45455. Ref.2

Sequence conflict

114

1

N → I in CAA45455. Ref.2

Secondary structure

1

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224

Helix Strand Turn

Details...

Helix

56 – 76

21

Helix

77 – 79

3

Helix

88 – 100

13

Helix

106 – 109

4

Turn

112 – 114

3

Beta strand

115 – 117

3

Beta strand

119 – 125

7

Beta strand

128 – 140

13

Helix

142 – 152

11

Helix

153 – 155

3

Beta strand

157 – 165

9

Helix

169 – 172

4

Turn

175 – 177

3

Helix

182 – 184

3

Beta strand

185 – 190

6

Helix

204 – 209

6

Beta strand

213 – 215

3

Beta strand

217 – 223

7

Sequences

	Sequence		Length	Mass (Da)
	P23024 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: E7468D3E272276B5	FASTA	224	23,328
	10 20 30 40 50 60 MQLLKQLFKK KFVKEEHDKK TGQEGMTLLE VIIVLGIMGV VSAGVVTLAQ RAIDSQIMTK 70 80 90 100 110 120 AAQSLNSIQV ALTQTYRGLG NYPATADATA ASKLTSGLVS LGKISSDEAK NPFNGTNMNI 130 140 150 160 170 180 FSFPRNAAAN KAFAISVDGL TQAQCKTLIT SVGDMFPYIA IKAGGAVALA DLGDFENSAA 190 200 210 220 AAETGVGVIK SIAPASKNLD LTNITHVEKL CKGTAPFGVA FGNS

References

[1]	"Nucleotide sequence of the structural gene, tcpA, for a major pilin subunit of Vibrio cholerae." Faast R., Ogierman M.A., Stroeher U.H., Manning P.A. Gene 85:227-231(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Classical Inaba Z17561 / Serotype O1.
[2]	"Comparison of the promoter proximal regions of the toxin-co-regulated tcp gene cluster in classical and El Tor strains of Vibrio cholerae O1." Ogierman M.A., Voss E., Meaney C., Faast R., Attridge S.R., Manning P.A. Gene 170:9-16(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Classical Inaba Z17561 / Serotype O1.
[3]	"Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin." Taylor R.K., Miller V.L., Furlong D.B., Mekalanos J.J. Proc. Natl. Acad. Sci. U.S.A. 84:2833-2837(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-50.
[4]	"Type IV pilin structure and assembly: X-ray and EM analyses of Vibrio cholerae toxin-coregulated pilus and Pseudomonas aeruginosa PAK pilin." Craig L., Taylor R.K., Pique M.E., Adair B.D., Arvai A.S., Singh M., Lloyd S.J., Shin D.S., Getzoff E.D., Yeager M., Forest K.T., Tainer J.A. Mol. Cell 11:1139-1150(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 33-224.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M33514 Genomic DNA. Translation: AAA88688.1.
X64098 Genomic DNA. Translation: CAA45455.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OQV	X-ray	1.30	A/B/C	54-224	[»]
1QQZ	model	-	A/B/C/D/E/F	26-222	[»]

ProteinModelPortal

P23024.

SMR

P23024. Positions 54-224.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR012902. N_methyl_site.
IPR010271. TcpA.
[Graphical view]

Pfam

PF05946. TcpA. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02532. IV_pilin_GFxxxE. 1 hit.

PROSITE

PS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P23024.

Entry information

Entry name

TCPA_VIBCL

Accession

Primary (citable) accession number: P23024

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program