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Protein

Toxin coregulated pilin

Gene

tcpA

Organism
Vibrio cholerae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of pili, which may be involved in adhesion of V.cholerae to the host intestinal epithelium.

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin coregulated pilin
Alternative name(s):
Pilus colonization factor
Gene namesi
Name:tcpA
OrganismiVibrio cholerae
Taxonomic identifieri666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Fimbrium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 2525Atypical leader peptide1 PublicationPRO_0000024192Add
BLAST
Chaini26 – 224199Toxin coregulated pilinPRO_0000024193Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei26 – 261N-methylmethioninePROSITE-ProRule annotation
Disulfide bondi145 ↔ 211

Keywords - PTMi

Disulfide bond, Methylation

Interactioni

Protein-protein interaction databases

STRINGi345073.VC0395_A0353.

Structurei

Secondary structure

1
224
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi56 – 7621Combined sources
Helixi77 – 793Combined sources
Helixi88 – 10013Combined sources
Helixi106 – 1094Combined sources
Turni112 – 1143Combined sources
Beta strandi115 – 1173Combined sources
Beta strandi119 – 1257Combined sources
Beta strandi128 – 14013Combined sources
Helixi142 – 15211Combined sources
Helixi153 – 1553Combined sources
Beta strandi157 – 1659Combined sources
Helixi169 – 1724Combined sources
Turni175 – 1773Combined sources
Helixi182 – 1843Combined sources
Beta strandi185 – 1906Combined sources
Helixi204 – 2096Combined sources
Beta strandi213 – 2153Combined sources
Beta strandi217 – 2237Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OQVX-ray1.30A/B/C54-224[»]
1QQZmodel-A/B/C/D/E/F26-222[»]
ProteinModelPortaliP23024.
SMRiP23024. Positions 54-224.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23024.

Family & Domainsi

Domaini

The leader sequence region and some other sequence particularities suggest that TcpA may represent a novel class of pilin, and imply the existence of a novel signal peptidase.

Family and domain databases

InterProiIPR012902. N_methyl_site.
IPR010271. TcpA.
[Graphical view]
PfamiPF05946. TcpA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEiPS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23024-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLLKQLFKK KFVKEEHDKK TGQEGMTLLE VIIVLGIMGV VSAGVVTLAQ
60 70 80 90 100
RAIDSQIMTK AAQSLNSIQV ALTQTYRGLG NYPATADATA ASKLTSGLVS
110 120 130 140 150
LGKISSDEAK NPFNGTNMNI FSFPRNAAAN KAFAISVDGL TQAQCKTLIT
160 170 180 190 200
SVGDMFPYIA IKAGGAVALA DLGDFENSAA AAETGVGVIK SIAPASKNLD
210 220
LTNITHVEKL CKGTAPFGVA FGNS
Length:224
Mass (Da):23,328
Last modified:November 1, 1991 - v1
Checksum:iE7468D3E272276B5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571I → N in CAA45455 (PubMed:8621096).Curated
Sequence conflicti114 – 1141N → I in CAA45455 (PubMed:8621096).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33514 Genomic DNA. Translation: AAA88688.1.
X64098 Genomic DNA. Translation: CAA45455.1.
RefSeqiWP_001176378.1. NZ_JFGR01000021.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33514 Genomic DNA. Translation: AAA88688.1.
X64098 Genomic DNA. Translation: CAA45455.1.
RefSeqiWP_001176378.1. NZ_JFGR01000021.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OQVX-ray1.30A/B/C54-224[»]
1QQZmodel-A/B/C/D/E/F26-222[»]
ProteinModelPortaliP23024.
SMRiP23024. Positions 54-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi345073.VC0395_A0353.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP23024.

Family and domain databases

InterProiIPR012902. N_methyl_site.
IPR010271. TcpA.
[Graphical view]
PfamiPF05946. TcpA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEiPS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the structural gene, tcpA, for a major pilin subunit of Vibrio cholerae."
    Faast R., Ogierman M.A., Stroeher U.H., Manning P.A.
    Gene 85:227-231(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Classical Inaba Z17561 / Serotype O1.
  2. "Comparison of the promoter proximal regions of the toxin-co-regulated tcp gene cluster in classical and El Tor strains of Vibrio cholerae O1."
    Ogierman M.A., Voss E., Meaney C., Faast R., Attridge S.R., Manning P.A.
    Gene 170:9-16(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Classical Inaba Z17561 / Serotype O1.
  3. "Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin."
    Taylor R.K., Miller V.L., Furlong D.B., Mekalanos J.J.
    Proc. Natl. Acad. Sci. U.S.A. 84:2833-2837(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-50.
  4. "Type IV pilin structure and assembly: X-ray and EM analyses of Vibrio cholerae toxin-coregulated pilus and Pseudomonas aeruginosa PAK pilin."
    Craig L., Taylor R.K., Pique M.E., Adair B.D., Arvai A.S., Singh M., Lloyd S.J., Shin D.S., Getzoff E.D., Yeager M., Forest K.T., Tainer J.A.
    Mol. Cell 11:1139-1150(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 33-224.

Entry informationi

Entry nameiTCPA_VIBCL
AccessioniPrimary (citable) accession number: P23024
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 24, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.