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Protein

Toxin coregulated pilin

Gene

tcpA

Organism
Vibrio cholerae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Constituent of pili, which may be involved in adhesion of V.cholerae to the host intestinal epithelium.

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin coregulated pilin
Alternative name(s):
Pilus colonization factor
Gene namesi
Name:tcpA
OrganismiVibrio cholerae
Taxonomic identifieri666 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Fimbrium

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000241921 – 25Atypical leader peptide1 PublicationAdd BLAST25
ChainiPRO_000002419326 – 224Toxin coregulated pilinAdd BLAST199

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26N-methylmethioninePROSITE-ProRule annotation1
Disulfide bondi145 ↔ 211

Keywords - PTMi

Disulfide bond, Methylation

Interactioni

Protein-protein interaction databases

STRINGi345073.VC0395_A0353.

Structurei

Secondary structure

1224
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi56 – 76Combined sources21
Helixi77 – 79Combined sources3
Helixi88 – 100Combined sources13
Helixi106 – 109Combined sources4
Turni112 – 114Combined sources3
Beta strandi115 – 117Combined sources3
Beta strandi119 – 125Combined sources7
Beta strandi128 – 140Combined sources13
Helixi142 – 152Combined sources11
Helixi153 – 155Combined sources3
Beta strandi157 – 165Combined sources9
Helixi169 – 172Combined sources4
Turni175 – 177Combined sources3
Helixi182 – 184Combined sources3
Beta strandi185 – 190Combined sources6
Helixi204 – 209Combined sources6
Beta strandi213 – 215Combined sources3
Beta strandi217 – 223Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OQVX-ray1.30A/B/C54-224[»]
1QQZmodel-A/B/C/D/E/F26-222[»]
ProteinModelPortaliP23024.
SMRiP23024.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23024.

Family & Domainsi

Domaini

The leader sequence region and some other sequence particularities suggest that TcpA may represent a novel class of pilin, and imply the existence of a novel signal peptidase.

Phylogenomic databases

eggNOGiENOG4105XB6. Bacteria.
COG2165. LUCA.

Family and domain databases

InterProiIPR012902. N_methyl_site.
IPR010271. TcpA.
[Graphical view]
PfamiPF07963. N_methyl. 1 hit.
PF05946. TcpA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEiPS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23024-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLLKQLFKK KFVKEEHDKK TGQEGMTLLE VIIVLGIMGV VSAGVVTLAQ
60 70 80 90 100
RAIDSQIMTK AAQSLNSIQV ALTQTYRGLG NYPATADATA ASKLTSGLVS
110 120 130 140 150
LGKISSDEAK NPFNGTNMNI FSFPRNAAAN KAFAISVDGL TQAQCKTLIT
160 170 180 190 200
SVGDMFPYIA IKAGGAVALA DLGDFENSAA AAETGVGVIK SIAPASKNLD
210 220
LTNITHVEKL CKGTAPFGVA FGNS
Length:224
Mass (Da):23,328
Last modified:November 1, 1991 - v1
Checksum:iE7468D3E272276B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57I → N in CAA45455 (PubMed:8621096).Curated1
Sequence conflicti114N → I in CAA45455 (PubMed:8621096).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33514 Genomic DNA. Translation: AAA88688.1.
X64098 Genomic DNA. Translation: CAA45455.1.
RefSeqiWP_001176378.1. NZ_LOSM01000005.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33514 Genomic DNA. Translation: AAA88688.1.
X64098 Genomic DNA. Translation: CAA45455.1.
RefSeqiWP_001176378.1. NZ_LOSM01000005.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OQVX-ray1.30A/B/C54-224[»]
1QQZmodel-A/B/C/D/E/F26-222[»]
ProteinModelPortaliP23024.
SMRiP23024.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi345073.VC0395_A0353.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105XB6. Bacteria.
COG2165. LUCA.

Miscellaneous databases

EvolutionaryTraceiP23024.

Family and domain databases

InterProiIPR012902. N_methyl_site.
IPR010271. TcpA.
[Graphical view]
PfamiPF07963. N_methyl. 1 hit.
PF05946. TcpA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02532. IV_pilin_GFxxxE. 1 hit.
PROSITEiPS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTCPA_VIBCL
AccessioniPrimary (citable) accession number: P23024
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.