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P23009

- POL2_BPMV

UniProt

P23009 - POL2_BPMV

Protein

RNA2 polyprotein

Gene
N/A
Organism
Bean-pod mottle virus (strain Kentucky G7) (BPMV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Movement protein: transports viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei446 – 4472CleavageBy similarity
    Sitei820 – 8212CleavageBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. GTP binding Source: UniProtKB-KW
    3. RNA binding Source: UniProtKB-KW
    4. structural molecule activity Source: InterPro

    GO - Biological processi

    1. transport of virus in host, cell to cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Viral movement protein

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    DNA-binding, GTP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA2 polyprotein
    Alternative name(s):
    Genome polyprotein M
    P2
    Cleaved into the following 3 chains:
    Movement protein
    Short name:
    MP
    Large coat protein
    Short name:
    LCP
    Alternative name(s):
    Coat protein VP37
    Small coat protein
    Short name:
    SCP
    Alternative name(s):
    Coat protein VP23
    OrganismiBean-pod mottle virus (strain Kentucky G7) (BPMV)
    Taxonomic identifieri31715 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesSecoviridaeComovirinaeComovirus
    Virus hostiDesmodium [TaxID: 53866]
    Glycine max (Soybean) (Glycine hispida) [TaxID: 3847]
    Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
    ProteomesiUP000007610: Genome

    Subcellular locationi

    Chain Movement protein : Host cell junctionhost plasmodesma By similarity
    Note: Assembles in tubules that are embedded within modified plasmodesmata.By similarity

    GO - Cellular componenti

    1. host cell plasmodesma Source: UniProtKB-SubCell
    2. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cell junction, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 446446Movement proteinBy similarityPRO_0000037031Add
    BLAST
    Chaini447 – 820374Large coat proteinBy similarityPRO_0000037032Add
    BLAST
    Chaini821 – 1018198Small coat proteinBy similarityPRO_0000037033Add
    BLAST

    Post-translational modificationi

    Specific enzymatic cleavages by RNA1 encoded picornain 3C-like protease in vivo yield mature proteins.By similarity

    Structurei

    Secondary structure

    1
    1018
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi451 – 4533
    Turni466 – 4694
    Beta strandi470 – 4789
    Beta strandi487 – 4926
    Helixi493 – 4964
    Beta strandi498 – 5003
    Helixi505 – 5106
    Beta strandi516 – 5238
    Beta strandi533 – 54210
    Helixi550 – 5534
    Beta strandi554 – 5618
    Turni563 – 5653
    Beta strandi567 – 5737
    Helixi584 – 5907
    Beta strandi593 – 6008
    Beta strandi610 – 62213
    Beta strandi628 – 6325
    Beta strandi635 – 64814
    Beta strandi650 – 6523
    Beta strandi657 – 6615
    Beta strandi667 – 6693
    Beta strandi672 – 6743
    Helixi677 – 6826
    Beta strandi685 – 69814
    Beta strandi708 – 7136
    Helixi724 – 7263
    Beta strandi727 – 7326
    Beta strandi740 – 7456
    Helixi747 – 7493
    Beta strandi754 – 7563
    Turni764 – 7663
    Beta strandi771 – 7788
    Beta strandi783 – 7853
    Beta strandi788 – 80518
    Beta strandi829 – 8357
    Turni842 – 8443
    Beta strandi846 – 8538
    Turni854 – 8574
    Beta strandi858 – 8614
    Beta strandi863 – 8653
    Beta strandi868 – 8714
    Helixi875 – 8828
    Beta strandi883 – 89715
    Beta strandi900 – 9023
    Helixi904 – 9063
    Beta strandi910 – 9178
    Beta strandi921 – 9233
    Beta strandi926 – 9316
    Beta strandi934 – 94512
    Turni948 – 9503
    Turni958 – 9614
    Beta strandi966 – 9738
    Turni974 – 9763
    Beta strandi979 – 9868
    Beta strandi991 – 9977

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BMVX-ray3.001821-1005[»]
    2447-820[»]
    1PGLX-ray2.801821-1005[»]
    2447-816[»]
    1PGWX-ray2.901821-1005[»]
    2466-816[»]
    ProteinModelPortaliP23009.
    SMRiP23009. Positions 447-1005.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23009.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.60.120.20. 2 hits.
    InterProiIPR003181. Como_LCP.
    IPR003182. RNA2_polyprotein.
    IPR029053. Viral_coat.
    IPR028919. Viral_movement.
    [Graphical view]
    PfamiPF02247. Como_LCP. 1 hit.
    PF02248. Como_SCP. 1 hit.
    PF01107. MP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23009-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFASFIFSGD NKLTEKTIFN CGDLDILVVY YTIATQFRKF LPHYIRWHLY     50
    TLLIYILPSF LTTEIKYKRN LSNIHISGLF YDNRFKFWTK HDKNLALTEE 100
    EKMEVIRNRG IPADVLAKRA HEFEKHVAHE SLKDQIPAVD KLYSTKVNKF 150
    AKIMNLRQSV VGDLKLLTDG KLYEGKHIPV SNISAGENHV VQIPLMAQEE 200
    ILSSSASDFK TAMVSKSSKP QATAMHVGAI EIIIDSFASP DCNIVGAMLL 250
    VDTYHTNPEN AVRSIFVAPF RGGRPIRVVT FPNTIVQIEP DMNSRFQLLS 300
    TTTNGDFVQG KDLAMVKVNV ACAAVGLTSS YTPTPLLESG LQKDRGLIVE 350
    YFGRMSYVAH NINQPQEKDL LEGNFSFDIK SRSRLEKVSS TKAQFVSGRT 400
    FKYDIIGAGS QSSEELSEEK IQGKAKQVDA RLRQRIDPQY NEVQAQMETN 450
    LFKLSLDDVE TPKGSMLDLK ISQSKIALPK NTVGGTILRS DLLANFLTEG 500
    NFRASVDLQR THRIKGMIKM VATVGIPENT GIALACAMNS SIRGRASSDI 550
    YTICSQDCEL WNPACTKAMT MSFNPNPCSD AWSLEFLKRT GFHCDIICVT 600
    GWTATPMQDV QVTIDWFISS QECVPRTYCV LNPQNPFVLN RWMGKLTFPQ 650
    GTSRSVKRMP LSIGGGAGAK SAILMNMPNA VLSMWRYFVG DLVFEVSKMT 700
    SPYIKCTVSF FIAFGNLADD TINFEAFPHK LVQFGEIQEK VVLKFSQEEF 750
    LTAWSTQVRP ATTLLADGCP YLYAMVHDSS VSTIPGDFVI GVKLTIIENM 800
    CAYGLNPGIS GSRLLGTIPQ SISQQTVWNQ MATVRTPLNF DSSKQSFCQF 850
    SVDLLGGGIS VDKTGDWITL VQNSPISNLL RVAAWKKGCL MVKVVMSGNA 900
    AVKRSDWASL VQVFLTNSNS TEHFDACRWT KSEPHSWELI FPIEVCGPNN 950
    GFEMWSSEWA NQTSWHLSFL VDNPKQSTTF DVLLGISQNF EIAGNTLMPA 1000
    FSVPQANARS SENAESSA 1018
    Length:1,018
    Mass (Da):113,352
    Last modified:December 1, 1992 - v2
    Checksum:iB11B35D0A7F0F872
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62738 Genomic RNA. Translation: AAA42801.1.
    PIRiA40321. GNWXG7.
    RefSeqiNP_612348.1. NC_003495.1.

    Genome annotation databases

    GeneIDi956616.

    Cross-referencesi

    Web resourcesi

    Virus Particle ExploreR db

    Icosahedral capsid structure

    Virus Particle ExploreR db

    Icosahedral capsid structure

    Virus Particle ExploreR db

    Icosahedral capsid structure

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62738 Genomic RNA. Translation: AAA42801.1 .
    PIRi A40321. GNWXG7.
    RefSeqi NP_612348.1. NC_003495.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BMV X-ray 3.00 1 821-1005 [» ]
    2 447-820 [» ]
    1PGL X-ray 2.80 1 821-1005 [» ]
    2 447-816 [» ]
    1PGW X-ray 2.90 1 821-1005 [» ]
    2 466-816 [» ]
    ProteinModelPortali P23009.
    SMRi P23009. Positions 447-1005.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956616.

    Miscellaneous databases

    EvolutionaryTracei P23009.

    Family and domain databases

    Gene3Di 2.60.120.20. 2 hits.
    InterProi IPR003181. Como_LCP.
    IPR003182. RNA2_polyprotein.
    IPR029053. Viral_coat.
    IPR028919. Viral_movement.
    [Graphical view ]
    Pfami PF02247. Como_LCP. 1 hit.
    PF02248. Como_SCP. 1 hit.
    PF01107. MP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the nucleotide sequence of bean pod mottle virus middle component RNA."
      Macfarlane S.A., Shanks M., Davies J.W., Zlotnick A., Lomonossoff G.P.
      Virology 183:405-409(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Protein-RNA interactions in an icosahedral virus at 3.0-A resolution."
      Chen Z., Stauffacher C., Li Y., Schmidt T., Bomu W., Kamer G., Shanks M., Lomonossoff G., Johnson J.E.
      Science 245:154-159(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 447-1018.
    3. "Evidence for assembly-dependent folding of protein and RNA in an icosahedral virus."
      Lin T., Cavarelli J., Johnson J.E.
      Virology 314:26-33(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 447-1005.

    Entry informationi

    Entry nameiPOL2_BPMV
    AccessioniPrimary (citable) accession number: P23009
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The virus capsid is composed of 60 icosahedral units, each of which is composed of one copy each of the two coat proteins.

    Caution

    It is uncertain whether Met-1 or Met-103 is the initiator.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3