Reviewed, UniProtKB/Swiss-Prot P23006 (DHMH_PARVE)

Names and origin

Protein names

Recommended name:
    Methylamine dehydrogenase heavy chain
      Short name=MADH
    EC=1.4.99.3

Gene names

Name:	mauB
Synonyms:	madA

Organism

Paracoccus versutus (Thiobacillus versutus)

Taxonomic identifier

34007 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Methylamine dehydrogenase carries out the oxidation of methylamine. Electrons are passed from methylamine dehydrogenase to amicyanin.
Catalytic activity	RCH2NH2 + H2O + acceptor = RCHO + NH3 + reduced acceptor.
Subunit structure	Tetramer of two light and two heavy chains.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the aromatic amine dehydrogenase heavy chain family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW methylamine metabolic process Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 426	395	Methylamine dehydrogenase heavy chain		PRO_0000025581
Amino acid modifications
	Disulfide bond	221 ↔ 236

Sequences

	Sequence		Length	Mass (Da)
	P23006-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 4ECDF8F6A7AE8696	FASTA	426	46,388
	10 20 30 40 50 60 MASARESTPR YLTLIGATLA CSALALGAAQ AQTEPAEPEA PAETAAADAA GQTEGQRGAA 70 80 90 100 110 120 EAAAALAAGE ADEPVILEAP APDARRVYIQ DPAHFAAITQ QFVIDGSTGR ILGMTDGGFL 130 140 150 160 170 180 PHPVAAEDGS FFAQASTVFE RIARGKRTDY VEVFDPVTFL PIADIELPDA PRFLVGTYQW 190 200 210 220 230 240 MNALTPDNKN LLFYQFSPAP AVGVVDLEGK TFDRMLDVPD CYHIFPASPT VFYMNCRDGS 250 260 270 280 290 300 LARVDFADGE TKVTNTEVFH TEDELLINHP AFSLRSGRLV WPTYTGKIFQ ADLTAEGATF 310 320 330 340 350 360 RAPIEALTEA ERADDWRPGG WQQTAYHRQS DRIYLLVDQR DEWKHKAASR FVVVLNAETG 370 380 390 400 410 420 ERINKIELGH EIDSINVSQD AEPLLYALSA GTQTLHIYDA ATGEELRSVD QLGRGPQIIT THDMDS

References

[1]	"Cloning and sequencing of the gene coding for the large subunit of methylamine dehydrogenase from Thiobacillus versutus." Huitema F., van Beeumen J., van Driessche G., Duine J.A., Canters G.W. J. Bacteriol. 175:6254-6259(1993) [PubMed: 8407797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure of quinoprotein methylamine dehydrogenase at 2.25-A resolution." Vellieux F.M.D., Huitema F., Groendijk H., Kalk K.H., Jzn J.F., Jongejan J.A., Duine J.A., Petratos K., Drenth J., Hol W.G.J. EMBO J. 8:2171-2178(1989) [PubMed: 2792083] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).
[3]	"Structure determination of quinoprotein methylamine dehydrogenase from Thiobacillus versutus." Vellieux F.M.D., Kalk K.H., Hol W.G.J. Acta Crystallogr. B 46:806-823(1990) [PubMed: 2085423] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L08575 Genomic DNA. Translation: AAA72335.1.

PIR

A36934.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MAE	X-ray	2.80	H	59-400	[»]
1MAF	X-ray	2.60	H	59-400	[»]
2MAD	X-ray	2.25	H	59-400	[»]
3C75	X-ray	2.50	H/J	1-426	[»]

SMR

P23006. Positions 72-424.

ModBase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MON-3901.

BRENDA

1.4.99.3. 277244.

Family and domain databases

InterPro

IPR013476. MeN_DH_hvc.
IPR009451. Metamine_DH_Hsu.
IPR015943. WD40/YVTN_repeat-like.
[Graphical view]

Gene3D

G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.

Pfam

PF06433. Me-amine-dh_H. 1 hit.
[Graphical view]

PIRSF

PIRSF017797. TTQ_MADH_Hv. 1 hit.

TIGRFAMs

TIGR02658. TTQ_MADH_Hv. 1 hit.

ProtoNet

Search...

Entry information

Entry name

DHMH_PARVE

Accession

Primary (citable) accession number: P23006
Secondary accession number(s): Q60052

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)