P23005 (CRGF_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-crystallin F

Alternative name(s):
Gamma-F-crystallin
Gamma-crystallin IVA

Gene names

Name:

CRYGF

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Crystallins are the dominant structural components of the vertebrate eye lens.
Domain	Has a two-domain beta-structure, folded into four very similar Greek key motifs.
Sequence similarities	Belongs to the beta/gamma-crystallin family. Contains 4 beta/gamma crystallin 'Greek key' domains.

Ontologies

Keywords
Domain	Repeat
Molecular function	Eye lens protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	structural constituent of eye lens Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 174

173

Gamma-crystallin F

PRO_0000057584

Regions

Domain

2 – 40

39

Beta/gamma crystallin 'Greek key' 1

Domain

41 – 83

43

Beta/gamma crystallin 'Greek key' 2

Domain

88 – 128

41

Beta/gamma crystallin 'Greek key' 3

Domain

129 – 171

43

Beta/gamma crystallin 'Greek key' 4

Region

84 – 87

4

Connecting peptide

Secondary structure

1

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174

Helix Strand Turn

Details...

Beta strand

3 – 9

7

Turn

10 – 12

3

Beta strand

13 – 19

7

Turn

27 – 29

3

Beta strand

34 – 48

15

Helix

49 – 51

3

Beta strand

55 – 58

4

Beta strand

60 – 65

6

Turn

66 – 70

5

Beta strand

72 – 74

3

Beta strand

78 – 82

5

Beta strand

89 – 95

7

Helix

96 – 98

3

Beta strand

102 – 105

4

Turn

113 – 115

3

Beta strand

123 – 129

7

Beta strand

131 – 139

9

Beta strand

143 – 146

4

Beta strand

148 – 151

4

Helix

154 – 157

4

Beta strand

166 – 169

4

Sequences

	Sequence		Length	Mass (Da)
	P23005 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 1ED266259EB6DC70	FASTA	174	21,086
	10 20 30 40 50 60 MGKITFYEDR GFQGRHYECS SDHSNLQPYF SRCNSIRVDS GCWMLYEQPN FQGPQYFLRR 70 80 90 100 110 120 GDYPDYQQWM GLNDSIRSCR LIPHTGSHRL RIYEREDYRG QMVEITEDCS SLHDRFHFSE 130 140 150 160 170 IHSFNVLEGW WVLYEMTNYR GRQYLLRPGD YRRYHDWGAT NARVGSLRRA VDFY

References

[1]	"Packing interactions in the eye-lens. Structural analysis, internal symmetry and lattice interactions of bovine gamma IVa-crystallin." White H.E., Driessen H.P.C., Slingsby C., Moss D.S., Lindley P.F. J. Mol. Biol. 207:217-235(1989) [PubMed: 2738925] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[2]	"Towards a molecular understanding of phase separation in the lens: a comparison of the X-ray structures of two high Tc gamma-crystallins, gammaE and gammaF, with two low Tc gamma-crystallins, gammaB and gammaD." Norledge B.V., Hay R.E., Bateman O.A., Slingsby C., Driessen H.P.C. Exp. Eye Res. 65:609-630(1997) [PubMed: 9367641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

IPI

IPI00703834.

RefSeq

NP_001193748.1. NM_001206819.1.

UniGene

Bt.63318.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A45	X-ray	2.30	A	2-174	[»]
1M8U	X-ray	1.65	A	2-173	[»]

ProteinModelPortal

P23005.

SMR

P23005. Positions 2-174.

ModBase

Search...

Proteomic databases

PRIDE

P23005.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSBTAT00000020038; ENSBTAP00000020038; ENSBTAG00000046940.

GeneID

616092.

KEGG

bta:616092.

Phylogenomic databases

eggNOG

maNOG17799.

GeneTree

ENSGT00560000076658.

HOVERGEN

HBG003364.

InParanoid

P23005.

OrthoDB

EOG4D7Z6S.

PhylomeDB

P23005.

Family and domain databases

InterPro

IPR001064. Beta/gamma_crystallin.
IPR011024. G_crystallin-rel.
[Graphical view]

Gene3D

G3DSA:2.60.20.10. Crystallin. 2 hits.

Pfam

PF00030. Crystall. 2 hits.
[Graphical view]

PRINTS

PR01367. BGCRYSTALLIN.

SMART

SM00247. XTALbg. 2 hits.
[Graphical view]

SUPFAM

SSF49695. G_crystallin_SF. 1 hit.

PROSITE

PS50915. CRYSTALLIN_BETA_GAMMA. 4 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CRGF_BOVIN

Accession

Primary (citable) accession number: P23005

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 85 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program