P22990 (RIBF_PSEFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin biosynthesis protein RibF

Including the following 2 domains:

1. Riboflavin kinase
   EC=2.7.1.26
   Alternative name(s):
   Flavokinase
2. FMN adenylyltransferase
   EC=2.7.7.2
   Alternative name(s):
   FAD pyrophosphorylase
   FAD synthase

Gene names

Name:

ribF

Organism

Pseudomonas fluorescens

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas fluorescens group

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + riboflavin = ADP + FMN. ATP + FMN = diphosphate + FAD.
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
Sequence similarities	Belongs to the RibF family.

Ontologies

Keywords
Ligand	ATP-binding FAD FMN Nucleotide-binding
Molecular function	Kinase Nucleotidyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	FAD biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway FMN biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW FMN adenylyltransferase activity Inferred from electronic annotation. Source: EC riboflavin kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Riboflavin biosynthesis protein RibF		PRO_0000194141

Sequences

	Sequence		Length	Mass (Da)
	P22990 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: E4ED0EF97C064D60	FASTA	311	33,898
	10 20 30 40 50 60 MQLVRGLHNL RPEHRGCVAT IGNFDGVHRG HQAILARLRE RAVELGVPSC VVIFEPQPRE 70 80 90 100 110 120 FFTPETAPAR LALARQAATA GGEGVDRVLC LAFNQRLRSL SAAEFVDRIL VDGLGVQHLE 130 140 150 160 170 180 VGDDFHFGCD RVGDFDFLQH AGVNQGFTVE AAQTVELDGL RVSSTQVRNA LAAADFDLAE 190 200 210 220 230 240 RLLGRPFRIA GRVLHGQKLA RQLGTPTANV QLKRRRVPLT GVYLVSVDID GQSWPGVANI 250 260 270 280 290 300 GVRPTVAGDG KAHLEVHLLD FAGDLYDRRL TVVFHQKLRE EQRFASLEAL KTAINADVAA 310 ARALAAPSAH R

References

[1]	"Nucleotide sequence of the Pseudomonas fluorescens signal peptidase II gene (lsp) and flanking genes." Isaki L., Beers R., Wu H.C. J. Bacteriol. 172:6512-6517(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49323 / NCIMB 10586.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35367 Genomic DNA. Translation: AAA26021.1.
PIR	E37152.
3D structure databases
ProteinModelPortal	P22990.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00276; UER00406. UPA00277; UER00407.
Family and domain databases
Gene3D	2.40.30.30. 1 hit. 3.40.50.620. 1 hit.
InterPro	IPR015864. FAD_synthase. IPR023468. Riboflavin_kinase. IPR002606. Riboflavin_kinase_bac. IPR015865. Riboflavin_kinase_bac/euk. IPR023465. Riboflavin_kinase_domain. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR22749. PTHR22749. 1 hit.
Pfam	PF06574. FAD_syn. 1 hit. PF01687. Flavokinase. 1 hit. [Graphical view]
PIRSF	PIRSF004491. FAD_Synth. 1 hit.
SMART	SM00904. Flavokinase. 1 hit. [Graphical view]
SUPFAM	SSF82114. Riboflavin_kinase. 1 hit.
TIGRFAMs	TIGR00083. ribF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBF_PSEFL

Accession

Primary (citable) accession number: P22990

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program