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Protein

L-lactate dehydrogenase B

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase B, L-lactate dehydrogenase A
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109NADBy similarity1
Binding sitei116SubstrateBy similarity1
Binding sitei148NAD or substrateBy similarity1
Binding sitei179SubstrateBy similarity1
Active sitei203Proton acceptorBy similarity1
Binding sitei258SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 67NADBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processStress response
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase B (EC:1.1.1.27)
Short name:
LDH-B
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000168490‹1 – 344L-lactate dehydrogenase BAdd BLAST›344

Expressioni

Inductioni

By hypoxia.

Interactioni

Subunit structurei

Tetramer that arise from random association of LDH-A and LDH-B.

Structurei

3D structure databases

ProteinModelPortaliP22989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.

Family and domain databases

Gene3Di3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiView protein in InterPro
IPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiView protein in PROSITE
PS00064. L_LDH. 1 hit.

Sequencei

Sequence statusi: Fragment.

P22989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AGDASSGFFR AVADGCPITH TSCSAPHRRL TKVSVIGAGN VGMAIAQTIL
60 70 80 90 100
TQNLADEIAL VDALPDKLRG EALDLQHAAA FLPRVRIVSG TDAAVTKNSD
110 120 130 140 150
LIVVTAGARQ IPGETRLNLL QRNVALYRKI VPPVAEHSPD ALLLVVSNPV
160 170 180 190 200
DVLTYVAWKL SGFPASRVIG SGTNLDSSRF RFLVAEHLDV SAQDVQAYMV
210 220 230 240 250
GEHGDSSVAI WSSISVGGMP ALKSLRDSHR SFDEAALEGI RRAVVGGAYE
260 270 280 290 300
VIGLKGYTSW AIGYSVASLA TSLLRDQRRV HPVSVLAAGF HGISDGHEVF
310 320 330 340
LSLPARLGRA GVLGVAEMDL TEAEAAQLRR SAKTLWENCQ LLGL
Length:344
Mass (Da):36,430
Last modified:August 1, 1991 - v1
Checksum:i21F9BFDA80C8B251
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55684 mRNA. Translation: AAA62697.1.
PIRiB36070.

Similar proteinsi

Entry informationi

Entry nameiLDHB_HORVU
AccessioniPrimary (citable) accession number: P22989
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 25, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families