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Protein

L-lactate dehydrogenase B

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase B, L-lactate dehydrogenase A
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109NADBy similarity1
Binding sitei116SubstrateBy similarity1
Binding sitei148NAD or substrateBy similarity1
Binding sitei179SubstrateBy similarity1
Active sitei203Proton acceptorBy similarity1
Binding sitei258SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 67NADBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processStress response
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase B (EC:1.1.1.27)
Short name:
LDH-B
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000168490‹1 – 344L-lactate dehydrogenase BAdd BLAST›344

Expressioni

Inductioni

By hypoxia.

Interactioni

Subunit structurei

Tetramer that arise from random association of LDH-A and LDH-B.

Structurei

3D structure databases

ProteinModelPortaliP22989
SMRiP22989
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495 Eukaryota
COG0039 LUCA

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Fragment.

P22989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AGDASSGFFR AVADGCPITH TSCSAPHRRL TKVSVIGAGN VGMAIAQTIL
60 70 80 90 100
TQNLADEIAL VDALPDKLRG EALDLQHAAA FLPRVRIVSG TDAAVTKNSD
110 120 130 140 150
LIVVTAGARQ IPGETRLNLL QRNVALYRKI VPPVAEHSPD ALLLVVSNPV
160 170 180 190 200
DVLTYVAWKL SGFPASRVIG SGTNLDSSRF RFLVAEHLDV SAQDVQAYMV
210 220 230 240 250
GEHGDSSVAI WSSISVGGMP ALKSLRDSHR SFDEAALEGI RRAVVGGAYE
260 270 280 290 300
VIGLKGYTSW AIGYSVASLA TSLLRDQRRV HPVSVLAAGF HGISDGHEVF
310 320 330 340
LSLPARLGRA GVLGVAEMDL TEAEAAQLRR SAKTLWENCQ LLGL
Length:344
Mass (Da):36,430
Last modified:August 1, 1991 - v1
Checksum:i21F9BFDA80C8B251
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55684 mRNA Translation: AAA62697.1
PIRiB36070

Similar proteinsi

Entry informationi

Entry nameiLDHB_HORVU
AccessioniPrimary (citable) accession number: P22989
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 25, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing
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Main funding by: National Institutes of Health