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Protein

L-lactate dehydrogenase A

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase B, L-lactate dehydrogenase A
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei121NADBy similarity1
Binding sitei128SubstrateBy similarity1
Binding sitei160NAD or substrateBy similarity1
Binding sitei191SubstrateBy similarity1
Active sitei215Proton acceptorBy similarity1
Binding sitei270SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 80NADBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processStress response
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase A (EC:1.1.1.27)
Short name:
LDH-A
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001684891 – 356L-lactate dehydrogenase AAdd BLAST356

Expressioni

Inductioni

By hypoxia.

Gene expression databases

ExpressionAtlasiP22988. differential.

Interactioni

Subunit structurei

Tetramer that arise from random association of LDH-A and LDH-B.

Structurei

3D structure databases

ProteinModelPortaliP22988.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.

Family and domain databases

Gene3Di3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiView protein in InterPro
IPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR036291. NAD(P)-bd_dom_sf.
PfamiView protein in Pfam
PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiView protein in PROSITE
PS00064. L_LDH. 1 hit.

Sequencei

Sequence statusi: Complete.

P22988-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKASSLSEL GFDAGGASSG FFRPVADGCP ATPTSSAVPH RRLTKISVIG
60 70 80 90 100
AGNVGMAIAQ TILTQNLADE IALVDALPDK LRGEALDLQH AAAFLPRVRI
110 120 130 140 150
SGTDAAVTKN SDLVIVTAGA RQIPGETRLN LLQRNVALYR KIVPPVAEHS
160 170 180 190 200
PDALLLVVSN PVDVLTYVAW KLSGFPASRV IGSGTNLDSS RFRFLIAEHL
210 220 230 240 250
DVNAQDVQAY MVGEHGDSSV AIWSSISVGG MPAFKSLRDS HRSFDEAALE
260 270 280 290 300
GIRRAVVGGA YEVIGLKGYT SWAIGYSVAS LAASLLRDQR RVHPVSVLAS
310 320 330 340 350
GFHGISDGHE VFLSLPARLG RGGILGVAEM DLTEAEAAQL RRSAKTLWEN

CQLLDL
Length:356
Mass (Da):37,749
Last modified:August 1, 1991 - v1
Checksum:i7B82D6F1D0BF5AD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55685 mRNA. Translation: AAA62696.1.
PIRiA36070.

Similar proteinsi

Entry informationi

Entry nameiLDHA_HORVU
AccessioniPrimary (citable) accession number: P22988
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: December 20, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families