P22988 (LDHA_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: L-lactate dehydrogenase A Short name=LDH-A EC=1.1.1.27
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Tetramer that arise from random association of LDH-A and LDH-B.
Induction	By hypoxia.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis Stress response
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	L-lactate dehydrogenase A		PRO_0000168489
Regions
	Nucleotide binding	75 – 80	6	NAD By similarity
Sites
	Active site	215	1	Proton acceptor By similarity
	Binding site	121	1	NAD By similarity
	Binding site	128	1	Substrate By similarity
	Binding site	160	1	NAD or substrate By similarity
	Binding site	191	1	Substrate By similarity
	Binding site	270	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22988 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 7B82D6F1D0BF5AD8	FASTA	356	37,749
	10 20 30 40 50 60 MHKASSLSEL GFDAGGASSG FFRPVADGCP ATPTSSAVPH RRLTKISVIG AGNVGMAIAQ 70 80 90 100 110 120 TILTQNLADE IALVDALPDK LRGEALDLQH AAAFLPRVRI SGTDAAVTKN SDLVIVTAGA 130 140 150 160 170 180 RQIPGETRLN LLQRNVALYR KIVPPVAEHS PDALLLVVSN PVDVLTYVAW KLSGFPASRV 190 200 210 220 230 240 IGSGTNLDSS RFRFLIAEHL DVNAQDVQAY MVGEHGDSSV AIWSSISVGG MPAFKSLRDS 250 260 270 280 290 300 HRSFDEAALE GIRRAVVGGA YEVIGLKGYT SWAIGYSVAS LAASLLRDQR RVHPVSVLAS 310 320 330 340 350 GFHGISDGHE VFLSLPARLG RGGILGVAEM DLTEAEAAQL RRSAKTLWEN CQLLDL

References

[1]	"Hypoxically inducible barley lactate dehydrogenase: cDNA cloning and molecular analysis." Hondred D., Hanson A.D. Proc. Natl. Acad. Sci. U.S.A. 87:7300-7304(1990) [PubMed: 1698294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Himalaya. Tissue: Root.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55685 mRNA. Translation: AAA62696.1.
PIR	A36070.
3D structure databases
ProteinModelPortal	P22988.
SMR	P22988. Positions 44-356.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P22988.
Gene expression databases
Genevestigator	P22988.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDHA_HORVU

Accession

Primary (citable) accession number: P22988

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	December 14, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program