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Protein

Ras protein let-60

Gene

let-60

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The level of let-60 controls the switch between vulval and hypodermal cell fates during C.elegans vulval induction (PubMed:2257629, PubMed:2123303, PubMed:24929033). May stimulate the guanine nucleotide exchange factor (GEF) activity of rap-1 (PubMed:10608844).4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi116 – 1194GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: WormBase
  • GTP binding Source: WormBase
  • phospholipase binding Source: WormBase
  • protein kinase binding Source: WormBase
  • Rap guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  • associative learning Source: WormBase
  • epidermal growth factor receptor signaling pathway Source: WormBase
  • muscle organ development Source: WormBase
  • nematode larval development Source: WormBase
  • oogenesis Source: WormBase
  • positive regulation of GTPase activity Source: UniProtKB
  • positive regulation of receptor localization to synapse Source: WormBase
  • positive regulation of vulval development Source: WormBase
  • regulation of cell fate specification Source: WormBase
  • regulation of cell projection organization Source: WormBase
  • reproduction Source: WormBase
  • short-term memory Source: WormBase
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-1169092. Activation of RAS in B cells.
R-CEL-171007. p38MAPK events.
R-CEL-179812. GRB2 events in EGFR signaling.
R-CEL-180336. SHC1 events in EGFR signaling.
R-CEL-210993. Tie2 Signaling.
R-CEL-2179392. EGFR Transactivation by Gastrin.
R-CEL-2424491. DAP12 signaling.
R-CEL-2871796. FCERI mediated MAPK activation.
R-CEL-375165. NCAM signaling for neurite out-growth.
R-CEL-3928662. EPHB-mediated forward signaling.
R-CEL-5621575. CD209 (DC-SIGN) signaling.
R-CEL-5654688. SHC-mediated cascade:FGFR1.
R-CEL-5654693. FRS-mediated FGFR1 signaling.
R-CEL-5654699. SHC-mediated cascade:FGFR2.
R-CEL-5654700. FRS-mediated FGFR2 signaling.
R-CEL-5654704. SHC-mediated cascade:FGFR3.
R-CEL-5654706. FRS-mediated FGFR3 signaling.
R-CEL-5654712. FRS-mediated FGFR4 signaling.
R-CEL-5654719. SHC-mediated cascade:FGFR4.
R-CEL-5658442. Regulation of RAS by GAPs.
R-CEL-5673000. RAF activation.
R-CEL-5673001. RAF/MAP kinase cascade.
R-CEL-5674135. MAP2K and MAPK activation.
R-CEL-5675221. Negative regulation of MAPK pathway.
SignaLinkiP22981.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras protein let-60
Alternative name(s):
Abnormal cell lineage protein 34
Lethal protein 60
Gene namesi
Name:let-60
Synonyms:lin-34
ORF Names:ZK792.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiZK792.6; CE03827; WBGene00002335; let-60.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: WormBase
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi13 – 131G → E in n1046; multivulva phenotype. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181Ras protein let-60PRO_0000082686Add
BLAST
Propeptidei182 – 1843Removed in mature formBy similarityPRO_0000281335

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811Cysteine methyl esterBy similarity
Lipidationi181 – 1811S-farnesyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

EPDiP22981.
PaxDbiP22981.
PRIDEiP22981.

Expressioni

Tissue specificityi

Expressed in body wall muscles and in the nervous system including ganglion, nerve ring dorsal and ventral nerve cords, motor neurons and sensory tail neurons.1 Publication

Interactioni

Subunit structurei

Interacts with soc-2.1 Publication

GO - Molecular functioni

  • phospholipase binding Source: WormBase
  • protein kinase binding Source: WormBase

Protein-protein interaction databases

BioGridi43199. 134 interactions.
DIPiDIP-25369N.
IntActiP22981. 6 interactions.
MINTiMINT-115792.
STRINGi6239.ZK792.6.

Structurei

3D structure databases

ProteinModelPortaliP22981.
SMRiP22981. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector region

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121849.
HOGENOMiHOG000233973.
InParanoidiP22981.
KOiK07827.
OMAiDSGPGCM.
OrthoDBiEOG7QVM41.
PhylomeDBiP22981.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22981-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYKLVVVG DGGVGKSALT IQLIQNHFVE EYDPTIEDSY RKQVVIDGET
60 70 80 90 100
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLL VFAVNEAKSF ENVANYREQI
110 120 130 140 150
RRVKDSDDVP MVLVGNKCDL SSRSVDFRTV SETAKGYGIP NVDTSAKTRM
160 170 180
GVDEAFYTLV REIRKHRERH DNNKPQKKKK CQIM
Length:184
Mass (Da):20,983
Last modified:August 1, 1991 - v1
Checksum:iD32BA6B67DAE14F9
GO

Sequence cautioni

The sequence AAA28103.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55535 mRNA. Translation: AAA28103.1. Different initiation.
Z68302 Genomic DNA. Translation: CAA92630.1.
PIRiA36290.
RefSeqiNP_502213.3. NM_069812.3.
UniGeneiCel.19758.

Genome annotation databases

EnsemblMetazoaiZK792.6; ZK792.6; WBGene00002335.
GeneIDi178104.
KEGGicel:CELE_ZK792.6.
UCSCiZK792.6. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55535 mRNA. Translation: AAA28103.1. Different initiation.
Z68302 Genomic DNA. Translation: CAA92630.1.
PIRiA36290.
RefSeqiNP_502213.3. NM_069812.3.
UniGeneiCel.19758.

3D structure databases

ProteinModelPortaliP22981.
SMRiP22981. Positions 1-166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi43199. 134 interactions.
DIPiDIP-25369N.
IntActiP22981. 6 interactions.
MINTiMINT-115792.
STRINGi6239.ZK792.6.

Proteomic databases

EPDiP22981.
PaxDbiP22981.
PRIDEiP22981.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK792.6; ZK792.6; WBGene00002335.
GeneIDi178104.
KEGGicel:CELE_ZK792.6.
UCSCiZK792.6. c. elegans.

Organism-specific databases

CTDi178104.
WormBaseiZK792.6; CE03827; WBGene00002335; let-60.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121849.
HOGENOMiHOG000233973.
InParanoidiP22981.
KOiK07827.
OMAiDSGPGCM.
OrthoDBiEOG7QVM41.
PhylomeDBiP22981.

Enzyme and pathway databases

ReactomeiR-CEL-1169092. Activation of RAS in B cells.
R-CEL-171007. p38MAPK events.
R-CEL-179812. GRB2 events in EGFR signaling.
R-CEL-180336. SHC1 events in EGFR signaling.
R-CEL-210993. Tie2 Signaling.
R-CEL-2179392. EGFR Transactivation by Gastrin.
R-CEL-2424491. DAP12 signaling.
R-CEL-2871796. FCERI mediated MAPK activation.
R-CEL-375165. NCAM signaling for neurite out-growth.
R-CEL-3928662. EPHB-mediated forward signaling.
R-CEL-5621575. CD209 (DC-SIGN) signaling.
R-CEL-5654688. SHC-mediated cascade:FGFR1.
R-CEL-5654693. FRS-mediated FGFR1 signaling.
R-CEL-5654699. SHC-mediated cascade:FGFR2.
R-CEL-5654700. FRS-mediated FGFR2 signaling.
R-CEL-5654704. SHC-mediated cascade:FGFR3.
R-CEL-5654706. FRS-mediated FGFR3 signaling.
R-CEL-5654712. FRS-mediated FGFR4 signaling.
R-CEL-5654719. SHC-mediated cascade:FGFR4.
R-CEL-5658442. Regulation of RAS by GAPs.
R-CEL-5673000. RAF activation.
R-CEL-5673001. RAF/MAP kinase cascade.
R-CEL-5674135. MAP2K and MAPK activation.
R-CEL-5675221. Negative regulation of MAPK pathway.
SignaLinkiP22981.

Miscellaneous databases

NextBioi899728.
PROiP22981.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "let-60, a gene that specifies cell fates during C. elegans vulval induction, encodes a ras protein."
    Han M., Sternberg P.W.
    Cell 63:921-931(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Caenorhabditis elegans ras gene let-60 acts as a switch in the pathway of vulval induction."
    Beitel G.J., Clark S.C., Horvitz H.R.
    Nature 348:503-509(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLY-13.
  4. "SUR-8, a conserved Ras-binding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C. elegans."
    Sieburth D.S., Sun Q., Han M.
    Cell 94:119-130(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SOC-2.
  5. "RA-GEF, a novel Rap1A guanine nucleotide exchange factor containing a Ras/Rap1A-associating domain, is conserved between nematode and humans."
    Liao Y., Kariya K., Hu C.-D., Shibatohge M., Goshima M., Okada T., Watari Y., Gao X., Jin T.-G., Yamawaki-Kataoka Y., Kataoka T.
    J. Biol. Chem. 274:37815-37820(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION IN GEF ACTIVITY.
  6. "Expression pattern and first functional characterization of riok-1 in Caenorhabditis elegans."
    Weinberg F., Schulze E., Fatouros C., Schmidt E., Baumeister R., Brummer T.
    Gene Expr. Patterns 15:124-134(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF GLY-13.

Entry informationi

Entry nameiLET60_CAEEL
AccessioniPrimary (citable) accession number: P22981
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: May 11, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.