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Protein

RNA-directed RNA polymerase

Gene

ORF1

Organism
Tobacco necrosis virus (strain A) (TNV-A)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

RNA-dependent RNA polymerase that plays an essential role in the virus replication.Curated

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological processViral RNA replication
LigandNucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein p82
Cleaved into the following chain:
Gene namesi
ORF Names:ORF1
OrganismiTobacco necrosis virus (strain A) (TNV-A)
Taxonomic identifieri12055 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageTombusviridaeAlphanecrovirus
Virus hostiChenopodium amaranticolor [TaxID: 66262]
Chenopodium quinoa (Quinoa) [TaxID: 63459]
Cucumis sativus (Cucumber) [TaxID: 3659]
Nicotiana clevelandii (Wild tobacco) [TaxID: 81866]
Nicotiana tabacum (Common tobacco) [TaxID: 4097]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Tulipa gesneriana (Garden tulip) [TaxID: 13306]
Proteomesi
  • UP000000575 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000402171 – 724RNA-directed RNA polymeraseAdd BLAST724
ChainiPRO_00000402181 – 202Protein p23Add BLAST202

Structurei

3D structure databases

ProteinModelPortaliP22958.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini424 – 540RdRp catalyticPROSITE-ProRule annotationAdd BLAST117

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG090B0008.

Family and domain databases

InterProiView protein in InterPro
IPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR013707. Tombusvirus_p33.
PfamiView protein in Pfam
PF00998. RdRP_3. 1 hit.
PF08500. Tombus_P33. 1 hit.
PROSITEiView protein in PROSITE
PS50507. RDRP_SSRNA_POS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELPNQHKQT AAEGFVSFLN WLCNPWRRQR TVNAAVAFQK DLLAIEDSEH
60 70 80 90 100
LDDINECFEE SAGAQSQRTK VVADGAYAPA KSNRTRRVRK QKKHKFVKYL
110 120 130 140 150
VNEARAEFGL PKPTEANRLM VQHFLLRVCK DWGVVTAHVH GNVALALPLV
160 170 180 190 200
FIPTEDDLLS RALMNTHATR AAVRGMDNVQ GEGWWNNRLG IGGQVGLAFR
210 220 230 240 250
SKXGCLERRP GFSTSVSRGE HPDLVVIPSG RPEKQRQLLR YSGIGGHLLI
260 270 280 290 300
GIHNNSLSNL RRGLMERVFY VEGPNGLQDA PKPVKGAFRT LDKFRDLYTK
310 320 330 340 350
NSWRHTPVTS EQFLMNYTGR KLTIYREAVD SLSHQPLSSR DAKLKTFVKA
360 370 380 390 400
EKLNLSKKPD PAPRVIQPRS PRYNVCLGRY LRHYEHHAFK TIAKCFGEIT
410 420 430 440 450
VFKGFTLEQQ GEIMRSKWNK YVNPVAVGLD ASRFDQHVSV EALEYEHEFY
460 470 480 490 500
LRDYPNDKQL KWLLKQQLCN VGTAFASDGI IKYKKKGCRM SGDMNTSLGN
510 520 530 540 550
CILMCAMVYG LKEHLNINLS LANNGDDCVI VCEKADLKKL TSSIEPYFKQ
560 570 580 590 600
FGFKMEVEKP VDIFERIEFC QTQPVFDGSQ YIMVRKPSVV TSKDVTSLIP
610 620 630 640 650
CQTKAQYAEW LQAVGECGMS INGGIPVMQN FYQKLQTGIR RTKFTKTGEF
660 670 680 690 700
QTNGLGYHSR YMHRVARVPS PETRLSFYLA FGITPDLQEA LEIFYDTHKL
710 720
ELDDVIPTDT YQVSGEHLIN GLPN
Length:724
Mass (Da):82,279
Last modified:October 5, 2010 - v2
Checksum:i87E6761AA1BA2164
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti72V → A. 1
Natural varianti699K → R. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33002 Genomic RNA. Translation: AAA86434.2.
PIRiA35523. RRWQTN.
RefSeqiNP_056825.2. NC_001777.1.

Genome annotation databases

GeneIDi1493899.
KEGGivg:1493899.

Keywords - Coding sequence diversityi

RNA suppression of termination

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33002 Genomic RNA. Translation: AAA86434.2.
PIRiA35523. RRWQTN.
RefSeqiNP_056825.2. NC_001777.1.

3D structure databases

ProteinModelPortaliP22958.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1493899.
KEGGivg:1493899.

Phylogenomic databases

OrthoDBiVOG090B0008.

Family and domain databases

InterProiView protein in InterPro
IPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR013707. Tombusvirus_p33.
PfamiView protein in Pfam
PF00998. RdRP_3. 1 hit.
PF08500. Tombus_P33. 1 hit.
PROSITEiView protein in PROSITE
PS50507. RDRP_SSRNA_POS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRDRP_TNVA
AccessioniPrimary (citable) accession number: P22958
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 5, 2010
Last modified: February 15, 2017
This is version 74 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Readthrough of the terminator UAG occurs at position 203.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.