Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA-directed RNA polymerase

Gene

ORF1

Organism
Tobacco necrosis virus (strain A) (TNV-A)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

RNA-dependent RNA polymerase that plays an essential role in the virus replication.Curated

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein p82
Cleaved into the following chain:
Gene namesi
ORF Names:ORF1
OrganismiTobacco necrosis virus (strain A) (TNV-A)
Taxonomic identifieri12055 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageTombusviridaeAlphanecrovirus
Virus hostiChenopodium amaranticolor [TaxID: 66262]
Chenopodium quinoa (Quinoa) [TaxID: 63459]
Cucumis sativus (Cucumber) [TaxID: 3659]
Nicotiana clevelandii (Wild tobacco) [TaxID: 81866]
Nicotiana tabacum (Common tobacco) [TaxID: 4097]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Tulipa gesneriana (Garden tulip) [TaxID: 13306]
ProteomesiUP000000575: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 724724RNA-directed RNA polymerasePRO_0000040217Add
BLAST
Chaini1 – 202202Protein p23PRO_0000040218Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP22958.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini424 – 540117RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR013707. Tombusvirus_p33.
[Graphical view]
PfamiPF00998. RdRP_3. 1 hit.
PF08500. Tombus_P33. 1 hit.
[Graphical view]
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELPNQHKQT AAEGFVSFLN WLCNPWRRQR TVNAAVAFQK DLLAIEDSEH
60 70 80 90 100
LDDINECFEE SAGAQSQRTK VVADGAYAPA KSNRTRRVRK QKKHKFVKYL
110 120 130 140 150
VNEARAEFGL PKPTEANRLM VQHFLLRVCK DWGVVTAHVH GNVALALPLV
160 170 180 190 200
FIPTEDDLLS RALMNTHATR AAVRGMDNVQ GEGWWNNRLG IGGQVGLAFR
210 220 230 240 250
SKXGCLERRP GFSTSVSRGE HPDLVVIPSG RPEKQRQLLR YSGIGGHLLI
260 270 280 290 300
GIHNNSLSNL RRGLMERVFY VEGPNGLQDA PKPVKGAFRT LDKFRDLYTK
310 320 330 340 350
NSWRHTPVTS EQFLMNYTGR KLTIYREAVD SLSHQPLSSR DAKLKTFVKA
360 370 380 390 400
EKLNLSKKPD PAPRVIQPRS PRYNVCLGRY LRHYEHHAFK TIAKCFGEIT
410 420 430 440 450
VFKGFTLEQQ GEIMRSKWNK YVNPVAVGLD ASRFDQHVSV EALEYEHEFY
460 470 480 490 500
LRDYPNDKQL KWLLKQQLCN VGTAFASDGI IKYKKKGCRM SGDMNTSLGN
510 520 530 540 550
CILMCAMVYG LKEHLNINLS LANNGDDCVI VCEKADLKKL TSSIEPYFKQ
560 570 580 590 600
FGFKMEVEKP VDIFERIEFC QTQPVFDGSQ YIMVRKPSVV TSKDVTSLIP
610 620 630 640 650
CQTKAQYAEW LQAVGECGMS INGGIPVMQN FYQKLQTGIR RTKFTKTGEF
660 670 680 690 700
QTNGLGYHSR YMHRVARVPS PETRLSFYLA FGITPDLQEA LEIFYDTHKL
710 720
ELDDVIPTDT YQVSGEHLIN GLPN
Length:724
Mass (Da):82,279
Last modified:October 5, 2010 - v2
Checksum:i87E6761AA1BA2164
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti72 – 721V → A.
Natural varianti699 – 6991K → R.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33002 Genomic RNA. Translation: AAA86434.2.
PIRiA35523. RRWQTN.
RefSeqiNP_056825.2. NC_001777.1.

Genome annotation databases

GeneIDi1493899.

Keywords - Coding sequence diversityi

RNA suppression of termination

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33002 Genomic RNA. Translation: AAA86434.2.
PIRiA35523. RRWQTN.
RefSeqiNP_056825.2. NC_001777.1.

3D structure databases

ProteinModelPortaliP22958.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1493899.

Family and domain databases

InterProiIPR007094. RNA-dir_pol_PSvirus.
IPR002166. RNA_pol_HCV.
IPR013707. Tombusvirus_p33.
[Graphical view]
PfamiPF00998. RdRP_3. 1 hit.
PF08500. Tombus_P33. 1 hit.
[Graphical view]
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome structure of tobacco necrosis virus strain A."
    Meulewaeter F., Seurinck J., van Emmelo J.
    Virology 177:699-709(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiRDRP_TNVA
AccessioniPrimary (citable) accession number: P22958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 5, 2010
Last modified: January 7, 2015
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Readthrough of the terminator UAG occurs at position 203.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.