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P22956

- RDRP_RCNMV

UniProt

P22956 - RDRP_RCNMV

Protein

RNA-directed RNA polymerase

Gene

ORF1

Organism
Red clover necrotic mosaic virus (RCNMV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 2 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    RNA-dependent RNA polymerase that plays an essential role in the virus replication.1 Publication

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA binding Source: InterPro
    3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Alternative name(s):
    Protein p88
    Gene namesi
    ORF Names:ORF1
    OrganismiRed clover necrotic mosaic virus (RCNMV)
    Taxonomic identifieri12267 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageTombusviridaeDianthovirus
    Virus hostiMedicago sativa (Alfalfa) [TaxID: 3879]
    Melilotus officinalis (Yellow sweet clover) (Trifolium officinale) [TaxID: 47083]
    Trifolium pratense (Red clover) [TaxID: 57577]
    Trifolium repens (Creeping white clover) [TaxID: 3899]
    ProteomesiUP000008651: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 767767RNA-directed RNA polymerasePRO_0000040206Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP22956.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini463 – 580118RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR007094. RNA-dir_pol_PSvirus.
    IPR002166. RNA_pol_HCV.
    [Graphical view]
    PfamiPF00998. RdRP_3. 1 hit.
    [Graphical view]
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Isoform RNA-directed RNA polymerase p88 (identifier: P22956-1) [UniParc]FASTAAdd to Basket

    Also known as: 88 kDa protein

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGFINLSLFD VDKLMVWVSK FNPGKILSAI CNLGIDCWNR FRKWFFGLNF    50
    DAHMWAVDAF IPLMPHYTEQ MERVVDDFCS ETPESKLEDC LELDTSVNEF 100
    FDEEVYKKDE EGVMKLQRSA ARKHIKRVRP GMMQAAIKAV ETRIRNRHTI 150
    FGDDMGKVDE AAVRATASDI CGEFKINEHH TNALVYAAAY LAMTPDQRSI 200
    DSVKLAYNPK SQARRTLVSA IRENKAVAGF KSLEDFLGGP LSFPVEDAPY 250
    PILGIPEIRV AEKRASRVMK SKRVVGLPAV SAGLKVCVHQ TSLHNMIVSL 300
    ERRVFRVKNS AGELVVPPKP IQNAFDSISY FREEWLRKLS HKGQILKSSL 350
    ADVVACYSSE KRKLYQKAAD SLEKKPVQWR DSKVQAFIKV EKLECDTKDP 400
    VPRTIQPRSK RYNLAIGQYL RLNEKKMLDS IDDVFKEKTV LSGLDNRAQG 450
    RAIAHKWRKY QNPIGIGLDA SRFDQHCSVD ALKFEQTFYK ACFPGDQQLE 500
    TLLKWQLSNT GSALLPTGEL VRYRTKGCRM SGDINTGLGN KILMCSMVHA 550
    FLKETGVRAS LANNGDDCVL FCEKGDYEQI NRNLEQWFLC RGFEMTVEKP 600
    VDVLEKVAFC RSQPVCIATQ WAMVRQLGSL SRDCFSTQDW LNPKTFKDAM 650
    NALGQCNGII NDGVPIHMAQ AKLMHRIGGN RKFNLDALHK QMEYSWRDRL 700
    GKRTNLLWSE VEDATRLSYF RAFGIEPYIQ RIVEEYFSQV EITCEGRSTN 750
    VLPTHYSRIH KDLIKAR 767

    Note: Produced by ribosomal frameshifting between codons 236 and 237.

    Length:767
    Mass (Da):87,544
    Last modified:November 1, 1991 - v2
    Checksum:i3515C03BFF4F7A1B
    GO
    Isoform protein p27 (identifier: P22956-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         237-767: Missing.

    Note: Produced by conventional translation.

    Show »
    Length:236
    Mass (Da):27,015
    Checksum:i44AD4823A36CB73B
    GO

    Sequence cautioni

    The sequence AAB02541.1 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti237 – 2371Missing in AAB02540. (PubMed:2763465)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei237 – 767531Missing in isoform protein p27. CuratedVSP_032886Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04357 Genomic RNA. Translation: AAB02539.1.
    J04357 Genomic RNA. Translation: AAB02540.1.
    J04357 Genomic RNA. Translation: AAB02541.1. Sequence problems.
    PIRiT10779. B43684.
    RefSeqiNP_620523.1. NC_003756.1.
    NP_620524.1. NC_003756.1. [P22956-2]

    Genome annotation databases

    GeneIDi956630.
    956631.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04357 Genomic RNA. Translation: AAB02539.1 .
    J04357 Genomic RNA. Translation: AAB02540.1 .
    J04357 Genomic RNA. Translation: AAB02541.1 . Sequence problems.
    PIRi T10779. B43684.
    RefSeqi NP_620523.1. NC_003756.1.
    NP_620524.1. NC_003756.1. [P22956-2 ]

    3D structure databases

    ProteinModelPortali P22956.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956630.
    956631.

    Family and domain databases

    InterProi IPR007094. RNA-dir_pol_PSvirus.
    IPR002166. RNA_pol_HCV.
    [Graphical view ]
    Pfami PF00998. RdRP_3. 1 hit.
    [Graphical view ]
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete nucleotide sequence and genome organization of red clover necrotic mosaic virus RNA-1."
      Xiong Z., Lommel S.A.
      Virology 171:543-554(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Australian.
    2. "Synthesis of the putative red clover necrotic mosaic virus RNA polymerase by ribosomal frameshifting in vitro."
      Xiong Z., Kim K.H., Kendall T.L., Lommel S.A.
      Virology 193:213-221(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: RIBOSOMAL FRAMESHIFT.
    3. "A plant RNA virus suppresses RNA silencing through viral RNA replication."
      Takeda A., Tsukuda M., Mizumoto H., Okamoto K., Kaido M., Mise K., Okuno T.
      EMBO J. 24:3147-3157(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiRDRP_RCNMV
    AccessioniPrimary (citable) accession number: P22956
    Secondary accession number(s): P22957, Q86156, Q86157
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 74 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3