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Protein

Probable mediator of RNA polymerase II transcription subunit 37c

Gene

MED37D

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to bacterium Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to heat Source: UniProtKB
  • response to virus Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChaperone
Biological processStress response, Transcription, Transcription regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371497. HSP90 chaperone cycle for steroid hormone receptors (SHR).
R-ATH-3371568. Attenuation phase.
R-ATH-3371571. HSF1-dependent transactivation.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-72163. mRNA Splicing - Major Pathway.
R-ATH-8876725. Protein methylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mediator of RNA polymerase II transcription subunit 37c
Alternative name(s):
Heat shock 70 kDa protein 2
Heat shock cognate 70 kDa protein 2
Heat shock cognate protein 70-2
Short name:
AtHsc70-2
Heat shock protein 70-2
Short name:
AtHsp70-2
Gene namesi
Name:MED37D
Synonyms:HSC70-2, HSC70-G8, HSP70-2, MED37_3
Ordered Locus Names:At5g02490
ORF Names:T22P11.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G02490.
TAIRilocus:2181818. AT5G02490.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000783451 – 653Probable mediator of RNA polymerase II transcription subunit 37cAdd BLAST653

Proteomic databases

PaxDbiP22954.
PRIDEiP22954.

PTM databases

iPTMnetiP22954.

Expressioni

Developmental stagei

Down-regulated during seed maturation.1 Publication

Inductioni

By heat shock and by cold. Up-regulated by virus infection and by pathogen attack (P.syringae).3 Publications

Gene expression databases

ExpressionAtlasiP22954. baseline and differential.
GenevisibleiP22954. AT.

Interactioni

Subunit structurei

Component of the Mediator complex.1 Publication

Protein-protein interaction databases

BioGridi17132. 13 interactors.
IntActiP22954. 2 interactors.
STRINGi3702.AT5G02490.1.

Structurei

3D structure databases

ProteinModelPortaliP22954.
SMRiP22954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228135.
InParanoidiP22954.
KOiK03283.
OMAiWPFTIIS.
OrthoDBiEOG093604KP.
PhylomeDBiP22954.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiView protein in InterPro
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
PfamiView protein in Pfam
PF00012. HSP70. 1 hit.
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiView protein in PROSITE
PS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P22954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKGEGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS
60 70 80 90 100
ERLIGDAAKN QVAMNPVNTV FDAKRLIGRR FSDASVQSDR QLWPFTIISG
110 120 130 140 150
TAEKPMIVVE YKGEEKQFAA EEISSMVLIK MREIAEAFLG TTVKNAVVTV
160 170 180 190 200
PAYFNDSQRQ ATKDAGVIAG LNVLRIINEP TAAAIAYGLD KKATSVGEKN
210 220 230 240 250
VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRMVNHFVQE
260 270 280 290 300
FKRKNKQDIT GQPRALRRLR TACERAKRTL SSTAQTTIEI DSLYGGADFY
310 320 330 340 350
SPITRARFEE MNMDLFRKCM EPVEKCLRDA KMDKSTVHEI VLVGGSTRIP
360 370 380 390 400
KVQQLLQDFF NGKELCKSIN PDEAVAYGAA VQAAILSGEG NEKVQDLLLL
410 420 430 440 450
DVTPLSLGLE TAGGVMTTLI QRNTTIPTKK EQVFSTYSDN QPGVLIQVFE
460 470 480 490 500
GERARTKDNN LLGKFELSGI PPAPRGVPQI TVCFDIDANG ILNVSAEDKT
510 520 530 540 550
TGKKNKITIT NDKGRLSKED IEKMVQEAEK YKSEDEEHKK KVEAKNALEN
560 570 580 590 600
YAYNMRNTIR DEKIGEKLPA ADKKKVEDSI EEAIQWLDGN QLGEADEFED
610 620 630 640 650
KMKELESVCN PIIAKMYQGG AGGEAGGPGA SGMDEDEAPP ASGGAGPKIE

EVD
Length:653
Mass (Da):71,387
Last modified:December 1, 2000 - v2
Checksum:iEDEC254DB06D9966
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6E → K in CAA54420 (Ref. 6) Curated1
Sequence conflicti100 – 102GTA → ELQ in AAA32820 (PubMed:16666375).Curated3
Sequence conflicti614A → G in BAD94888 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL162971 Genomic DNA. Translation: CAB85986.1.
CP002688 Genomic DNA. Translation: AED90479.1.
AY093152 mRNA. Translation: AAM13151.1.
BT008411 mRNA. Translation: AAP37770.1.
AK222068 mRNA. Translation: BAD94888.1.
AK227090 mRNA. Translation: BAE99142.1.
AH001335 Genomic DNA. Translation: AAA32820.1.
X77199 Genomic DNA. Translation: CAA54420.1.
Y08892 mRNA. Translation: CAA70105.1.
PIRiJA0170.
T48270.
RefSeqiNP_195869.1. NM_120327.5.
UniGeneiAt.5363.
At.74798.

Genome annotation databases

EnsemblPlantsiAT5G02490.1; AT5G02490.1; AT5G02490.
GeneIDi831856.
GrameneiAT5G02490.1; AT5G02490.1; AT5G02490.
KEGGiath:AT5G02490.

Similar proteinsi

Entry informationi

Entry nameiMD37D_ARATH
AccessioniPrimary (citable) accession number: P22954
Secondary accession number(s): O04293
, Q0WUQ6, Q56WH2, Q9LZ53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: December 1, 2000
Last modified: September 27, 2017
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families