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Protein

Probable mediator of RNA polymerase II transcription subunit 37e

Gene

MED37E

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity
Heat shock protein probably involved in defense response. Chaperone involved in protein targeting to chloroplasts. May cooperate with SGT1 and HSP90 in R gene-mediated resistance towards the oomycete H.parasitica (downy mildew). Plays a role with WPP-domain proteins in facilitating WIT1 nuclear envelope targeting.3 Publications
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage (By similarity).By similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protease binding Source: UniProtKB

GO - Biological processi

  • defense response to bacterium Source: UniProtKB
  • defense response to fungus Source: UniProtKB
  • defense response to other organism Source: TAIR
  • negative regulation of seed germination Source: TAIR
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
  • response to heat Source: UniProtKB
  • response to virus Source: TAIR
  • stomatal closure Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Plant defense, Stress response, Transcription, Transcription regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-225-MONOMER.
ARA:GQT-44-MONOMER.
ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371568. Attenuation phase.
R-ATH-3371571. HSF1-dependent transactivation.
R-ATH-72163. mRNA Splicing - Major Pathway.

Protein family/group databases

TCDBi1.A.33.1.1. the cation channel-forming heat shock protein-70 (hsp70) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mediator of RNA polymerase II transcription subunit 37e
Alternative name(s):
Heat shock 70 kDa protein 1
Heat shock cognate 70 kDa protein 1
Heat shock cognate protein 70-1
Short name:
AtHsc70-1
Heat shock protein 70-1
Short name:
AtHsp70-1
Protein EARLY-RESPONSIVE TO DEHYDRATION 2
Gene namesi
Name:MED37E
Synonyms:ERD2, HSC70-1, HSP70-1, MED37_4
Ordered Locus Names:At5g02500
ORF Names:T22P11.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G02500.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • cytoplasm Source: UniProtKB
  • cytosol Source: TAIR
  • cytosolic ribosome Source: TAIR
  • Golgi apparatus Source: TAIR
  • membrane Source: TAIR
  • nucleolus Source: TAIR
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 651650Probable mediator of RNA polymerase II transcription subunit 37ePRO_0000078344Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Cross-linki457 – 457Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP22953.
PRIDEiP22953.

2D gel databases

SWISS-2DPAGEP22953.
World-2DPAGE0003:P22953.

PTM databases

iPTMnetiP22953.

Expressioni

Tissue specificityi

Expressed at a substantial level during normal growth in root, stem, leaf and flower tissues, but not in siliques.1 Publication

Developmental stagei

Down-regulated during seed maturation. Up-regulated during germination.1 Publication

Inductioni

By heat shock, cold, dehydration stress and abscisic acid (ABA). Up-regulated by virus infection.4 Publications

Gene expression databases

ExpressionAtlasiP22953. baseline and differential.
GenevisibleiP22953. AT.

Interactioni

Subunit structurei

Binds to the deubiquitinating enzyme AMSH3. Interacts with SGT1B (via SGS domain). Interacts with OEP61. Interacts with HSFA1A/HSF1. Interacts with BAG3, BAG4 and BAG5. Interacts with WPP1, WPP2 and WIT1. Component of a ternary complex composed of WPP1, HSP70-1 and WIT1. Component of the Mediator complex (Probable). Binds to TIR.Curated9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SGT1BQ9SUT53EBI-1238845,EBI-1581364

GO - Molecular functioni

  • protease binding Source: UniProtKB

Protein-protein interaction databases

BioGridi16298. 28 interactions.
IntActiP22953. 9 interactions.
MINTiMINT-8061914.
STRINGi3702.AT5G02500.1.

Structurei

3D structure databases

ProteinModelPortaliP22953.
SMRiP22953. Positions 6-619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228135.
InParanoidiP22953.
KOiK03283.
OMAiPMIYVEY.
OrthoDBiEOG093604KP.
PhylomeDBiP22953.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P22953-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGKGEGPAI GIDLGTTYSC VGVWQHDRVE IIANDQGNRT TPSYVAFTDS
60 70 80 90 100
ERLIGDAAKN QVAMNPVNTV FDAKRLIGRR FSDSSVQSDM KLWPFKIQAG
110 120 130 140 150
PADKPMIYVE YKGEEKEFAA EEISSMVLIK MREIAEAYLG VTIKNAVVTV
160 170 180 190 200
PAYFNDSQRQ ATKDAGVIAG LNVMRIINEP TAAAIAYGLD KKATSVGEKN
210 220 230 240 250
VLIFDLGGGT FDVSLLTIEE GIFEVKATAG DTHLGGEDFD NRMVNHFVQE
260 270 280 290 300
FKRKSKKDIT GNPRALRRLR TSCERAKRTL SSTAQTTIEI DSLYEGIDFY
310 320 330 340 350
STITRARFEE LNMDLFRKCM EPVEKCLRDA KMDKSTVHDV VLVGGSTRIP
360 370 380 390 400
KVQQLLQDFF NGKELCKSIN PDEAVAYGAA VQGAILSGEG NEKVQDLLLL
410 420 430 440 450
DVTPLSLGLE TAGGVMTTLI PRNTTIPTKK EQVFSTYSDN QPGVLIQVYE
460 470 480 490 500
GERARTKDNN LLGKFELSGI PPAPRGVPQI TVCFDIDANG ILNVSAEDKT
510 520 530 540 550
TGQKNKITIT NDKGRLSKDE IEKMVQEAEK YKSEDEEHKK KVEAKNALEN
560 570 580 590 600
YAYNMRNTIQ DEKIGEKLPA ADKKKIEDSI EQAIQWLEGN QLAEADEFED
610 620 630 640 650
KMKELESICN PIIAKMYQGA GGEAGGPGAS GMDDDAPPAS GGAGPKIEEV

D
Length:651
Mass (Da):71,358
Last modified:December 1, 2000 - v3
Checksum:iBD689BA0C936A420
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651N → S in CAA54419 (Ref. 6) Curated
Sequence conflicti195 – 1951S → R in CAA54419 (Ref. 6) Curated
Sequence conflicti195 – 1951S → T in CAA52684 (PubMed:11130714).Curated
Sequence conflicti372 – 3721D → V in CAA54419 (Ref. 6) Curated
Sequence conflicti376 – 3761A → V in CAA54419 (Ref. 6) Curated
Sequence conflicti507 – 5071I → V in CAA54419 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74604 mRNA. Translation: CAA52684.1.
AL162971 Genomic DNA. Translation: CAB85987.1.
CP002688 Genomic DNA. Translation: AED90480.1.
AY035123 mRNA. Translation: AAK59628.2.
AY057481 mRNA. Translation: AAL09715.1.
AY120747 mRNA. Translation: AAM53305.1.
BT002754 mRNA. Translation: AAO22583.1.
M23108, M23105 Genomic DNA. Translation: AAA32819.1.
X77199 Genomic DNA. Translation: CAA54419.1.
PIRiS46302.
T48271.
RefSeqiNP_195870.1. NM_120328.4. [P22953-1]
UniGeneiAt.23663.

Genome annotation databases

EnsemblPlantsiAT5G02500.1; AT5G02500.1; AT5G02500. [P22953-1]
GeneIDi831020.
KEGGiath:AT5G02500.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74604 mRNA. Translation: CAA52684.1.
AL162971 Genomic DNA. Translation: CAB85987.1.
CP002688 Genomic DNA. Translation: AED90480.1.
AY035123 mRNA. Translation: AAK59628.2.
AY057481 mRNA. Translation: AAL09715.1.
AY120747 mRNA. Translation: AAM53305.1.
BT002754 mRNA. Translation: AAO22583.1.
M23108, M23105 Genomic DNA. Translation: AAA32819.1.
X77199 Genomic DNA. Translation: CAA54419.1.
PIRiS46302.
T48271.
RefSeqiNP_195870.1. NM_120328.4. [P22953-1]
UniGeneiAt.23663.

3D structure databases

ProteinModelPortaliP22953.
SMRiP22953. Positions 6-619.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16298. 28 interactions.
IntActiP22953. 9 interactions.
MINTiMINT-8061914.
STRINGi3702.AT5G02500.1.

Protein family/group databases

TCDBi1.A.33.1.1. the cation channel-forming heat shock protein-70 (hsp70) family.

PTM databases

iPTMnetiP22953.

2D gel databases

SWISS-2DPAGEP22953.
World-2DPAGE0003:P22953.

Proteomic databases

PaxDbiP22953.
PRIDEiP22953.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G02500.1; AT5G02500.1; AT5G02500. [P22953-1]
GeneIDi831020.
KEGGiath:AT5G02500.

Organism-specific databases

TAIRiAT5G02500.

Phylogenomic databases

eggNOGiKOG0101. Eukaryota.
COG0443. LUCA.
HOGENOMiHOG000228135.
InParanoidiP22953.
KOiK03283.
OMAiPMIYVEY.
OrthoDBiEOG093604KP.
PhylomeDBiP22953.

Enzyme and pathway databases

BioCyciARA:GQT-225-MONOMER.
ARA:GQT-44-MONOMER.
ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371568. Attenuation phase.
R-ATH-3371571. HSF1-dependent transactivation.
R-ATH-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiP22953.

Gene expression databases

ExpressionAtlasiP22953. baseline and differential.
GenevisibleiP22953. AT.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMD37E_ARATH
AccessioniPrimary (citable) accession number: P22953
Secondary accession number(s): Q93VU6, Q9LZ52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing HSC70-1 show disabled immune responses, enhanced tolerance to heat shock and altered plant growth and development.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.