P22949MCRB_METTEMethyl-coenzyme M reductase subunit beta2.8.4.1Coenzyme-B sulfoethylthiotransferase betaMethanosarcina thermophilaArchaeaEuryarchaeotaStenosarchaea groupMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcinaPurification and properties of methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila.PROTEIN SEQUENCEFUNCTIONCATALYTIC ACTIVITYBIOPHYSICOCHEMICAL PROPERTIESCOFACTORSUBUNITSUBCELLULAR LOCATIONComponent of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methanecoenzyme F430Binds 1 coenzyme F430 non-covalently per MCR heterotrimeric complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced, probably to the Ni(I) oxidation state.Optimum pH is 7.0.Optimum temperature is 60 degrees Celsius.One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.MCR from M.thermophila is a heterotrimer composed of an alpha, a beta, and a gamma subunit.CytoplasmBelongs to the methyl-coenzyme M reductase beta subunit family.CytoplasmDirect protein sequencingMethanogenesisTransferaseSDTVDIYDAGKILERVII
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