Reviewed, UniProtKB/Swiss-Prot P22949 (MCRB_METTE)

Names and origin

Protein names	Recommended name:     Methyl-coenzyme M reductase subunit beta     EC=2.8.4.1 Alternative name(s):     Coenzyme-B sulfoethylthiotransferase beta
Organism	Methanosarcina thermophila
Taxonomic identifier	2210 [NCBI]
Taxonomic lineage	Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	18 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide.
Catalytic activity	2-(methylthio)ethanesulfonate (methyl-CoM) + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid Potential.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Heterotrimer composed of an alpha, a beta, and a gamma subunit.
Miscellaneous	Reduced ferredoxin could reductively reactivate the enzyme.
Biophysicochemical properties	Temperature dependence: Optimum temperature is 60 degrees Celsius.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›18	›18	Methyl-coenzyme M reductase subunit beta		PRO_0000147470
Experimental info
	Non-terminal residue	18	1

Sequences

	Sequence		Length	Mass (Da)
	P22949-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 3783D55E2C6E369D	FASTA	18	2,020
	10 SDTVDIYDAG KILERVII

References

[1]	"Purification and properties of methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila." Jablonski P.E., Ferry J.G. J. Bacteriol. 173:2481-2487(1991) [PubMed: 2013570] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: DSM 1825 / TM-1.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.8.4.1. 8918.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

MCRB_METTE

Accession

Primary (citable) accession number: P22949

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	September 22, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)