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Protein

Nitrite reductase [NAD(P)H]

Gene

niiA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Ammonia + 3 NAD(P)+ + 2 H2O = nitrite + 3 NAD(P)H.

Cofactori

Protein has several cofactor binding sites:
  • sirohemeNote: Binds 1 siroheme per subunit.
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity
  • FAD
  • [2Fe-2S] clusterPROSITE-ProRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit.PROSITE-ProRule annotation

Pathwayi: nitrate reduction (assimilation)

This protein is involved in the pathway nitrate reduction (assimilation), which is part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the pathway nitrate reduction (assimilation) and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi720 – 7201Iron-sulfur (4Fe-4S)By similarity
Metal bindingi726 – 7261Iron-sulfur (4Fe-4S)By similarity
Metal bindingi760 – 7601Iron-sulfur (4Fe-4S)By similarity
Metal bindingi764 – 7641Iron (siroheme axial ligand)By similarity
Metal bindingi764 – 7641Iron-sulfur (4Fe-4S)By similarity
Metal bindingi976 – 9761Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi978 – 9781Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation
Metal bindingi1001 – 10011Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation
Metal bindingi1004 – 10041Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi44 – 7936FADSequence analysisAdd
BLAST
Nucleotide bindingi146 – 17631NAD or NADPSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • nitrate assimilation Source: UniProtKB-UniPathway
  • nitrogen compound metabolic process Source: ASPGD
  • sulfate reduction Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

2Fe-2S, 4Fe-4S, FAD, Flavoprotein, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00653.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrite reductase [NAD(P)H] (EC:1.7.1.4)
Gene namesi
Name:niiA
ORF Names:AN1007
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VIII
  • UP000005890 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AN1007.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11041104Nitrite reductase [NAD(P)H]PRO_0000199958Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP22944.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini932 – 1040109RieskePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000196164.
InParanoidiP22944.
KOiK17877.
OMAiYREQARY.
OrthoDBiEOG092C0BFS.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
IPR023753. FAD/NAD-binding_dom.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR012748. Nitri_red_NirD.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF04324. Fer2_BFD. 1 hit.
PF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF13806. Rieske_2. 1 hit.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF55124. SSF55124. 1 hit.
TIGRFAMsiTIGR02378. nirD_assim_sml. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
PS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22944-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLLDGPRNG ETVTASAHNG IPIIDGVDPS TLRGDIDQDP NRRQKIVVVG
60 70 80 90 100
LGMVAVAFIE KLVKLDSERR KYDIVVIGEE PHIAYNRVGL SSYFEHRKIE
110 120 130 140 150
DLYLNPKEWY GSFKDRSFDY YLNTRVTDVF PQHKTVKTST GDIVSYDILV
160 170 180 190 200
LATGSDAVLP TSTPGHDAKG IFVYRTISDL ERLMEFAANH KGQTGVTVGG
210 220 230 240 250
GLLGLEAAKA MTDLEDFGSV KLIDRNKWVL ARQLDGDAGS LVTKKIRDLG
260 270 280 290 300
LEVLHEKRVA KIHTDDDNNV TGILFEDGQE LDCCCICFAI GIRPRDELGG
310 320 330 340 350
STGIQCAKRG GFVIDESLRT SVNDIYAIGE CASWENQTFG IIAPGIEMAD
360 370 380 390 400
VLSFNLTNPD KEPKRFNRPD LSTKLKLLGV DVASFGDFFA DRDGPKFLPG
410 420 430 440 450
QRPSAESIGA ADPNREEEPQ VKALTYRDPF GGVYKKYLFT MDGKYLLGGM
460 470 480 490 500
MIGDTKDYVK LNQMVKSQKP LEVPPSEFIL GAQSGGEENA DDLDDSTQIC
510 520 530 540 550
SCHNVTKGDV VESVKSGTCK TIADVKSCTK AGTGCGGCMP LVQSIFNKTM
560 570 580 590 600
LDMGQEVSNN LCVHIPYSRA DLYNVIAIRQ LRTFDDVMKS AGKCPDSLGC
610 620 630 640 650
EICKPAIASI LSSLFNPHLM DKEYHELQET NDRFLANIQR NGTFSVVPRV
660 670 680 690 700
PGGEITADKL IAIGQVAKKY NLYCKITGGQ RIDMFGARKQ DLLDIWTELV
710 720 730 740 750
DAGMESGHAY AKSLRTVKSC VGTTWCRFGV GDSVGMAIRL EQRYKSIRAP
760 770 780 790 800
HKFKGAVSGC VRECAEAQNK DFGLIATEKG FNIFVGGNGG AKPRHSELLA
810 820 830 840 850
KDVPPEEVIP ILDRYVIFYI RTADKLQRTA RWLESLPGGI EYLKDVVLND
860 870 880 890 900
KLGIAAEMER QMQELVDSYF CEWTETVRNP KRRKYFQQFA NTDETVENVE
910 920 930 940 950
IVKEREQVRP TYWPKDGANE DFKGHQWSSL SWQPVIKADY FSDGPPAISS
960 970 980 990 1000
ANIKRGDTQL AIFKVKGKYY ATQQMCPHKR TFVLSDGLIG DDDNGKYWVS
1010 1020 1030 1040 1050
CPYHKRNFEL NGEQAGRCQN DEAMNIATFP VEEREDGWIY MKLPPVEELD
1060 1070 1080 1090 1100
SVLGTEKWKV KKGEAVDPFE AYDKKYSGMK GKRAGAKGIE GSKPTRSPSN

TIDW
Length:1,104
Mass (Da):122,646
Last modified:May 26, 2009 - v2
Checksum:i03CEA99309D7F691
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti709 – 7091A → R in AAA33315 (PubMed:2205530).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58289 Genomic DNA. Translation: AAA33315.1.
AACD01000015 Genomic DNA. Translation: EAA65575.1.
BN001308 Genomic DNA. Translation: CBF88349.1.
PIRiJH0181.
RefSeqiXP_658611.1. XM_653519.1.

Genome annotation databases

EnsemblFungiiCADANIAT00001642; CADANIAP00001642; CADANIAG00001642.
EAA65575; EAA65575; AN1007.2.
GeneIDi2876784.
KEGGiani:AN1007.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58289 Genomic DNA. Translation: AAA33315.1.
AACD01000015 Genomic DNA. Translation: EAA65575.1.
BN001308 Genomic DNA. Translation: CBF88349.1.
PIRiJH0181.
RefSeqiXP_658611.1. XM_653519.1.

3D structure databases

ProteinModelPortaliP22944.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00001642; CADANIAP00001642; CADANIAG00001642.
EAA65575; EAA65575; AN1007.2.
GeneIDi2876784.
KEGGiani:AN1007.2.

Organism-specific databases

EuPathDBiFungiDB:AN1007.

Phylogenomic databases

HOGENOMiHOG000196164.
InParanoidiP22944.
KOiK17877.
OMAiYREQARY.
OrthoDBiEOG092C0BFS.

Enzyme and pathway databases

UniPathwayiUPA00653.

Family and domain databases

Gene3Di2.102.10.10. 1 hit.
3.50.50.60. 2 hits.
InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
IPR023753. FAD/NAD-binding_dom.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR012748. Nitri_red_NirD.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF04324. Fer2_BFD. 1 hit.
PF01077. NIR_SIR. 1 hit.
PF03460. NIR_SIR_ferr. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF13806. Rieske_2. 1 hit.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF51905. SSF51905. 2 hits.
SSF55124. SSF55124. 1 hit.
TIGRFAMsiTIGR02378. nirD_assim_sml. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
PS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIR_EMENI
AccessioniPrimary (citable) accession number: P22944
Secondary accession number(s): C8VU59, Q5BEM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: May 26, 2009
Last modified: September 7, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.