12 kDa heat shock protein - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P22943 (HSP12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
12 kDa heat shock protein

Alternative name(s):
Glucose and lipid-regulated protein

Gene names

Name:	HSP12
Synonyms:	GLP1, HOR5
Ordered Locus Names:	YFL014W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	109 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in a switch from carbohydrate utilizing metabolism to fatty acid utilizing metabolism.
Induction	Strong, by heat shock, by entry in the stationary growth phase, and by cAMP, probably via the activity of a cAMP-dependent protein kinase. By glucose starvation and by fatty acids.
Miscellaneous	Present with 4490 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	To S.pombe hsp9 and C.albicans WH11.

Ontologies

Keywords
Biological process	Stress response
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from direct assay Ref.7. Source: SGD cellular response to heat Inferred from mutant phenotype. Source: SGD cellular response to osmotic stress Inferred from mutant phenotype. Source: SGD cellular response to oxidative stress Inferred from mutant phenotype. Source: SGD plasma membrane organization Inferred from mutant phenotype. Source: SGD
Cellular component	cytosol Inferred from direct assay. Source: SGD endosome Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD plasma membrane Inferred from direct assay. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	lipid binding Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 109

109

12 kDa heat shock protein

PRO_0000084081

Amino acid modifications

Modified residue

Phosphoserine; by ATM or ATR Ref.10 Ref.11 Ref.12

Modified residue

Phosphoserine Ref.10 Ref.11 Ref.12

Modified residue

Phosphoserine Ref.11 Ref.12

Modified residue

Phosphoserine Ref.11 Ref.12

Modified residue

Phosphoserine Ref.11 Ref.12

Modified residue

Phosphoserine Ref.12

Natural variations

Natural variant

P → T in strain: Ar5-H12.

Secondary structure

109

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P22943 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 7C73862689759B4A
Show »

FASTA

109

11,693

        10         20         30         40         50         60 
MSDAGRKGFG EKASEALKPD SQKSYAEQGK EYITDKADKV AGKVQPEDNK GVFQGVHDSA 

        70         80         90        100 
EKGKDNAEGQ GESLADQARD YMGAAKSKLN DAVEYVSGRV HGEEDPTKK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing and chromosomal assignment of a gene from Saccharomyces cerevisiae which is negatively regulated by glucose and positively by lipids." Stone R.L., Matarese V., Magee B.B., Magee P.T., Bernlohr D.A. Gene 96:171-176(1990) [PubMed: 2129531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 204508 / S288c.
[2]	"HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function." Praekelt U.M., Meacock P.A. Mol. Gen. Genet. 223:97-106(1990) [PubMed: 2175390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 204508 / S288c.
[3]	Barrell B.G., Churcher C., Rajandream M.A. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 204511 / S288c / AB972.
[4]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Transcriptional regulation of bottom-fermenting yeast specific HSP12 gene." Sone H., Tomizuka K., Suda H., Iwamatsu A., Kondo K., Inouye M., Tanaka J. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: KBY001.
[7]	"HSP12 is essential for biofilm formation by a Sardinian wine strain of Saccharomyces cerevisiae." Zara S., Farris G.A., Budroni M., Bakalinsky A.T. Yeast 19:269-276(2002) [PubMed: 11816034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Ar5-H12 / Arvisionadu wine isolate.
[8]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21 AND SER-24, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-24; SER-59; SER-73 AND SER-87, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-24; SER-59; SER-73; SER-87 AND SER-97, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M60827 Genomic DNA. Translation: AAA34647.1.
X55785 mRNA. Translation: CAA39306.1.
Z46255 Genomic DNA. Translation: CAA86349.1.
D50617 Genomic DNA. Translation: BAA09224.1.
D89864 Genomic DNA. Translation: BAA14033.1.
AY046957 Genomic DNA. Translation: AAL06077.1.
AY558464 Genomic DNA. Translation: AAS56790.1.
BK006940 Genomic DNA. Translation: DAA12425.1.

PIR

HHBY12. S11179.

RefSeq

NP_116640.1. NM_001179952.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L9Q	NMR	-	A	1-109	[»]
2LJL	NMR	-	A	1-109	[»]

ProteinModelPortal

P22943.

SMR

P22943. Positions 1-109.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6408N.

IntAct

P22943. 24 interactions.

MINT

MINT-700216.

STRING

P22943.

Proteomic databases

PeptideAtlas

P22943.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YFL014W; YFL014W; YFL014W.

GeneID

850532.

KEGG

sce:YFL014W.

NMPDR

fig|4932.3.peg.2269.

Organism-specific databases

CYGD

YFL014w.

SGD

S000001880. HSP12.

Phylogenomic databases

eggNOG

fuNOG10919.

GeneTree

EFGT00050000004925.

HOGENOM

HBG736631.

OMA

NDAVEYV.

OrthoDB

EOG43BQZP.

Gene expression databases

ArrayExpress

P22943.

Genevestigator

P22943.

GermOnline

YFL014W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR016360. Heat_shock_protein_9/12_fun.
IPR007250. HSP9_HSP12.
[Graphical view]

Pfam

PF04119. HSP9_HSP12. 1 hit.
[Graphical view]

PIRSF

PIRSF002590. HSP9/HSP12_fun. 1 hit.

ProtoNet

Search...

Other

NextBio

966280.

Entry information

Entry name

HSP12_YEAST

Accession

Primary (citable) accession number: P22943
Secondary accession number(s): D6VTL5, Q8X145

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	December 14, 2011
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Natural variations

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents