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Reviewed, UniProtKB/Swiss-Prot P22939 (ISPA_ECOLI)

Last modified June 16, 2009. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Geranyltranstransferase
    EC=2.5.1.10
Alternative name(s):
    Farnesyl-diphosphate synthase
      Short name=FPP synthase
Gene names
Name: ispA
Ordered Locus Names: b0421, JW0411
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the FPP/GGPP synthetase family.

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Ontologies

Keywords
   Biological processIsoprene biosynthesis
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processisoprenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiongeranyltranstransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 299299Geranyltranstransferase
PRO_0000123982

Secondary structure

1 ....................................... 299
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P22939-1 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 15BADD5E135060CA

FASTA29932,160
        10         20         30         40         50         60 
MDFPQQLEAC VKQANQALSR FIAPLPFQNT PVVETMQYGA LLGGKRLRPF LVYATGHMFG 

        70         80         90        100        110        120 
VSTNTLDAPA AAVECIHAYS LIHDDLPAMD DDDLRRGLPT CHVKFGEANA ILAGDALQTL 

       130        140        150        160        170        180 
AFSILSDADM PEVSDRDRIS MISELASASG IAGMCGGQAL DLDAEGKHVP LDALERIHRH 

       190        200        210        220        230        240 
KTGALIRAAV RLGALSAGDK GRRALPVLDK YAESIGLAFQ VQDDILDVVG DTATLGKRQG 

       250        260        270        280        290 
ADQQLGKSTY PALLGLEQAR KKARDLIDDA RQSLKQLAEQ SLDTSALEAL ADYIIQRNK

« Hide

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References

« Hide 'large scale' references
[1]"Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli."
Fujisaki S., Hara H., Nishimura Y., Horiuchi K., Nishino T.
J. Biochem. 108:995-1000(1990) [PubMed: 2089044] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

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Cross-references

Sequence databases

D00694 Genomic DNA. Translation: BAA00599.1.
U82664 Genomic DNA. Translation: AAB40177.1.
U00096 Genomic DNA. Translation: AAC73524.1.
AP009048 Genomic DNA. Translation: BAE76201.1.
PIRJQ0665.
RefSeqAP_001071.1.
NP_414955.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1RQIX-ray2.42A/B1-299[»]
1RQJX-ray1.95A/B1-299[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:10044N.

2-D gel databases

SWISS-2DPAGEP22939.

Genome annotation databases

GeneID945064.
GenomeReviewsGene locus JW0411 in contig AP009048_GR.
Gene locus b0421 in contig U00096_GR.
KEGGecj:JW0411.
eco:b0421.

Organism-specific databases

EchoBASEEB0503.
EcoGeneEG10508. ispA.
CMRSearch...

Phylogenomic databases

HOGENOMP22939.
OMAP22939. QVQDDII.

Enzyme and pathway databases

BioCycEcoCyc:FPPSYN-MON.
MetaCyc:FPPSYN-MON.
BRENDA2.5.1.1. 246.
2.5.1.10. 246.

Family and domain databases

InterProIPR000092. Polyprenyl_synt.
IPR017446. Polyprenyl_synth-rel.
IPR008949. Terpenoid_synth.
[Graphical view]
Gene3DG3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHERPTHR12001. Polyprenyl_synt. 1 hit.
PfamPF00348. polyprenyl_synt. 1 hit.
[Graphical view]
PROSITEPS00723. POLYPRENYL_SYNTHET_1. 1 hit.
PS00444. POLYPRENYL_SYNTHET_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameISPA_ECOLI
AccessionPrimary (citable) accession number: P22939
Secondary accession number(s): Q2MC05
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents