Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Farnesyl diphosphate synthase

Gene

ispA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate.

Cofactori

Mg2+1 PublicationNote: Binds 3 Mg2+ ions per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45Isopentenyl diphosphate1 Publication1
Binding sitei48Isopentenyl diphosphate1 Publication1
Binding sitei77Isopentenyl diphosphate1 Publication1
Metal bindingi84Magnesium 11 Publication1
Metal bindingi84Magnesium 21 Publication1
Metal bindingi90Magnesium 11 Publication1
Metal bindingi90Magnesium 21 Publication1
Binding sitei95Geranyl diphosphate1
Binding sitei96Isopentenyl diphosphate1 Publication1
Binding sitei181Geranyl diphosphate1
Binding sitei182Geranyl diphosphate1
Binding sitei220Geranyl diphosphate1
Metal bindingi223Magnesium 31 Publication1
Binding sitei237Geranyl diphosphate1

GO - Molecular functioni

  • dimethylallyltranstransferase activity Source: EcoCyc
  • geranyltranstransferase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • farnesyl diphosphate biosynthetic process Source: EcoCyc
  • geranyl diphosphate biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionTransferase
Biological processIsoprene biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:FPPSYN-MONOMER
MetaCyc:FPPSYN-MONOMER
SABIO-RKiP22939

Names & Taxonomyi

Protein namesi
Recommended name:
Farnesyl diphosphate synthase (EC:2.5.1.10)
Short name:
FPP synthase
Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Geranyltranstransferase
Gene namesi
Name:ispA
Ordered Locus Names:b0421, JW0411
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10508 ispA

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075078
DrugBankiDB02270 Dimethylallyl S-Thiolodiphosphate
DB04160 Diphosphate
DB02508 Isopentyl Pyrophosphate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001239821 – 299Farnesyl diphosphate synthaseAdd BLAST299

Proteomic databases

PaxDbiP22939
PRIDEiP22939

2D gel databases

SWISS-2DPAGEiP22939

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4259837, 475 interactors
DIPiDIP-10044N
IntActiP22939, 16 interactors
STRINGi316385.ECDH10B_0377

Structurei

Secondary structure

1299
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 22Combined sources20
Beta strandi25 – 27Combined sources3
Helixi31 – 41Combined sources11
Helixi47 – 58Combined sources12
Helixi63 – 84Combined sources22
Turni87 – 90Combined sources4
Helixi101 – 105Combined sources5
Helixi107 – 127Combined sources21
Helixi135 – 149Combined sources15
Helixi154 – 163Combined sources10
Turni164 – 167Combined sources4
Helixi171 – 181Combined sources11
Helixi183 – 195Combined sources13
Turni196 – 198Combined sources3
Helixi199 – 229Combined sources31
Helixi232 – 235Combined sources4
Helixi241 – 245Combined sources5
Helixi250 – 254Combined sources5
Helixi256 – 278Combined sources23
Turni279 – 281Combined sources3
Helixi285 – 296Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RQIX-ray2.42A/B1-299[»]
1RQJX-ray1.95A/B1-299[»]
ProteinModelPortaliP22939
SMRiP22939
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22939

Family & Domainsi

Sequence similaritiesi

Belongs to the FPP/GGPP synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105CTB Bacteria
COG0142 LUCA
HOGENOMiHOG000009101
InParanoidiP22939
KOiK00795
OMAiCEGQALD
PhylomeDBiP22939

Family and domain databases

Gene3Di1.10.600.10, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR000092 Polyprenyl_synt
IPR033749 Polyprenyl_synt_CS
PfamiView protein in Pfam
PF00348 polyprenyl_synt, 1 hit
SUPFAMiSSF48576 SSF48576, 1 hit
PROSITEiView protein in PROSITE
PS00723 POLYPRENYL_SYNTHASE_1, 1 hit
PS00444 POLYPRENYL_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P22939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFPQQLEAC VKQANQALSR FIAPLPFQNT PVVETMQYGA LLGGKRLRPF
60 70 80 90 100
LVYATGHMFG VSTNTLDAPA AAVECIHAYS LIHDDLPAMD DDDLRRGLPT
110 120 130 140 150
CHVKFGEANA ILAGDALQTL AFSILSDADM PEVSDRDRIS MISELASASG
160 170 180 190 200
IAGMCGGQAL DLDAEGKHVP LDALERIHRH KTGALIRAAV RLGALSAGDK
210 220 230 240 250
GRRALPVLDK YAESIGLAFQ VQDDILDVVG DTATLGKRQG ADQQLGKSTY
260 270 280 290
PALLGLEQAR KKARDLIDDA RQSLKQLAEQ SLDTSALEAL ADYIIQRNK
Length:299
Mass (Da):32,160
Last modified:August 1, 1991 - v1
Checksum:i15BADD5E135060CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00694 Genomic DNA Translation: BAA00599.1
U82664 Genomic DNA Translation: AAB40177.1
U00096 Genomic DNA Translation: AAC73524.1
AP009048 Genomic DNA Translation: BAE76201.1
PIRiJQ0665
RefSeqiNP_414955.1, NC_000913.3
WP_000347217.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73524; AAC73524; b0421
BAE76201; BAE76201; BAE76201
GeneIDi945064
KEGGiecj:JW0411
eco:b0421
PATRICifig|1411691.4.peg.1856

Similar proteinsi

Entry informationi

Entry nameiISPA_ECOLI
AccessioniPrimary (citable) accession number: P22939
Secondary accession number(s): Q2MC05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: March 28, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health