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P22936 (APN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-(apurinic or apyrimidinic site) lyase 1

EC=4.2.99.18
Alternative name(s):
Apurinic-apyrimidinic endonuclease 1
Short name=AP endonuclease 1
Gene names
Name:APN1
Ordered Locus Names:YKL114C
ORF Names:YKL513
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA. APN1 accounts for > 97% of both apurinic/ apyrimidinic (AP) lyase and DNA 3'-repair diesterase activities. HAMAP-Rule MF_00152

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00152

Cofactor

Binds 3 zinc ions.

Subunit structure

Monomer.

Subcellular location

Nucleus HAMAP-Rule MF_00152.

Miscellaneous

Present with 7250 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the AP endonuclease 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 367366DNA-(apurinic or apyrimidinic site) lyase 1 HAMAP-Rule MF_00152
PRO_0000190898

Sites

Metal binding831Zinc 1 By similarity
Metal binding1231Zinc 1 By similarity
Metal binding1581Zinc 1 By similarity
Metal binding1581Zinc 2 By similarity
Metal binding1921Zinc 2 By similarity
Metal binding1951Zinc 3 By similarity
Metal binding2291Zinc 2 By similarity
Metal binding2421Zinc 3 By similarity
Metal binding2441Zinc 3 By similarity
Metal binding2741Zinc 2 By similarity

Amino acid modifications

Modified residue3561Phosphoserine Ref.8

Experimental info

Sequence conflict2391A → S in AAA34429. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P22936 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 76C14935B760A6D1

FASTA36741,439
        10         20         30         40         50         60 
MPSTPSFVRS AVSKYKFGAH MSGAGGISNS VTNAFNTGCN SFAMFLKSPR KWVSPQYTQE 

        70         80         90        100        110        120 
EIDKFKKNCA TYNYNPLTDV LPHGQYFINL ANPDREKAEK SYESFMDDLN RCEQLGIGLY 

       130        140        150        160        170        180 
NLHPGSTLKG DHQLQLKQLA SYLNKAIKET KFVKIVLENM AGTGNLVGSS LVDLKEVIGM 

       190        200        210        220        230        240 
IEDKSRIGVC IDTCHTFAAG YDISTTETFN NFWKEFNDVI GFKYLSAVHL NDSKAPLGAN 

       250        260        270        280        290        300 
RDLHERLGQG YLGIDVFRMI AHSEYLQGIP IVLETPYEND EGYGNEIKLM EWLESKSESE 

       310        320        330        340        350        360 
LLEDKEYKEK NDTLQKLGAK SRKEQLDKFE VKQKKRAGGT KRKKATAEPS DNDILSQMTK 


KRKTKKE 

« Hide

References

« Hide 'large scale' references
[1]"Yeast structural gene (APN1) for the major apurinic endonuclease: homology to Escherichia coli endonuclease IV."
Popoff S.C., Spira A.I., Johnson A.W., Demple B.
Proc. Natl. Acad. Sci. U.S.A. 87:4193-4197(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases."
Jacquier A., Legrain P., Dujon B.
Yeast 8:121-132(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Yeast DNA diesterase for 3'-fragments of deoxyribose: purification and physical properties of a repair enzyme for oxidative DNA damage."
Johnson A.W., Demple B.
J. Biol. Chem. 263:18009-18016(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 2-26.
[6]"Metalloenzymes in DNA repair. Escherichia coli endonuclease IV and Saccharomyces cerevisiae Apn1."
Levin J.D., Shapiro R., Demple B.
J. Biol. Chem. 266:22893-22898(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: METAL-BINDING STUDIES.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-356, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M33667 Genomic DNA. Translation: AAA34429.1.
S93804 Genomic DNA. Translation: AAB22003.1.
Z28114 Genomic DNA. Translation: CAA81954.1.
BK006944 Genomic DNA. Translation: DAA09044.1.
PIRS29871.
RefSeqNP_012808.1. NM_001179680.1.

3D structure databases

ProteinModelPortalP22936.
SMRP22936. Positions 18-296.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34020. 82 interactions.
DIPDIP-4106N.
IntActP22936. 2 interactions.
MINTMINT-537191.
STRING4932.YKL114C.

Proteomic databases

PaxDbP22936.
PeptideAtlasP22936.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL114C; YKL114C; YKL114C.
GeneID853746.
KEGGsce:YKL114C.

Organism-specific databases

SGDS000001597. APN1.

Phylogenomic databases

eggNOGCOG0648.
GeneTreeENSGT00390000013698.
HOGENOMHOG000224893.
KOK10771.
OMAPREPMER.
OrthoDBEOG71VT48.

Enzyme and pathway databases

BioCycYEAST:G3O-31899-MONOMER.
BRENDA4.2.99.18. 984.

Gene expression databases

GenevestigatorP22936.

Family and domain databases

Gene3D3.20.20.150. 1 hit.
HAMAPMF_00152. Nfo.
InterProIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERPTHR21445. PTHR21445. 1 hit.
PfamPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMSSF51658. SSF51658. 1 hit.
TIGRFAMsTIGR00587. nfo. 1 hit.
PROSITEPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974804.
PROP22936.

Entry information

Entry nameAPN1_YEAST
AccessionPrimary (citable) accession number: P22936
Secondary accession number(s): D6VXH4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 129 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families