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Protein

Chalcone synthase B

Gene

CHSB

Organism
Petunia hybrida (Petunia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.PROSITE-ProRule annotation

Pathway: flavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei167 – 1671PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Flavonoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Chalcone synthase B (EC:2.3.1.74)
Alternative name(s):
Naringenin-chalcone synthase B
Gene namesi
Name:CHSB
OrganismiPetunia hybrida (Petunia)
Taxonomic identifieri4102 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Chalcone synthase BPRO_0000216030Add
BLAST

Expressioni

Tissue specificityi

Expressed at low level in seedlings after illumination with UV light. No expression in flowers or tissue culture.

Structurei

3D structure databases

ProteinModelPortaliP22924.
SMRiP22924. Positions 13-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chalcone/stilbene synthases family.Curated

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22924-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVENGQLQG WWAQRAEGPA KILAIGTATP FHWVDQNSYP DYYFRVTNSQ
60 70 80 90 100
HLVDLKEKFR RICSKTMIKK RHMFLTEELL RKNPTLCSHN EPSLDIRQDI
110 120 130 140 150
LVSEIPKLGK EAALKAIGEW GQPKSTITHL VFCTRSGVDM PGADCQLVKL
160 170 180 190 200
LGLSPSVQRL MMYQQGCFAG GTMLRLAKDL AENNKGARVL VVCAESSAIG
210 220 230 240 250
FRGPSEANVD NLIAQALFGD GAAALIIGSD PKPGLERPVF EIFSAAQTFV
260 270 280 290 300
PNGDCHLALH LREMGLTFHC TKDVPPTIAK NVESCLIKAF EPLGISDWNS
310 320 330 340 350
LFWILHPGGN AIVDQVESTL GLGPEKLRAT RNILSEYGNL SSACCLFILD
360 370 380 390
EIRKKSAREG MRTSGDGLDL GVLLSFGPGL TIETVVLRSV PI
Length:392
Mass (Da):42,957
Last modified:August 1, 1991 - v1
Checksum:i52B2F22515287274
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14592 Genomic DNA. Translation: CAA32732.1.
PIRiJS0311. SYPJCB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14592 Genomic DNA. Translation: CAA32732.1.
PIRiJS0311. SYPJCB.

3D structure databases

ProteinModelPortaliP22924.
SMRiP22924. Positions 13-392.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00154.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000451. PKS_III. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
PROSITEiPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and molecular characterization of the chalcone synthase multigene family of Petunia hybrida."
    Koes R.E., Spelt C.E., van den Elzen P.J.M., Mol J.N.M.
    Gene 81:245-257(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Violet 30.
    Tissue: Leaf.

Entry informationi

Entry nameiCHSB_PETHY
AccessioniPrimary (citable) accession number: P22924
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 1, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.