Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-2A adrenergic receptor

Gene

Adra2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei113 – 1131Implicated in ligand bindingBy similarity
Sitei200 – 2001Implicated in catechol agonist bindingBy similarity
Sitei204 – 2041Implicated in catechol agonist bindingBy similarity

GO - Molecular functioni

  • alpha2-adrenergic receptor activity Source: RGD
  • epinephrine binding Source: Ensembl
  • norepinephrine binding Source: Ensembl

GO - Biological processi

  • activation of MAPK activity by adrenergic receptor signaling pathway Source: Ensembl
  • activation of protein kinase B activity Source: Ensembl
  • acute inflammatory response Source: RGD
  • adenylate cyclase-inhibiting adrenergic receptor signaling pathway Source: GO_Central
  • cellular response to hormone stimulus Source: Ensembl
  • DNA replication Source: RGD
  • epidermal growth factor-activated receptor transactivation by G-protein coupled receptor signaling pathway Source: Ensembl
  • fear response Source: Ensembl
  • female pregnancy Source: RGD
  • glucose homeostasis Source: BHF-UCL
  • G-protein coupled receptor signaling pathway Source: BHF-UCL
  • negative regulation of adenylate cyclase activity Source: GO_Central
  • negative regulation of calcium ion-dependent exocytosis Source: BHF-UCL
  • negative regulation of insulin secretion involved in cellular response to glucose stimulus Source: BHF-UCL
  • negative regulation of lipid catabolic process Source: Ensembl
  • negative regulation of norepinephrine secretion Source: GO_Central
  • negative regulation of uterine smooth muscle contraction Source: RGD
  • phospholipase C-activating adrenergic receptor signaling pathway Source: Ensembl
  • platelet activation Source: InterPro
  • positive regulation of cell migration Source: Ensembl
  • positive regulation of cytokine production Source: Ensembl
  • positive regulation of membrane protein ectodomain proteolysis Source: Ensembl
  • positive regulation of vasodilation Source: RGD
  • positive regulation of wound healing Source: Ensembl
  • regulation of vasoconstriction Source: InterPro
  • signal transduction Source: RGD
  • thermoception Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_284101. Adrenaline signalling through Alpha-2 adrenergic receptor.
REACT_294981. Adrenoceptors.
REACT_339234. G alpha (i) signalling events.
REACT_342580. G alpha (z) signalling events.
REACT_351378. Adrenaline,noradrenaline inhibits insulin secretion.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2A adrenergic receptor
Alternative name(s):
Alpha-2A adrenoreceptor
Short name:
Alpha-2A adrenoceptor
Short name:
Alpha-2AAR
Alpha-2D adrenergic receptor
CA2-47
Gene namesi
Name:Adra2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2056. Adra2a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333ExtracellularBy similarityAdd
BLAST
Transmembranei34 – 5926Helical; Name=1By similarityAdd
BLAST
Topological domaini60 – 7011CytoplasmicBy similarityAdd
BLAST
Transmembranei71 – 9626Helical; Name=2By similarityAdd
BLAST
Topological domaini97 – 10610ExtracellularBy similarity
Transmembranei107 – 12923Helical; Name=3By similarityAdd
BLAST
Topological domaini130 – 14920CytoplasmicBy similarityAdd
BLAST
Transmembranei150 – 17324Helical; Name=4By similarityAdd
BLAST
Topological domaini174 – 19219ExtracellularBy similarityAdd
BLAST
Transmembranei193 – 21725Helical; Name=5By similarityAdd
BLAST
Topological domaini218 – 374157CytoplasmicBy similarityAdd
BLAST
Transmembranei375 – 39925Helical; Name=6By similarityAdd
BLAST
Topological domaini400 – 40910ExtracellularBy similarity
Transmembranei410 – 43021Helical; Name=7By similarityAdd
BLAST
Topological domaini431 – 45020CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: RGD
  • receptor complex Source: Ensembl
  • synapse Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450Alpha-2A adrenergic receptorPRO_0000069083Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi10 – 101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi14 – 141N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi106 ↔ 188PROSITE-ProRule annotation
Lipidationi442 – 4421S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

PTM databases

PhosphoSiteiP22909.

Expressioni

Gene expression databases

ExpressionAtlasiP22909. baseline and differential.
GenevestigatoriP22909.

Interactioni

Structurei

3D structure databases

ProteinModelPortaliP22909.
SMRiP22909. Positions 118-149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2A sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG106962.
InParanoidiP22909.
KOiK04138.
PhylomeDBiP22909.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR001946. ADRA2A_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF24. PTHR24248:SF24. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00558. ADRENRGCA2AR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSLQPDAGN SSWNGTEAPG GGTRATPYSL QVTLTLVCLA GLLMLFTVFG
60 70 80 90 100
NVLVIIAVFT SRALKAPQNL FLVSLASADI LVATLVIPFS LANEVMGYWY
110 120 130 140 150
FGKVWCEIYL ALDVLFCTSS IVHLCAISLD RYWSITQAIE YNLKRTPRRI
160 170 180 190 200
KAIIVTVWVI SAVISFPPLI SIEKKGAGGG QQPAEPSCKI NDQKWYVISS
210 220 230 240 250
SIGSFFAPCL IMILVYVRIY QIAKRRTRVP PSRRGPDACS APPGGADRRP
260 270 280 290 300
NGLGPERGAG TAGAEAEPLP TQLNGAPGEP APTRPRDGDA LDLEESSSSE
310 320 330 340 350
HAERPQGPGK PERGPRAKGK TKASQVKPGD SLPRRGPGAA GPGASGSGQG
360 370 380 390 400
EERAGGAKAS RWRGRQNREK RFTFVLAVVI GVFVVCWFPF FFTYTLIAVG
410 420 430 440 450
CPVPYQLFNF FFWFGYCNSS LNPVIYTIFN HDFRRAFKKI LCRGDRKRIV
Length:450
Mass (Da):48,940
Last modified:April 1, 1993 - v2
Checksum:iAFA078DCA3D612AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti147 – 1471P → R (PubMed:2177834).Curated
Sequence conflicti154 – 1585IVTVW → HCHCV (PubMed:2177834).Curated
Sequence conflicti252 – 2532GL → AV in AAA42034 (PubMed:1645350).Curated
Sequence conflicti264 – 2641A → G (PubMed:2177834).Curated
Sequence conflicti304 – 3052RP → PR in AAA42034 (PubMed:1645350).Curated
Sequence conflicti333 – 3331P → R in AAA42034 (PubMed:1645350).Curated
Sequence conflicti445 – 4451D → N (PubMed:2177834).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62372 Genomic DNA. Translation: AAA42034.1.
U79031 mRNA. Translation: AAC24959.1.
PIRiB40392.
JH0190.
RefSeqiNP_036871.3. NM_012739.3.
UniGeneiRn.170171.

Genome annotation databases

GeneIDi25083.
KEGGirno:25083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62372 Genomic DNA. Translation: AAA42034.1.
U79031 mRNA. Translation: AAC24959.1.
PIRiB40392.
JH0190.
RefSeqiNP_036871.3. NM_012739.3.
UniGeneiRn.170171.

3D structure databases

ProteinModelPortaliP22909.
SMRiP22909. Positions 118-149.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP22909.
ChEMBLiCHEMBL2093864.
GuidetoPHARMACOLOGYi25.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP22909.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25083.
KEGGirno:25083.

Organism-specific databases

CTDi150.
RGDi2056. Adra2a.

Phylogenomic databases

HOVERGENiHBG106962.
InParanoidiP22909.
KOiK04138.
PhylomeDBiP22909.

Enzyme and pathway databases

ReactomeiREACT_284101. Adrenaline signalling through Alpha-2 adrenergic receptor.
REACT_294981. Adrenoceptors.
REACT_339234. G alpha (i) signalling events.
REACT_342580. G alpha (z) signalling events.
REACT_351378. Adrenaline,noradrenaline inhibits insulin secretion.

Miscellaneous databases

NextBioi605338.
PROiP22909.

Gene expression databases

ExpressionAtlasiP22909. baseline and differential.
GenevestigatoriP22909.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR001946. ADRA2A_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF24. PTHR24248:SF24. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00558. ADRENRGCA2AR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of rat genomic clones encoding subtypes of the alpha 2-adrenergic receptor. Identification of a unique receptor subtype."
    Lanier S.M., Downing S., Duzic E., Homcy C.J.
    J. Biol. Chem. 266:10470-10478(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning, sequencing and expression of an alpha 2-adrenergic receptor complementary DNA from rat brain."
    Chalberg S.C., Duda T., Rhine J.A., Sharma R.K.
    Mol. Cell. Biochem. 97:161-172(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Structural, genetic and pharmacological identity of the rat alpha 2-adrenergic receptor subtype cA2-47 and its molecular characterization in rat adrenal, adrenocortical carcinoma and bovine retina."
    Wypijewski K., Duda T., Sharma R.K.
    Mol. Cell. Biochem. 144:181-190(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiADA2A_RAT
AccessioniPrimary (citable) accession number: P22909
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: April 1, 1993
Last modified: May 27, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.