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P22907

- HEM3_MOUSE

UniProt

P22907 - HEM3_MOUSE

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Protein

Porphobilinogen deaminase

Gene
Hmbs, Uros1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.UniRule annotation

Catalytic activityi

4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3.UniRule annotation

Cofactori

Binds 1 dipyrromethane group covalently.

Pathwayi

GO - Molecular functioni

  1. hydroxymethylbilane synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. peptidyl-pyrromethane cofactor linkage Source: InterPro
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

ReactomeiREACT_203298. Heme biosynthesis.
UniPathwayiUPA00251; UER00319.

Names & Taxonomyi

Protein namesi
Recommended name:
Porphobilinogen deaminase (EC:2.5.1.61)
Short name:
PBG-D
Alternative name(s):
Hydroxymethylbilane synthase
Short name:
HMBS
Pre-uroporphyrinogen synthase
Gene namesi
Name:Hmbs
Synonyms:Uros1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:96112. Hmbs.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 361360Porphobilinogen deaminaseUniRule annotationPRO_0000143035Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Modified residuei74 – 741N6-acetyllysine1 Publication
Modified residuei261 – 2611S-(dipyrrolylmethanemethyl)cysteine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP22907.
PRIDEiP22907.

Expressioni

Gene expression databases

ArrayExpressiP22907.
BgeeiP22907.
CleanExiMM_HMBS.
GenevestigatoriP22907.

Interactioni

Protein-protein interaction databases

BioGridi200321. 1 interaction.
IntActiP22907. 1 interaction.
MINTiMINT-4097350.

Structurei

3D structure databases

ProteinModelPortaliP22907.
SMRiP22907. Positions 18-356.

Family & Domainsi

Sequence similaritiesi

Belongs to the HMBS family.

Phylogenomic databases

eggNOGiCOG0181.
GeneTreeiENSGT00390000009083.
HOGENOMiHOG000228587.
HOVERGENiHBG000967.
InParanoidiQ3TIV0.
KOiK01749.
OMAiGIECRTD.
OrthoDBiEOG7W41C4.
TreeFamiTF105389.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
HAMAPiMF_00260. Porphobil_deam.
InterProiIPR000860. 4pyrrol_synth_OHMeBilane_synth.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERiPTHR11557. PTHR11557. 1 hit.
PfamiPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 2 hits.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P22907-1) [UniParc]FASTAAdd to Basket

Also known as: Non-erythropoietic

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSGNGGAATT AEENGSKMRV IRVGTRKSQL ARIQTDTVVA MLKALYPGIQ    50
FEIIAMSTTG DKILDTALSK IGEKSLFTKE LENALEKNEV DLVVHSLKDV 100
PTILPPGFTI GAICKRENPC DAVVFHPKFI GKTLETLPEK SAVGTSSLRR 150
VAQLQRKFPH LEFKSIRGNL NTRLRKLDEL QEFSAIVLAV AGLQRMGWQN 200
RVGQILHPEE CMYAVGQGAL AVEVRAKDQD ILDLVSVLHD PETLLRCIAE 250
RAFLRHLEGG CSVPVAVHTV MKDGQLYLTG GVWSLDGSDS MQETMQATIQ 300
VPVQQEDGPE DDPQLVGITA RNIPRGAQLA AENLGISLAS LLLNKGAKNI 350
LDVARQLNDV R 361
Length:361
Mass (Da):39,344
Last modified:July 27, 2011 - v2
Checksum:iF184C439F4F7B55D
GO
Isoform 2 (identifier: P22907-2) [UniParc]FASTAAdd to Basket

Also known as: Erythrocyte

The sequence of this isoform differs from the canonical sequence as follows:
     2-17: Missing.

Show »
Length:345
Mass (Da):37,912
Checksum:iD74599AABD83A6BA
GO

Sequence cautioni

The sequence AAA39890.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei2 – 1716Missing in isoform 2. VSP_002068Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361D → E in AAA39890. 1 Publication
Sequence conflicti36 – 361D → E in AAA39891. 1 Publication
Sequence conflicti64 – 641L → V in AAA39890. 1 Publication
Sequence conflicti64 – 641L → V in AAA39891. 1 Publication
Sequence conflicti117 – 1171E → Q in AAA39890. 1 Publication
Sequence conflicti117 – 1171E → Q in AAA39891. 1 Publication
Sequence conflicti160 – 1601H → N in AAA39890. 1 Publication
Sequence conflicti160 – 1601H → N in AAA39891. 1 Publication
Sequence conflicti271 – 2711M → I in AAA39890. 1 Publication
Sequence conflicti271 – 2711M → I in AAA39891. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M28666
, M28663, M28664, M28665 Genomic DNA. Translation: AAA39890.1. Sequence problems.
M28666
, M28663, M28664, M28665 Genomic DNA. Translation: AAA39891.1.
AK166734 mRNA. Translation: BAE38979.1.
AK167702 mRNA. Translation: BAE39746.1.
CCDSiCCDS23106.1. [P22907-1]
PIRiA36513. IBMSN.
RefSeqiNP_038579.2. NM_013551.2. [P22907-1]
UniGeneiMm.247676.

Genome annotation databases

EnsembliENSMUST00000077353; ENSMUSP00000076575; ENSMUSG00000032126. [P22907-1]
GeneIDi15288.
KEGGimmu:15288.
UCSCiuc009pcz.2. mouse. [P22907-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M28666
, M28663 , M28664 , M28665 Genomic DNA. Translation: AAA39890.1 . Sequence problems.
M28666
, M28663 , M28664 , M28665 Genomic DNA. Translation: AAA39891.1 .
AK166734 mRNA. Translation: BAE38979.1 .
AK167702 mRNA. Translation: BAE39746.1 .
CCDSi CCDS23106.1. [P22907-1 ]
PIRi A36513. IBMSN.
RefSeqi NP_038579.2. NM_013551.2. [P22907-1 ]
UniGenei Mm.247676.

3D structure databases

ProteinModelPortali P22907.
SMRi P22907. Positions 18-356.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200321. 1 interaction.
IntActi P22907. 1 interaction.
MINTi MINT-4097350.

Proteomic databases

PaxDbi P22907.
PRIDEi P22907.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000077353 ; ENSMUSP00000076575 ; ENSMUSG00000032126 . [P22907-1 ]
GeneIDi 15288.
KEGGi mmu:15288.
UCSCi uc009pcz.2. mouse. [P22907-1 ]

Organism-specific databases

CTDi 3145.
MGIi MGI:96112. Hmbs.

Phylogenomic databases

eggNOGi COG0181.
GeneTreei ENSGT00390000009083.
HOGENOMi HOG000228587.
HOVERGENi HBG000967.
InParanoidi Q3TIV0.
KOi K01749.
OMAi GIECRTD.
OrthoDBi EOG7W41C4.
TreeFami TF105389.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00319 .
Reactomei REACT_203298. Heme biosynthesis.

Miscellaneous databases

NextBioi 287927.
PROi P22907.
SOURCEi Search...

Gene expression databases

ArrayExpressi P22907.
Bgeei P22907.
CleanExi MM_HMBS.
Genevestigatori P22907.

Family and domain databases

Gene3Di 3.30.160.40. 1 hit.
HAMAPi MF_00260. Porphobil_deam.
InterProi IPR000860. 4pyrrol_synth_OHMeBilane_synth.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view ]
PANTHERi PTHR11557. PTHR11557. 1 hit.
Pfami PF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view ]
PIRSFi PIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSi PR00151. PORPHBDMNASE.
SUPFAMi SSF54782. SSF54782. 2 hits.
TIGRFAMsi TIGR00212. hemC. 1 hit.
PROSITEi PS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse porphobilinogen deaminase gene. Structural organization, sequence, and transcriptional analysis."
    Beaumont C., Porcher C., Picat C., Nordmann Y., Grandchamp B.
    J. Biol. Chem. 264:14829-14834(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Amnion.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-74, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiHEM3_MOUSE
AccessioniPrimary (citable) accession number: P22907
Secondary accession number(s): Q3TIV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The porphobilinogen subunits are added to the dipyrromethane group By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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