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Protein

P34 probable thiol protease

Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable thiol protease.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei200Ancestral active site1
Active sitei301PROSITE-ProRule annotation1
Active sitei321PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Protein family/group databases

MEROPSiI29.003.

Names & Taxonomyi

Protein namesi
Recommended name:
P34 probable thiol protease (EC:3.4.22.-)
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
Proteomesi
  • UP000008827 Componenti: Unplaced

Subcellular locationi

  • Vacuole 1 Publication

  • Note: Protein storage vacuoles of seeds. This protein was wrongly thought to be associated with seed oil bodies.

GO - Cellular componenti

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Protein family/group databases

Allergomei1103. Gly m Bd30K.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
PropeptideiPRO_000002647124 – 122Activation peptide1 PublicationAdd BLAST99
ChainiPRO_0000026472123 – 379P34 probable thiol proteaseAdd BLAST257

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi70N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi190 ↔ 230By similarity
Glycosylationi292N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi293 ↔ 346By similarity

Post-translational modificationi

N-glycosylated on its propeptide.

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Interactioni

Subunit structurei

Homodimer; disulfide-linked.Curated

Protein-protein interaction databases

STRINGi3847.GLYMA08G12270.1.

Structurei

3D structure databases

ProteinModelPortaliP22895.
SMRiP22895.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.

Family and domain databases

InterProiView protein in InterPro
IPR025661. Pept_asp_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiView protein in Pfam
PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
PRINTSiPR00705. PAPAIN.
SMARTiView protein in SMART
SM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
PROSITEiView protein in PROSITE
PS00640. THIOL_PROTEASE_ASN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22895-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLVLLLFS LLGLSSSSSI STHRSILDLD LTKFTTQKQV SSLFQLWKSE
60 70 80 90 100
HGRVYHNHEE EAKRLEIFKN NSNYIRDMNA NRKSPHSHRL GLNKFADITP
110 120 130 140 150
QEFSKKYLQA PKDVSQQIKM ANKKMKKEQY SCDHPPASWD WRKKGVITQV
160 170 180 190 200
KYQGGCGRGW AFSATGAIEA AHAIATGDLV SLSEQELVDC VEESEGSYNG
210 220 230 240 250
WQYQSFEWVL EHGGIATDDD YPYRAKEGRC KANKIQDKVT IDGYETLIMS
260 270 280 290 300
DESTESETEQ AFLSAILEQP ISVSIDAKDF HLYTGGIYDG ENCTSPYGIN
310 320 330 340 350
HFVLLVGYGS ADGVDYWIAK NSWGFDWGED GYIWIQRNTG NLLGVCGMNY
360 370
FASYPTKEES ETLVSARVKG HRRVDHSPL
Length:379
Mass (Da):42,794
Last modified:August 1, 1991 - v1
Checksum:iCC63BA943F3B922D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05560 mRNA. Translation: AAB09252.1. Sequence problems.
PIRiA37126. KHSYO4.
RefSeqiNP_001238219.1. NM_001251290.1.
UniGeneiGma.1816.

Genome annotation databases

GeneIDi548062.
KEGGigmx:548062.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiP34_SOYBN
AccessioniPrimary (citable) accession number: P22895
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 5, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families