Tristetraproline - Mus musculus (Mouse)

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Reviewed, UniProtKB/Swiss-Prot P22893 (TTP_MOUSE)

Last modified June 16, 2009. Version 93. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Tristetraproline
      Short name=TTP
Alternative name(s):
    Zinc finger protein 36
      Short name=Zfp-36
    Protein TIS11A
      Short name=TIS11
    Growth factor-inducible nuclear protein NUP475
    TPA-induced sequence 11

Gene names

Name:	Zfp36
Synonyms:	Tis11, Tis11a

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Probable regulatory protein with a novel zinc finger structure involved in regulating the response to growth factors. Has been experimentally shown to be able to bind zinc.
Subcellular location	Nucleus.
Tissue specificity	Especially abundant in intestine, thymus and regenerating liver, and in macrophage cell line stimulated by gamma-interferon.
Induction	By growth factors and by extracellular signaling agents.
Post-translational modification	Phosphorylated upon mitogen stimulation. Ref.8 Ref.9 Phosphorylated by MAPKAPK2, major sites are Ser-52 and Ser-178. Ref.8 Ref.9
Sequence similarities	Contains 2 C3H1-type zinc fingers.

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Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	RNA destabilization Inferred from mutant phenotype. Source: MGI negative regulation of inflammatory response Inferred from mutant phenotype. Source: MGI negative regulation of myeloid cell differentiation Inferred from mutant phenotype. Source: MGI nuclear-transcribed mRNA poly(A) tail shortening Inferred from direct assay. Source: MGI protein kinase cascade Ref.8 Inferred from direct assay. Source: MGI regulation of mRNA stability Inferred from direct assay. Source: MGI
Cellular component	cytosol Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	AU-rich element binding Inferred from direct assay. Source: MGI DNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA binding Inferred from direct assay. Source: MGI zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 319

319

Tristetraproline

PRO_0000089164

Regions

Repeat

63 – 67

P-P-P-P-G

Repeat

190 – 194

P-P-P-P-G

Repeat

211 – 215

P-P-P-P-G

Zinc finger

95 – 123

C3H1-type 1

Zinc finger

133 – 161

C3H1-type 2

Amino acid modifications

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

178

Phosphoserine Ref.9

Modified residue

220

Phosphoserine; by MAPK; in vitro Ref.8

Modified residue

250

Phosphothreonine

Modified residue

316

Phosphoserine Ref.9

Secondary structure

319

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P22893-1 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 860DD6DDA80386F8
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FASTA

319

33,613

        10         20         30         40         50         60 
MDLSAIYESL QSMSHDLSSD HGGTESLGGL WNINSDSIPS GVTSRLTGRS TSLVEGRSCG 

        70         80         90        100        110        120 
WVPPPPGFAP LAPRPGPELS PSPTSPTATP TTSSRYKTEL CRTYSESGRC RYGAKCQFAH 

       130        140        150        160        170        180 
GLGELRQANR HPKYKTELCH KFYLQGRCPY GSRCHFIHNP TEDLALPGQP HVLRQSISFS 

       190        200        210        220        230        240 
GLPSGRRSSP PPPGFSGPSL SSCSFSPSSS PPPPGDLPLS PSAFSAAPGT PVTRRDPNQA 

       250        260        270        280        290        300 
CCPSCRRSTT PSTIWGPLGG LARSPSAHSL GSDPDDYASS GSSLGGSDSP VFEAGVFGPP 

       310 
QTPAPPRRLP IFNRISVSE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rapid insulin-stimulated accumulation of an mRNA encoding a proline-rich protein." Lai W.S., Stumpo D.J., Blackshear P.J. J. Biol. Chem. 265:16556-16563(1990) [PubMed: 2204625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence." Dubois R.N., McLane M.W., Ryder K., Lau L.F., Nathans D. J. Biol. Chem. 265:19185-19191(1990) [PubMed: 1699942] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Nucleotide sequence of a cDNA encoding TIS11, a message induced in Swiss 3T3 cells by the tumor promoter tetradecanoyl phorbol acetate." Varnum B.C., Lim R.W., Sukhatme V.P., Herschman H.R. Oncogene 4:119-120(1989) [PubMed: 2915901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss.
[4]	"A corrected sequence for the predicted protein from the mitogen-inducible TIS11 primary response gene." Ma Q., Herschman H.R. Oncogene 6:1277-1278(1991) [PubMed: 1861870] [Abstract] Cited for: SEQUENCE REVISION.
[5]	"The TIS11 primary response gene is a member of a gene family that encodes proteins with a highly conserved sequence containing an unusual Cys-His repeat." Varnum B.C., Ma Q., Chi T., Fletcher B.S., Herschman H.R. Mol. Cell. Biol. 11:1754-1758(1991) [PubMed: 1996120] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[6]	"Promoter analysis of Zfp-36, the mitogen-inducible gene encoding the zinc finger protein tristetraprolin." Lai W.S., Thompson M.J., Taylor G.A., Liu Y., Blackshear P.J. J. Biol. Chem. 270:25266-25272(1995) [PubMed: 7559666] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Liver.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
[8]	"Phosphorylation of tristetraprolin, a potential zinc finger transcription factor, by mitogen stimulation in intact cells and by mitogen-activated protein kinase in vitro." Taylor G.A., Thompson M.J., Lai W.S., Blackshear P.J. J. Biol. Chem. 270:13341-13347(1995) [PubMed: 7768935] [Abstract] Cited for: PHOSPHORYLATION AT SER-220.
[9]	"MAPKAP kinase 2 phosphorylates tristetraprolin on in vivo sites including Ser178, a site required for 14-3-3 binding." Chrestensen C.A., Schroeder M.J., Shabanowitz J., Hunt D.F., Pelo J.W., Worthington M.T., Sturgill T.W. J. Biol. Chem. 279:10176-10184(2004) [PubMed: 14688255] [Abstract] Cited for: PHOSPHORYLATION AT SER-52; SER-80; SER-82; SER-85; SER-178; THR-249 AND SER-316, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

M57422 mRNA. Translation: AAA40498.1.
M58691 mRNA. Translation: AAA39837.1.
X14678 mRNA. Translation: CAA32807.1. Sequence problems.
M58565 mRNA. Translation: AAA72947.1.
L42317 Genomic DNA. Translation: AAC37676.1.
BC021391 mRNA. Translation: AAH21391.1.

IPI

IPI00132695.

PIR

A36600.
S04743.

RefSeq

NP_035886.1.

UniGene

Mm.389856

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M9O	NMR	-	A	91-163	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P22893. 1 interaction.

PTM databases

PhosphoSite

P22893.

Genome annotation databases

Ensembl

ENSMUSG00000044786. Mus musculus. [Contig view]

GeneID

22695.

KEGG

mmu:22695.

Organism-specific databases

MGI

MGI:99180. Zfp36.

Phylogenomic databases

HOGENOM

P22893.

HOVERGEN

P22893.

OMA

P22893. NRISVSE.

Gene expression databases

ArrayExpress

P22893.

Bgee

P22893.

CleanEx

MM_ZFP36.

GermOnline

ENSMUSG00000044786. Mus musculus.

Family and domain databases

InterPro

IPR000571. Znf_CCCH.
[Graphical view]

Pfam

PF00642. zf-CCCH. 2 hits.
[Graphical view]

SMART

SM00356. ZnF_C3H1. 2 hits.
[Graphical view]

PROSITE

PS50103. ZF_C3H1. 2 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

303151.

SOURCE

Search...

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Entry information

Entry name

TTP_MOUSE

Accession

Primary (citable) accession number: P22893
Secondary accession number(s): P11520

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 16, 2009
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families