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P22892 (AP1G1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex subunit gamma-1
Alternative name(s):
Adapter-related protein complex 1 subunit gamma-1
Adaptor protein complex AP-1 subunit gamma-1
Clathrin assembly protein complex 1 gamma-1 large chain
Gamma-adaptin
Gamma1-adaptin
Golgi adaptor HA1/AP1 adaptin subunit gamma-1
Gene names
Name:Ap1g1
Synonyms:Adtg, Clapg1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length822 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Subunit structure

Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3) By similarity. Binds RABEP1 By similarity. Binds EPS15 and SYNRG. Interacts (via GAE domain) with AP1AR (via coiled-coil domain) By similarity. Ref.2 Ref.3

Subcellular location

Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the adaptor complexes large subunit family.

Contains 1 GAE domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 822821AP-1 complex subunit gamma-1
PRO_0000193759

Regions

Domain702 – 817116GAE

Amino acid modifications

Modified residue1441N6-acetyllysine By similarity

Experimental info

Mutagenesis7531A → D: Strongly reduces interaction with EPS15 and SYNRG. Ref.2
Mutagenesis7621L → E: Strongly reduces interaction with EPS15 and SYNRG. Ref.2
Mutagenesis7651P → N: Reduces interaction with EPS15 and SYNRG. Ref.2

Secondary structure

1 ......................................................................................................... 822
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22892 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 15317E4BCD9503EB

FASTA82291,350
        10         20         30         40         50         60 
MPAPIRLREL IRTIRTARTQ AEEREMIQKE CAAIRSSFRE EDNTYRCRNV AKLLYMHMLG 

        70         80         90        100        110        120 
YPAHFGQLEC LKLIASQKFT DKRIGYLGAM LLLDERQDVH LLMTNCIKND LNHSTQFVQG 

       130        140        150        160        170        180 
LALCTLGCMG SSEMCRDLAG EVEKLLKTSN SYLRKKAALC AVHVIRKVPE LMEMFLPATK 

       190        200        210        220        230        240 
NLLNEKNHGV LHTSVVLLTE MCERSPDMLA HFRKLVPQLV RILKNLIMSG YSPEHDVSGI 

       250        260        270        280        290        300 
SDPFLQVRIL RLLRILGRND DDSSEAMNDI LAQVATNTET SKNVGNAILY ETVLTIMDIK 

       310        320        330        340        350        360 
SESGLRVLAI NILGRFLLNN DKNIRYVALT SLLKTVQTDH NAVQRHRSTI VDCLKDLDVS 

       370        380        390        400        410        420 
IKRRAMELSF ALVNGNNIRG MMKELLYFLD SCEPEFKADC ASGIFLAAEK YAPSKRWHID 

       430        440        450        460        470        480 
TIMRVLTTAG SYVRDDAVPN LIQLITNSVE MHAYTVQRLY KAILGDYSQQ PLVQVAAWCI 

       490        500        510        520        530        540 
GEYGDLLVSG QCEEEEPIQV TEDEVLDILE SVLISNMSTS VTRGYALTAI MKLSTRFTCT 

       550        560        570        580        590        600 
VNRIKKVVSI YGSSIDVELQ QRAVEYNALF KKYDHMRSAL LERMPVMEKV TTNGPSEIVQ 

       610        620        630        640        650        660 
TNGETEPAPL ETKPPPSGPQ PTSQANDLLD LLGGNDITPV IPTAPTSKPA SAGGELLDLL 

       670        680        690        700        710        720 
GDITLTGAPA AAPTPASVPQ ISQPPFLLDG LSSQPLFNDI APGIPSITAY SKNGLKIEFT 

       730        740        750        760        770        780 
FERSNTNPSV TVITIQASNS TELDMTDFVF QAAVPKTFQL QLLSPSSSVV PAFNTGTITQ 

       790        800        810        820 
VIKVLNPQKQ QLRMRIKLTY NHKGSAMQDL AEVNNFPPQS WQ

« Hide

References

[1]"Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus."
Robinson M.S.
J. Cell Biol. 111:2319-2326(1990) [PubMed: 2126014] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-25.
Tissue: Brain.
[2]"Gamma-adaptin appendage domain: structure and binding site for Eps15 and gamma-synergin."
Kent H.M., McMahon H.T., Evans P.R., Benmerah A., Owen D.J.
Structure 10:1139-1148(2002) [PubMed: 12176391] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.81 ANGSTROMS) OF 704-822, MUTAGENESIS OF ALA-753; LEU-762 AND PRO-765, INTERACTION WITH EPS15 AND SYNRG.
[3]"Crystal structure of the clathrin adaptor protein 1 core."
Heldwein E.E., Macia E., Wang J., Yin H.L., Kirchhausen T., Harrison S.C.
Proc. Natl. Acad. Sci. U.S.A. 101:14108-14113(2004) [PubMed: 15377783] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 1-613, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X54424 mRNA. Translation: CAA38296.1.
IPIIPI00621460.
PIRA36680.
UniGeneMm.37210.
Mm.430782.
Mm.471663.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GYUX-ray1.81A704-822[»]
1GYVX-ray1.71A704-822[»]
1GYWX-ray2.40A/B695-822[»]
1W63X-ray4.00A/C/E/G/I/K1-613[»]
2A7BX-ray1.65A704-821[»]
ProteinModelPortalP22892.
SMRP22892. Positions 1-589, 704-822.
ModBaseSearch...

Protein-protein interaction databases

IntActP22892. 1 interaction.
MINTMINT-200421.
STRINGP22892.

PTM databases

PhosphoSiteP22892.

Proteomic databases

PRIDEP22892.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034171; ENSMUSP00000034171; ENSMUSG00000031731.
UCSCuc012gkl.1. mouse.

Organism-specific databases

MGIMGI:101919. Ap1g1.

Phylogenomic databases

eggNOGroNOG11683.
HOVERGENHBG067473.
OrthoDBEOG4ZGPBP.
PhylomeDBP22892.

Gene expression databases

ArrayExpressP22892.
BgeeP22892.
CleanExMM_AP1G1.
GenevestigatorP22892.

Family and domain databases

InterProIPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
Gene3DG3DSA:1.25.10.10. ARM-like. 1 hit.
G3DSA:2.60.40.1230. Clathrin_g-adaptin_app. 1 hit.
PfamPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]
PIRSFPIRSF037094. AP1_complex_gamma. 1 hit.
SMARTSM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]
SUPFAMSSF48371. ARM-type_fold. 1 hit.
SSF49348. Clath_adapt. 1 hit.
PROSITEPS50180. GAE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

SOURCESearch...

Entry information

Entry nameAP1G1_MOUSE
AccessionPrimary (citable) accession number: P22892
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: December 14, 2011
This is version 107 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents