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Protein

AP-1 complex subunit gamma-1

Gene

Ap1g1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit gamma-1
Alternative name(s):
Adaptor protein complex AP-1 subunit gamma-1
Adaptor-related protein complex 1 subunit gamma-1
Clathrin assembly protein complex 1 gamma-1 large chain
Gamma-adaptin
Gamma1-adaptin
Golgi adaptor HA1/AP1 adaptin subunit gamma-1
Gene namesi
Name:Ap1g1
Synonyms:Adtg, Clapg1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:101919. Ap1g1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi753A → D: Strongly reduces interaction with EPS15 and SYNRG. 1 Publication1
Mutagenesisi762L → E: Strongly reduces interaction with EPS15 and SYNRG. 1 Publication1
Mutagenesisi765P → N: Reduces interaction with EPS15 and SYNRG. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001937592 – 822AP-1 complex subunit gamma-1Add BLAST821

Proteomic databases

EPDiP22892.
MaxQBiP22892.
PaxDbiP22892.
PeptideAtlasiP22892.
PRIDEiP22892.

PTM databases

iPTMnetiP22892.
PhosphoSitePlusiP22892.
SwissPalmiP22892.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiENSMUSG00000031731.
CleanExiMM_AP1G1.
ExpressionAtlasiP22892. baseline and differential.
GenevisibleiP22892. MM.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3) (By similarity). Binds RABEP1 (By similarity). Binds EPS15 and SYNRG. Interacts (via GAE domain) with AP1AR (via coiled-coil domain) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Ap1m1P355856EBI-1040262,EBI-1040251
CLINT1Q1467710EBI-1040262,EBI-1171113From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198123. 3 interactors.
IntActiP22892. 18 interactors.
MINTiMINT-200421.
STRINGi10090.ENSMUSP00000090844.

Structurei

Secondary structure

1822
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 15Combined sources9
Helixi20 – 39Combined sources20
Beta strandi43 – 45Combined sources3
Helixi46 – 58Combined sources13
Helixi64 – 66Combined sources3
Helixi67 – 74Combined sources8
Beta strandi76 – 78Combined sources3
Helixi79 – 92Combined sources14
Helixi100 – 111Combined sources12
Helixi116 – 129Combined sources14
Helixi132 – 146Combined sources15
Helixi151 – 167Combined sources17
Helixi170 – 175Combined sources6
Helixi176 – 179Combined sources4
Helixi188 – 204Combined sources17
Helixi206 – 212Combined sources7
Helixi216 – 228Combined sources13
Turni233 – 235Combined sources3
Helixi243 – 255Combined sources13
Turni256 – 259Combined sources4
Helixi262 – 277Combined sources16
Helixi283 – 298Combined sources16
Helixi303 – 317Combined sources15
Helixi322 – 334Combined sources13
Beta strandi336 – 339Combined sources4
Helixi340 – 343Combined sources4
Helixi347 – 352Combined sources6
Helixi353 – 355Combined sources3
Helixi359 – 370Combined sources12
Turni375 – 377Combined sources3
Helixi378 – 390Combined sources13
Helixi394 – 410Combined sources17
Helixi415 – 428Combined sources14
Helixi430 – 432Combined sources3
Helixi435 – 437Combined sources3
Helixi438 – 446Combined sources9
Turni449 – 452Combined sources4
Helixi453 – 465Combined sources13
Helixi470 – 482Combined sources13
Helixi484 – 488Combined sources5
Helixi503 – 514Combined sources12
Helixi520 – 536Combined sources17
Helixi541 – 550Combined sources10
Helixi551 – 553Combined sources3
Helixi557 – 572Combined sources16
Helixi576 – 580Combined sources5
Beta strandi707 – 712Combined sources6
Beta strandi715 – 722Combined sources8
Beta strandi730 – 739Combined sources10
Beta strandi741 – 743Combined sources3
Beta strandi745 – 753Combined sources9
Beta strandi758 – 762Combined sources5
Helixi772 – 774Combined sources3
Beta strandi778 – 785Combined sources8
Beta strandi794 – 802Combined sources9
Beta strandi805 – 813Combined sources9
Helixi818 – 820Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GYUX-ray1.81A704-822[»]
1GYVX-ray1.71A704-822[»]
1GYWX-ray2.40A/B695-822[»]
1W63X-ray4.00A/C/E/G/I/K1-613[»]
2A7BX-ray1.65A704-822[»]
3ZY7X-ray1.09A/B704-822[»]
4HMYX-ray7.00A1-595[»]
4P6ZX-ray3.00G1-613[»]
ProteinModelPortaliP22892.
SMRiP22892.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22892.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini702 – 817GAEPROSITE-ProRule annotationAdd BLAST116

Sequence similaritiesi

Contains 1 GAE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1062. Eukaryota.
ENOG410XPKK. LUCA.
GeneTreeiENSGT00390000012618.
HOGENOMiHOG000210271.
HOVERGENiHBG067473.
InParanoidiP22892.
KOiK12391.
PhylomeDBiP22892.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1230. 2 hits.
InterProiIPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]
PIRSFiPIRSF037094. AP1_complex_gamma. 1 hit.
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22892-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAPIRLREL IRTIRTARTQ AEEREMIQKE CAAIRSSFRE EDNTYRCRNV
60 70 80 90 100
AKLLYMHMLG YPAHFGQLEC LKLIASQKFT DKRIGYLGAM LLLDERQDVH
110 120 130 140 150
LLMTNCIKND LNHSTQFVQG LALCTLGCMG SSEMCRDLAG EVEKLLKTSN
160 170 180 190 200
SYLRKKAALC AVHVIRKVPE LMEMFLPATK NLLNEKNHGV LHTSVVLLTE
210 220 230 240 250
MCERSPDMLA HFRKLVPQLV RILKNLIMSG YSPEHDVSGI SDPFLQVRIL
260 270 280 290 300
RLLRILGRND DDSSEAMNDI LAQVATNTET SKNVGNAILY ETVLTIMDIK
310 320 330 340 350
SESGLRVLAI NILGRFLLNN DKNIRYVALT SLLKTVQTDH NAVQRHRSTI
360 370 380 390 400
VDCLKDLDVS IKRRAMELSF ALVNGNNIRG MMKELLYFLD SCEPEFKADC
410 420 430 440 450
ASGIFLAAEK YAPSKRWHID TIMRVLTTAG SYVRDDAVPN LIQLITNSVE
460 470 480 490 500
MHAYTVQRLY KAILGDYSQQ PLVQVAAWCI GEYGDLLVSG QCEEEEPIQV
510 520 530 540 550
TEDEVLDILE SVLISNMSTS VTRGYALTAI MKLSTRFTCT VNRIKKVVSI
560 570 580 590 600
YGSSIDVELQ QRAVEYNALF KKYDHMRSAL LERMPVMEKV TTNGPSEIVQ
610 620 630 640 650
TNGETEPAPL ETKPPPSGPQ PTSQANDLLD LLGGNDITPV IPTAPTSKPA
660 670 680 690 700
SAGGELLDLL GDITLTGAPA AAPTPASVPQ ISQPPFLLDG LSSQPLFNDI
710 720 730 740 750
APGIPSITAY SKNGLKIEFT FERSNTNPSV TVITIQASNS TELDMTDFVF
760 770 780 790 800
QAAVPKTFQL QLLSPSSSVV PAFNTGTITQ VIKVLNPQKQ QLRMRIKLTY
810 820
NHKGSAMQDL AEVNNFPPQS WQ
Length:822
Mass (Da):91,350
Last modified:January 23, 2007 - v3
Checksum:i15317E4BCD9503EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54424 mRNA. Translation: CAA38296.1.
CCDSiCCDS80929.1.
PIRiA36680.
RefSeqiNP_001288140.1. NM_001301211.1.
UniGeneiMm.37210.
Mm.486894.

Genome annotation databases

EnsembliENSMUST00000034171; ENSMUSP00000034171; ENSMUSG00000031731.
GeneIDi11765.
KEGGimmu:11765.
UCSCiuc012gkl.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54424 mRNA. Translation: CAA38296.1.
CCDSiCCDS80929.1.
PIRiA36680.
RefSeqiNP_001288140.1. NM_001301211.1.
UniGeneiMm.37210.
Mm.486894.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GYUX-ray1.81A704-822[»]
1GYVX-ray1.71A704-822[»]
1GYWX-ray2.40A/B695-822[»]
1W63X-ray4.00A/C/E/G/I/K1-613[»]
2A7BX-ray1.65A704-822[»]
3ZY7X-ray1.09A/B704-822[»]
4HMYX-ray7.00A1-595[»]
4P6ZX-ray3.00G1-613[»]
ProteinModelPortaliP22892.
SMRiP22892.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198123. 3 interactors.
IntActiP22892. 18 interactors.
MINTiMINT-200421.
STRINGi10090.ENSMUSP00000090844.

PTM databases

iPTMnetiP22892.
PhosphoSitePlusiP22892.
SwissPalmiP22892.

Proteomic databases

EPDiP22892.
MaxQBiP22892.
PaxDbiP22892.
PeptideAtlasiP22892.
PRIDEiP22892.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034171; ENSMUSP00000034171; ENSMUSG00000031731.
GeneIDi11765.
KEGGimmu:11765.
UCSCiuc012gkl.3. mouse.

Organism-specific databases

CTDi164.
MGIiMGI:101919. Ap1g1.

Phylogenomic databases

eggNOGiKOG1062. Eukaryota.
ENOG410XPKK. LUCA.
GeneTreeiENSGT00390000012618.
HOGENOMiHOG000210271.
HOVERGENiHBG067473.
InParanoidiP22892.
KOiK12391.
PhylomeDBiP22892.

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-432720. Lysosome Vesicle Biogenesis.
R-MMU-432722. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

EvolutionaryTraceiP22892.
PROiP22892.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031731.
CleanExiMM_AP1G1.
ExpressionAtlasiP22892. baseline and differential.
GenevisibleiP22892. MM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1230. 2 hits.
InterProiIPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]
PIRSFiPIRSF037094. AP1_complex_gamma. 1 hit.
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAP1G1_MOUSE
AccessioniPrimary (citable) accession number: P22892
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.