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Protein

Glutamine synthetase

Gene

glnB

Organism
Streptomyces hygroscopicus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Miscellaneous

Two forms of glutamine synthetase (GSI and GSII) can be found in this bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi109MagnesiumBy similarity1
Metal bindingi111MagnesiumBy similarity1
Metal bindingi171MagnesiumBy similarity1
Metal bindingi178MagnesiumBy similarity1
Binding sitei276L-glutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase1 Publication (EC:6.3.1.2By similarity)
Short name:
GS1 Publication
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase II1 Publication
Short name:
GSII1 Publication
Gene namesi
Name:glnB1 Publication
OrganismiStreptomyces hygroscopicus
Taxonomic identifieri1912 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001532672 – 340Glutamine synthetaseAdd BLAST339

Interactioni

Subunit structurei

Homooctamer and homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP22878.
SMRiP22878.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22878-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIKAEYIWI DGTQPTAKLR SKTKILSDGS RLPRWGFDGS STNQAEGHAS
60 70 80 90 100
DLVLEPVFSC PDPIRGGDHL LVLCEVLHTD LTPHPSNTRA LLRPVAERFA
110 120 130 140 150
GQEPIFGIEQ EYTFLKGDRP LGFPEGGGYP APQADYYCGV GADAIFGREI
160 170 180 190 200
VEKHLDLCLA AGLGLSGINA EVMPGQWEFQ VGALPPLEVS DHMWVARWLL
210 220 230 240 250
HRVAEEFGVT ASLDAKPAKG DWNGAGAHTN FSTRAMREGY DPIITACEAL
260 270 280 290 300
GQDDKPLEHV RQYGTGIEDR LTGAHETAPW DAYSYGASDR GASVRIPWQV
310 320 330 340
EVEKKGYIED RRPNANVDPY VVTRLMVDTC CTELARREQI
Length:340
Mass (Da):37,417
Last modified:August 1, 1991 - v1
Checksum:iCD883389BB979C37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33783 Genomic DNA. Translation: AAA26749.1.
PIRiA36726. AJSM2H.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33783 Genomic DNA. Translation: AAA26749.1.
PIRiA36726. AJSM2H.

3D structure databases

ProteinModelPortaliP22878.
SMRiP22878.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLNA2_STRHY
AccessioniPrimary (citable) accession number: P22878
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 12, 2017
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.