P22873 (CRTE_ESCVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Geranylgeranyl pyrophosphate synthase
Short name=GGPP synthase
EC=2.5.1.29

Alternative name(s):
Farnesyltranstransferase

Gene names

Name:

crtE

Organism

Escherichia vulneris

Taxonomic identifier

566 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1.
Sequence similarities	Belongs to the FPP/GGPP synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Isoprene biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Gene Ontology (GO)
Biological process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	farnesyltranstransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Geranylgeranyl pyrophosphate synthase		PRO_0000123992
Sites
	Metal binding	93	1	Magnesium 1 By similarity
	Metal binding	93	1	Magnesium 2 By similarity
	Metal binding	99	1	Magnesium 1 By similarity
	Metal binding	99	1	Magnesium 2 By similarity
	Metal binding	229	1	Magnesium 3 By similarity
	Binding site	52	1	Isopentenyl diphosphate By similarity
	Binding site	55	1	Isopentenyl diphosphate By similarity
	Binding site	86	1	Isopentenyl diphosphate By similarity
	Binding site	104	1	Farnesyl diphosphate By similarity
	Binding site	105	1	Isopentenyl diphosphate By similarity
	Binding site	188	1	Farnesyl diphosphate By similarity
	Binding site	189	1	Farnesyl diphosphate By similarity
	Binding site	226	1	Farnesyl diphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22873 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 6A534C6194CE9F59	FASTA	307	33,242
	10 20 30 40 50 60 MVSGSKAGVS PHREIEVMRQ SIDDHLAGLL PETDSQDIVS LAMREGVMAP GKRIRPLLML 70 80 90 100 110 120 LAARDLRYQG SMPTLLDLAC AVELTHTASL MLDDMPCMDN AELRRGQPTT HKKFGESVAI 130 140 150 160 170 180 LASVGLLSKA FGLIAATGDL PGERRAQAVN ELSTAVGVQG LVLGQFRDLN DAALDRTPDA 190 200 210 220 230 240 ILSTNHLKTG ILFSAMLQIV AIASASSPST RETLHAFALD FGQAFQLLDD LRDDHPETGK 250 260 270 280 290 300 DRNKDAGKST LVNRLGADAA RQKLREHIDS ADKHLTFACP QGGAIRQFMH LWFGHHLADW SPVMKIA

References

[1]	"Conserved enzymes mediate the early reactions of carotenoid biosynthesis in nonphotosynthetic and photosynthetic prokaryotes." Armstrong G.A., Alberti M., Hearst J.E. Proc. Natl. Acad. Sci. U.S.A. 87:9975-9979(1990) [PubMed: 2263648] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 39368 / Eho10.
[2]	"The crtE gene in Erwinia herbicola encodes geranylgeranyl diphosphate synthase." Math S.K., Hearst J.E., Poulter C.D. Proc. Natl. Acad. Sci. U.S.A. 89:6761-6764(1992) [PubMed: 1495965] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38424 Genomic DNA. Translation: AAA24819.1. M87280 Genomic DNA. Translation: AAA64977.1.
PIR	C39273.
3D structure databases
ProteinModelPortal	P22873.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
PROSITE	PS00723. POLYPRENYL_SYNTHASE_1. 1 hit. PS00444. POLYPRENYL_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRTE_ESCVU

Accession

Primary (citable) accession number: P22873

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	September 21, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program