P22872 (CRTB_ESCVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phytoene synthase
EC=2.5.1.32

Gene names

Name:

crtB

Organism

Escherichia vulneris

Taxonomic identifier

566 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reaction from prephytoene diphosphate to phytoene. Ref.2
Catalytic activity	2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Sequence similarities	Belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Molecular function	Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Phytoene synthase		PRO_0000067428

Sequences

	Sequence		Length	Mass (Da)
	P22872 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: B64235C9E0C06F38	FASTA	309	34,123
	10 20 30 40 50 60 MSQPPLLDHA TQTMANGSKS FATAAKLFDP ATRRSVLMLY TWCRHCDDVI DDQTHGFASE 70 80 90 100 110 120 AAAEEEATQR LARLRTLTLA AFEGAEMQDP AFAAFQEVAL THGITPRMAL DHLDGFAMDV 130 140 150 160 170 180 AQTRYVTFED TLRYCYHVAG VVGLMMARVM GVRDERVLDR ACDLGLAFQL TNIARDIIDD 190 200 210 220 230 240 AAIDRCYLPA EWLQDAGLTP ENYAARENRA ALARVAERLI DAAEPYYISS QAGLHDLPPR 250 260 270 280 290 300 CAWAIATARS VYREIGIKVK AAGGSAWDRR QHTSKGEKIA MLMAAPGQVI RAKTTRVTPR PAGLWQRPV

References

[1]	"Conserved enzymes mediate the early reactions of carotenoid biosynthesis in nonphotosynthetic and photosynthetic prokaryotes." Armstrong G.A., Alberti M., Hearst J.E. Proc. Natl. Acad. Sci. U.S.A. 87:9975-9979(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 39368 / Eho10.
[2]	"The crtE gene in Erwinia herbicola encodes geranylgeranyl diphosphate synthase." Math S.K., Hearst J.E., Poulter C.D. Proc. Natl. Acad. Sci. U.S.A. 89:6761-6764(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38423 Genomic DNA. Translation: AAA24821.1. M87280 Genomic DNA. Translation: AAA64982.1.
PIR	B39273.
3D structure databases
ProteinModelPortal	P22872.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00799; UER00773.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
PROSITE	PS01044. SQUALEN_PHYTOEN_SYN_1. 1 hit. PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRTB_ESCVU

Accession

Primary (citable) accession number: P22872

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program