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Protein

Methane monooxygenase component C

Gene

mmoC

Organism
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the initial oxygenation of methane to methanol in methanotrophs. It also catalyzes the monohydroxylation of a variety of unactivated alkenes, alicyclic, aromatic and heterocyclic compounds. The component C is the iron-sulfur flavoprotein of sMMO.

Catalytic activityi

Methane + NAD(P)H + O2 = methanol + NAD(P)+ + H2O.

Cofactori

[2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42Iron-sulfur (2Fe-2S)1
Metal bindingi47Iron-sulfur (2Fe-2S)1
Metal bindingi50Iron-sulfur (2Fe-2S)1
Metal bindingi82Iron-sulfur (2Fe-2S)1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 235FADBy similarityAdd BLAST15

GO - Molecular functioni

GO - Biological processi

  • methane metabolic process Source: JCVI
  • one-carbon metabolic process Source: UniProtKB-KW
  • oxidation-reduction process Source: JCVI

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processElectron transport, One-carbon metabolism, Transport
Ligand2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMCAP243233:G1G0X-1200-MONOMER
MetaCyc:MONOMER-3864

Names & Taxonomyi

Protein namesi
Recommended name:
Methane monooxygenase component C (EC:1.14.13.25)
Alternative name(s):
Methane hydroxylase
Methane monooxygenase reductase
Short name:
MMOR
Gene namesi
Name:mmoC
Ordered Locus Names:MCA1200
OrganismiMethylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Taxonomic identifieri243233 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaMethylococcalesMethylococcaceaeMethylococcus
Proteomesi
  • UP000006821 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  • methane monooxygenase complex Source: JCVI

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001894081 – 348Methane monooxygenase component CAdd BLAST348

Interactioni

Subunit structurei

The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD).

Protein-protein interaction databases

STRINGi243233.MCA1200

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 11Combined sources8
Turni12 – 14Combined sources3
Beta strandi15 – 23Combined sources9
Helixi27 – 34Combined sources8
Beta strandi54 – 56Combined sources3
Turni66 – 68Combined sources3
Helixi71 – 77Combined sources7
Turni81 – 83Combined sources3
Beta strandi90 – 93Combined sources4
Beta strandi106 – 113Combined sources8
Beta strandi117 – 120Combined sources4
Beta strandi123 – 128Combined sources6
Beta strandi134 – 138Combined sources5
Beta strandi147 – 150Combined sources4
Beta strandi156 – 161Combined sources6
Beta strandi167 – 170Combined sources4
Beta strandi173 – 177Combined sources5
Beta strandi183 – 185Combined sources3
Helixi186 – 192Combined sources7
Beta strandi193 – 205Combined sources13
Beta strandi214 – 216Combined sources3
Beta strandi218 – 225Combined sources8
Helixi228 – 240Combined sources13
Beta strandi246 – 251Combined sources6
Beta strandi253 – 255Combined sources3
Helixi261 – 270Combined sources10
Beta strandi271 – 273Combined sources3
Beta strandi275 – 278Combined sources4
Beta strandi288 – 295Combined sources8
Helixi296 – 304Combined sources9
Beta strandi305 – 316Combined sources12
Helixi317 – 330Combined sources14
Beta strandi335 – 339Combined sources5
Beta strandi343 – 345Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JQ4NMR-A1-98[»]
1TVCNMR-A99-348[»]
DisProtiDP00379
ProteinModelPortaliP22868
SMRiP22868
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22868

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 982Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST94
Domaini106 – 211FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST106

Phylogenomic databases

eggNOGiENOG4107TVJ Bacteria
COG0543 LUCA
HOGENOMiHOG000263663
KOiK16161
OMAiDEIRFEA
OrthoDBiPOG091H05I4

Family and domain databases

CDDicd00207 fer2, 1 hit
Gene3Di3.10.20.30, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR012675 Beta-grasp_dom_sf
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR001221 Phe_hydroxylase
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00111 Fer2, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00371 FPNCR
PR00410 PHEHYDRXLASE
SUPFAMiSSF54292 SSF54292, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

P22868-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRVHTITAV TEDGESLRFE CRSDEDVITA ALRQNIFLMS SCREGGCATC
60 70 80 90 100
KALCSEGDYD LKGCSVQALP PEEEEEGLVL LCRTYPKTDL EIELPYTHCR
110 120 130 140 150
ISFGEVGSFE AEVVGLNWVS SNTVQFLLQK RPDECGNRGV KFEPGQFMDL
160 170 180 190 200
TIPGTDVSRS YSPANLPNPE GRLEFLIRVL PEGRFSDYLR NDARVGQVLS
210 220 230 240 250
VKGPLGVFGL KERGMAPRYF VAGGTGLAPV VSMVRQMQEW TAPNETRIYF
260 270 280 290 300
GVNTEPELFY IDELKSLERS MRNLTVKACV WHPSGDWEGE QGSPIDALRE
310 320 330 340
DLESSDANPD IYLCGPPGMI DAACELVRSR GIPGEQVFFE KFLPSGAA
Length:348
Mass (Da):38,542
Last modified:September 26, 2001 - v2
Checksum:i7577BEB408CA1C1F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M90050 Genomic DNA Translation: AAB62391.2
AE017282 Genomic DNA Translation: AAU92722.1
PIRiJQ0701
RefSeqiWP_010960487.1, NC_002977.6

Genome annotation databases

EnsemblBacteriaiAAU92722; AAU92722; MCA1200
KEGGimca:MCA1200

Similar proteinsi

Entry informationi

Entry nameiMMOC_METCA
AccessioniPrimary (citable) accession number: P22868
Secondary accession number(s): Q609N2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: September 26, 2001
Last modified: March 28, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health