Reviewed, UniProtKB/Swiss-Prot P22864 (PLYD_ASPNG)

Names and origin

Protein names

Recommended name:
    Pectin lyase D
      Short name=PLD
    EC=4.2.2.10
Alternative name(s):
    Pectin lyase I
      Short name=PLI

Gene names

Name:

pelD

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Post-translational modification	May be O-glycosylated; does not contain N-acetylglucosamine.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Lyase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	pectin lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.1
	Chain	20 – 373	354	Pectin lyase D		PRO_0000024898
Sites
	Active site	255	1	Potential
Amino acid modifications
	Glycosylation	128	1	N-linked (GlcNAc...) Potential
	Glycosylation	274	1	N-linked (GlcNAc...) Potential
	Glycosylation	348	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	27	1	V → E AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22864-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: B758DCCEFFE2345C	FASTA	373	39,038
	10 20 30 40 50 60 MKYAAALTAI AALAARAAAV GVSGTPVGFA SSATGGGDAT PVYPTTTDEL VSYLGDDEAR 70 80 90 100 110 120 VIVLSKTFDF TDTEGTTTTT GCAPWGTASG CQLAINKDDW CTNYEPDAPT TTVTYNTAGE 130 140 150 160 170 180 LGITVNSNKS LIGEGTSGVI KGRGLRMVSG VSNIIIQNIA VTDINPEYVW GGDAITLDEA 190 200 210 220 230 240 DLVWIDHVTT ARIGRQHYVL GTDADSRVSI TNNYINGESD YSATCDGHHY WNVYLDGSSD 250 260 270 280 290 300 KVTFSGNYLY KTSGRAPKVQ DNTYLHIYNN YWENNSGHAF EIGSGGYVLA EGNYFSNVDT 310 320 330 340 350 360 VLETDTFEGA LFSSDSASST CESYIGRSCV ANVNGGDLTG TSTTVLSNLS GDTLPSADAA 370 STSPASNAGQ GNL

References

[1]	"Isolation and structure of the pectin lyase D-encoding gene from Aspergillus niger." Gysler C., Harmsen J.A.M., Kester H.C.M., Visser J., Heim J. Gene 89:101-108(1990) [PubMed: 2373363] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 20-30 AND 148-167. Strain: N756.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M55657 Genomic DNA. Translation: AAA32701.1.
PIR	JH0155.
3D structure databases
HSSP	HSSP built from PDB template 1IDK based on UniProtKB Q01172.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Enzyme and pathway databases
BRENDA	4.2.2.10. 277.
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLYD_ASPNG

Accession

Primary (citable) accession number: P22864

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	April 14, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)