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P22861

- AMYG_SCHOC

UniProt

P22861 - AMYG_SCHOC

Protein

Glucoamylase 1

Gene

GAM1

Organism
Schwanniomyces occidentalis (Yeast) (Debaryomyces occidentalis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    This glucoamylase has a specificity toward both alpha-1,4 and alpha-1,6 linkages.

    Catalytic activityi

    Hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.PROSITE-ProRule annotation
    Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei470 – 4701By similarity
    Active sitei473 – 4731By similarity
    Active sitei638 – 6381Proton donorBy similarity

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH31. Glycoside Hydrolase Family 31.
    mycoCLAPiGLA31A_DEBOC.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucoamylase 1 (EC:3.2.1.3)
    Alternative name(s):
    1,4-alpha-D-glucan glucohydrolase
    Glucan 1,4-alpha-glucosidase
    Gene namesi
    Name:GAM1
    OrganismiSchwanniomyces occidentalis (Yeast) (Debaryomyces occidentalis)
    Taxonomic identifieri27300 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeSchwanniomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 958936Glucoamylase 1PRO_0000018586Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi416 – 4161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi580 – 5801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi602 – 6021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi813 – 8131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi907 – 9071N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi26 – 4116Ser-richAdd
    BLAST
    Compositional biasi530 – 54213Ser/Thr-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 31 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF01055. Glyco_hydro_31. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
    PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22861-1 [UniParc]FASTAAdd to Basket

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    MIFLKLIKSI VIGLGLVSAI QAAPASSIGS SASASSSSES SQATIPNDVT    50
    LGVKQIPNIF NDSAVDANAA AKGYDLVNVT NTPRGLTGIL KLKEATNIYG 100
    YDFDYLNLTV EYQADTRLNV HIEPTDLSDV FVLPEHLVVK PLVEGDAQSY 150
    NFDNSDLVFE YSNTDFSFEV IRSSTKEVLF STKGNPLVFS NQFIQFNSSL 200
    PKNHVITGLG ESIHGLVNEP GSVKTLFAND VGDPIDGNIY GVHPVYLDQR 250
    YDTETTHAVY WRTSAIQEVL IGEESITWRA LSGVIDLYFF SGPTPKDAIQ 300
    QYVKEIGLPA FQPYWSLGYH QCRWGYDTIE KLSEVVENFK KFNIPLETIW 350
    SDIDYMDSYK DFTYDPHRFP LDEYRKFLDE LHKNNQHYVP ILDAAIYVPN 400
    PNNATDNEYQ PFHYGNETDV FLKNPDGSLY IGAVWQVTLF SRFLSRKHSD 450
    MDKVIKDWYE LTPFDGIWAD MNEVSSFCVG SCGTGKYFEN PAYPPFTVGS 500
    KATSYPVGFD VSNASEWKSI QSSISATAKT SSTSSVSSSS STIDYMNTLA 550
    PGKGNINYPP YAIYNMQGDS DLATHAVSPN ATHADGTVEY DIHNLYGYLQ 600
    ENATYHALLE VFPNKRPFMI SRSTFPRAGK WTGHWGGDNT ADWAYAYFSI 650
    PQAFSMGIAG LPFFGADVCG FNGNSDSELC SRWMQLGSFF PFYRNHNYLG 700
    AIDQEPYVWE SVAEATRTSM AIRYLLLPYY YTLLHESHTT GLPILRAFSW 750
    QFPNDRSLSG VDNQFFVGDG LVVTPVLEPG VDKVKGVFPG AGKEEVYYDW 800
    YTQREVHFKD GKNETLDAPL GHIPLHIRGG NVLPTQEPGY TVAESRQNPF 850
    GLIVALDNDG KAQGSLYLDD GESLVVDSSL LVSFSVSDNT LSASPSGDYK 900
    ADQPLANVTI LGVGHKPKSV KFENANVDFT YKKSTVFVTG LDKYTKDGAF 950
    SKDFTITW 958
    Length:958
    Mass (Da):106,508
    Last modified:August 1, 1991 - v1
    Checksum:i47938DB9BC308260
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60207 Genomic DNA. Translation: AAA33923.1.
    PIRiJN0102.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60207 Genomic DNA. Translation: AAA33923.1 .
    PIRi JN0102.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH31. Glycoside Hydrolase Family 31.
    mycoCLAPi GLA31A_DEBOC.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF01055. Glyco_hydro_31. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
    PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the Schwanniomyces occidentalis glucoamylase gene (GAM1) and its expression in Saccharomyces cerevisiae."
      Dohmen R.J., Strasser A.W.M., Dahlems U.M., Hollenberg C.P.
      Gene 95:111-121(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 26076 / DSM 3794 / CBS 2864 / BCRC 22059 / NCYC 954 / NRRL Y-2470.
    2. "Striking structural and functional similarities suggest that intestinal sucrase-isomaltase, human lysosomal alpha-glucosidase and Schwanniomyces occidentalis glucoamylase are derived from a common ancestral gene."
      Naim H.Y., Niermann T., Kleinhans U., Hollenberg C.P., Strasser A.W.M.
      FEBS Lett. 294:109-112(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIMILARITY TO OTHER FAMILY 31 MEMBERS.

    Entry informationi

    Entry nameiAMYG_SCHOC
    AccessioniPrimary (citable) accession number: P22861
    Secondary accession number(s): Q92336
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 57 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3