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P22861

- AMYG_SCHOC

UniProt

P22861 - AMYG_SCHOC

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Protein

Glucoamylase 1

Gene
GAM1
Organism
Schwanniomyces occidentalis (Yeast) (Debaryomyces occidentalis)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

This glucoamylase has a specificity toward both alpha-1,4 and alpha-1,6 linkages.

Catalytic activityi

Hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei470 – 4701 By similarity
Active sitei473 – 4731 By similarity
Active sitei638 – 6381Proton donor By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.
mycoCLAPiGLA31A_DEBOC.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase 1 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:GAM1
OrganismiSchwanniomyces occidentalis (Yeast) (Debaryomyces occidentalis)
Taxonomic identifieri27300 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeSchwanniomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed predictionAdd
BLAST
Chaini23 – 958936Glucoamylase 1PRO_0000018586Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...) Reviewed prediction
Glycosylationi78 – 781N-linked (GlcNAc...) Reviewed prediction
Glycosylationi107 – 1071N-linked (GlcNAc...) Reviewed prediction
Glycosylationi197 – 1971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi403 – 4031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi416 – 4161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi513 – 5131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi580 – 5801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi602 – 6021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi813 – 8131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi907 – 9071N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 4116Ser-richAdd
BLAST
Compositional biasi530 – 54213Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22861-1 [UniParc]FASTAAdd to Basket

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MIFLKLIKSI VIGLGLVSAI QAAPASSIGS SASASSSSES SQATIPNDVT    50
LGVKQIPNIF NDSAVDANAA AKGYDLVNVT NTPRGLTGIL KLKEATNIYG 100
YDFDYLNLTV EYQADTRLNV HIEPTDLSDV FVLPEHLVVK PLVEGDAQSY 150
NFDNSDLVFE YSNTDFSFEV IRSSTKEVLF STKGNPLVFS NQFIQFNSSL 200
PKNHVITGLG ESIHGLVNEP GSVKTLFAND VGDPIDGNIY GVHPVYLDQR 250
YDTETTHAVY WRTSAIQEVL IGEESITWRA LSGVIDLYFF SGPTPKDAIQ 300
QYVKEIGLPA FQPYWSLGYH QCRWGYDTIE KLSEVVENFK KFNIPLETIW 350
SDIDYMDSYK DFTYDPHRFP LDEYRKFLDE LHKNNQHYVP ILDAAIYVPN 400
PNNATDNEYQ PFHYGNETDV FLKNPDGSLY IGAVWQVTLF SRFLSRKHSD 450
MDKVIKDWYE LTPFDGIWAD MNEVSSFCVG SCGTGKYFEN PAYPPFTVGS 500
KATSYPVGFD VSNASEWKSI QSSISATAKT SSTSSVSSSS STIDYMNTLA 550
PGKGNINYPP YAIYNMQGDS DLATHAVSPN ATHADGTVEY DIHNLYGYLQ 600
ENATYHALLE VFPNKRPFMI SRSTFPRAGK WTGHWGGDNT ADWAYAYFSI 650
PQAFSMGIAG LPFFGADVCG FNGNSDSELC SRWMQLGSFF PFYRNHNYLG 700
AIDQEPYVWE SVAEATRTSM AIRYLLLPYY YTLLHESHTT GLPILRAFSW 750
QFPNDRSLSG VDNQFFVGDG LVVTPVLEPG VDKVKGVFPG AGKEEVYYDW 800
YTQREVHFKD GKNETLDAPL GHIPLHIRGG NVLPTQEPGY TVAESRQNPF 850
GLIVALDNDG KAQGSLYLDD GESLVVDSSL LVSFSVSDNT LSASPSGDYK 900
ADQPLANVTI LGVGHKPKSV KFENANVDFT YKKSTVFVTG LDKYTKDGAF 950
SKDFTITW 958
Length:958
Mass (Da):106,508
Last modified:August 1, 1991 - v1
Checksum:i47938DB9BC308260
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60207 Genomic DNA. Translation: AAA33923.1.
PIRiJN0102.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60207 Genomic DNA. Translation: AAA33923.1 .
PIRi JN0102.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.
mycoCLAPi GLA31A_DEBOC.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01055. Glyco_hydro_31. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of the Schwanniomyces occidentalis glucoamylase gene (GAM1) and its expression in Saccharomyces cerevisiae."
    Dohmen R.J., Strasser A.W.M., Dahlems U.M., Hollenberg C.P.
    Gene 95:111-121(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 26076 / DSM 3794 / CBS 2864 / BCRC 22059 / NCYC 954 / NRRL Y-2470.
  2. "Striking structural and functional similarities suggest that intestinal sucrase-isomaltase, human lysosomal alpha-glucosidase and Schwanniomyces occidentalis glucoamylase are derived from a common ancestral gene."
    Naim H.Y., Niermann T., Kleinhans U., Hollenberg C.P., Strasser A.W.M.
    FEBS Lett. 294:109-112(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO OTHER FAMILY 31 MEMBERS.

Entry informationi

Entry nameiAMYG_SCHOC
AccessioniPrimary (citable) accession number: P22861
Secondary accession number(s): Q92336
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 16, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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