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P22861

- AMYG_SCHOC

UniProt

P22861 - AMYG_SCHOC

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Protein

Glucoamylase 1

Gene

GAM1

Organism
Schwanniomyces occidentalis (Yeast) (Debaryomyces occidentalis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This glucoamylase has a specificity toward both alpha-1,4 and alpha-1,6 linkages.

Catalytic activityi

Hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.PROSITE-ProRule annotation
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei470 – 4701By similarity
Active sitei473 – 4731By similarity
Active sitei638 – 6381Proton donorBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. glucan 1,4-alpha-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH31. Glycoside Hydrolase Family 31.
mycoCLAPiGLA31A_DEBOC.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase 1 (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:GAM1
OrganismiSchwanniomyces occidentalis (Yeast) (Debaryomyces occidentalis)
Taxonomic identifieri27300 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeSchwanniomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 958936Glucoamylase 1PRO_0000018586Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi416 – 4161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi580 – 5801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi602 – 6021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi813 – 8131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi907 – 9071N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi26 – 4116Ser-richAdd
BLAST
Compositional biasi530 – 54213Ser/Thr-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 31 family.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22861-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIFLKLIKSI VIGLGLVSAI QAAPASSIGS SASASSSSES SQATIPNDVT
60 70 80 90 100
LGVKQIPNIF NDSAVDANAA AKGYDLVNVT NTPRGLTGIL KLKEATNIYG
110 120 130 140 150
YDFDYLNLTV EYQADTRLNV HIEPTDLSDV FVLPEHLVVK PLVEGDAQSY
160 170 180 190 200
NFDNSDLVFE YSNTDFSFEV IRSSTKEVLF STKGNPLVFS NQFIQFNSSL
210 220 230 240 250
PKNHVITGLG ESIHGLVNEP GSVKTLFAND VGDPIDGNIY GVHPVYLDQR
260 270 280 290 300
YDTETTHAVY WRTSAIQEVL IGEESITWRA LSGVIDLYFF SGPTPKDAIQ
310 320 330 340 350
QYVKEIGLPA FQPYWSLGYH QCRWGYDTIE KLSEVVENFK KFNIPLETIW
360 370 380 390 400
SDIDYMDSYK DFTYDPHRFP LDEYRKFLDE LHKNNQHYVP ILDAAIYVPN
410 420 430 440 450
PNNATDNEYQ PFHYGNETDV FLKNPDGSLY IGAVWQVTLF SRFLSRKHSD
460 470 480 490 500
MDKVIKDWYE LTPFDGIWAD MNEVSSFCVG SCGTGKYFEN PAYPPFTVGS
510 520 530 540 550
KATSYPVGFD VSNASEWKSI QSSISATAKT SSTSSVSSSS STIDYMNTLA
560 570 580 590 600
PGKGNINYPP YAIYNMQGDS DLATHAVSPN ATHADGTVEY DIHNLYGYLQ
610 620 630 640 650
ENATYHALLE VFPNKRPFMI SRSTFPRAGK WTGHWGGDNT ADWAYAYFSI
660 670 680 690 700
PQAFSMGIAG LPFFGADVCG FNGNSDSELC SRWMQLGSFF PFYRNHNYLG
710 720 730 740 750
AIDQEPYVWE SVAEATRTSM AIRYLLLPYY YTLLHESHTT GLPILRAFSW
760 770 780 790 800
QFPNDRSLSG VDNQFFVGDG LVVTPVLEPG VDKVKGVFPG AGKEEVYYDW
810 820 830 840 850
YTQREVHFKD GKNETLDAPL GHIPLHIRGG NVLPTQEPGY TVAESRQNPF
860 870 880 890 900
GLIVALDNDG KAQGSLYLDD GESLVVDSSL LVSFSVSDNT LSASPSGDYK
910 920 930 940 950
ADQPLANVTI LGVGHKPKSV KFENANVDFT YKKSTVFVTG LDKYTKDGAF

SKDFTITW
Length:958
Mass (Da):106,508
Last modified:August 1, 1991 - v1
Checksum:i47938DB9BC308260
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60207 Genomic DNA. Translation: AAA33923.1.
PIRiJN0102.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60207 Genomic DNA. Translation: AAA33923.1 .
PIRi JN0102.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH31. Glycoside Hydrolase Family 31.
mycoCLAPi GLA31A_DEBOC.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF01055. Glyco_hydro_31. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 2 hits.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of the Schwanniomyces occidentalis glucoamylase gene (GAM1) and its expression in Saccharomyces cerevisiae."
    Dohmen R.J., Strasser A.W.M., Dahlems U.M., Hollenberg C.P.
    Gene 95:111-121(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 26076 / DSM 3794 / CBS 2864 / BCRC 22059 / NCYC 954 / NRRL Y-2470.
  2. "Striking structural and functional similarities suggest that intestinal sucrase-isomaltase, human lysosomal alpha-glucosidase and Schwanniomyces occidentalis glucoamylase are derived from a common ancestral gene."
    Naim H.Y., Niermann T., Kleinhans U., Hollenberg C.P., Strasser A.W.M.
    FEBS Lett. 294:109-112(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SIMILARITY TO OTHER FAMILY 31 MEMBERS.

Entry informationi

Entry nameiAMYG_SCHOC
AccessioniPrimary (citable) accession number: P22861
Secondary accession number(s): Q92336
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 1, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3