Reviewed, UniProtKB/Swiss-Prot P22859 (RBL1B_CHRVI)

Names and origin

Protein names

Recommended name:
    Ribulose bisphosphate carboxylase large chain 2
      Short name=RuBisCO large subunit 2
    EC=4.1.1.39

Gene names

Name:	cbbL2
Synonyms:	rbcL

Organism

Chromatium vinosum (Allochromatium vinosum)

Taxonomic identifier

1049 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Allochromatium

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site. HAMAP MF_01338
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP MF_01338 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP MF_01338
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers By similarity.
Post-translational modification	The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover By similarity.
Miscellaneous	The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity.
Sequence similarities	Belongs to the RuBisCO large chain family. Type I subfamily.
Caution	In C.vinosum two similar set of genes code for RuBisCO large and small chains: the rbcL-rbcS and the rbcA-rbcB sets. Under standard photoautotrophic culture conditions only the latter set seems active, the former probably being cryptic.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation Photosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Lyase Monooxygenase Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 471	470	Ribulose bisphosphate carboxylase large chain 2 HAMAP MF_01338		PRO_0000062622
Sites
	Active site	168	1	Proton acceptor By similarity
	Active site	287	1	Proton acceptor By similarity
	Metal binding	194	1	Magnesium; via carbamate group By similarity
	Metal binding	196	1	Magnesium By similarity
	Metal binding	197	1	Magnesium By similarity
	Binding site	116	1	Substrate; in homodimeric partner By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	170	1	Substrate By similarity
	Binding site	288	1	Substrate By similarity
	Binding site	320	1	Substrate By similarity
	Binding site	372	1	Substrate By similarity
	Site	327	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	194	1	N6-carboxylysine By similarity
	Disulfide bond	240		Interchain; in linked form By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22859-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FB79F09C6C33B885	FASTA	471	52,394
	10 20 30 40 50 60 MSTKTYDAGV KDYALTYWTP DYVPLDSDLL ACFKVTPQAK VSREEAAAAV AAESSTGTWT 70 80 90 100 110 120 TVWSDLLTDL DYYKGRAYRI EDVPGDKESF YAFIAYPLDL FEEGSIVNVL TSLVGNVFGF 130 140 150 160 170 180 KAVRALRLED IRFPLHYVKT CGGPRNGIQV ERDRMDKYGR PFLGATVKPK LGLSAKNYGR 190 200 210 220 230 240 AVYEMLRGGL DFTKDDENVN SQPFMRWQNR FEFVSEAVRK AREETGERKG HYLNVTAPTC 250 260 270 280 290 300 EEMFKRAEFA KECGAPIIMH DFLTGGFTAN TSLANWCRDN GMLLHIHRAM HAVIDRHPKH 310 320 330 340 350 360 GIHFRVLAKC LRLSGGDHLH TGTVVGKLEG DRQSTLGFVD QLRESFIPED RSRGLFFDQD 370 380 390 400 410 420 WGGMPGVMAV ASGGIHVWHI PALVTIFGDD SVLQFGGGTQ GHPWGNAAGA AANRVATEAC 430 440 450 460 470 VKARNEGVEI EKHAREVLSD AARHSPELAV AMETWKEIKF EFDVVDKLDA A

References

[1]	"Sequence and expression of genes encoding the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase from Chromatium vinosum." Kobayashi H., Viale A.M., Takabe T., Akazawa T., Wada K., Shinozaki K., Kobayashi K., Sugiura M. Gene 97:55-62(1991) [PubMed: 1899846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17899 / DSM 180 / D.
[2]	"Distinct properties of Escherichia coli products of plant-type ribulose-1,5-bisphosphate carboxylase/oxygenase directed by two sets of genes from the photosynthetic bacterium Chromatium vinosum." Viale A.M., Kobayashi H., Akazawa T. J. Biol. Chem. 265:18386-18392(1990) [PubMed: 2211708] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D90204 Genomic DNA. Translation: BAA14229.1.
PIR	RKKRL2. JQ0586.
3D structure databases
HSSP	HSSP built from PDB template 1GEH based on UniProtKB O93627.
SMR	P22859. Positions 5-467, 6-468.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.1.1.39. 2799.
Family and domain databases
HAMAP	MF_01338. [Tree]
InterPro	IPR000685. RuBisCO_lsu_C. IPR017443. RuBisCO_lsu_fd_N. IPR017444. RuBisCO_lsu_N. [Graphical view]
Gene3D	G3DSA:3.20.20.110. RuBisCO_large. 1 hit. G3DSA:3.30.70.150. RuBisCO_large. 1 hit.
Pfam	PF00016. RuBisCO_large. 1 hit. PF02788. RuBisCO_large_N. 1 hit. [Graphical view]
PROSITE	PS00157. RUBISCO_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RBL1B_CHRVI

Accession

Primary (citable) accession number: P22859

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	January 23, 2007
Last modified:	March 24, 2009
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)