P22856 (VL96_IRV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative ubiquitin thioesterase L96
EC=3.4.19.12

Gene names

ORF Names:

L96

Organism

Tipula iridescent virus (TIV) (Insect iridescent virus type 1)

Taxonomic identifier

10490 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Iridoviridae › Iridovirus › Invertebrate iridescent virus 1

Virus host

Tipula [TaxID: 41043]

Protein attributes

Sequence length	867 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation By similarity. May be involved in TIV genomic DNA packaging in a manner related to the Gag polyproteins of the mammalian viruses. Ref.1
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway Viral genome packaging
Domain	Repeat
Ligand	DNA-binding
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 867	867	Putative ubiquitin thioesterase L96		PRO_0000222389
Regions
	Domain	606 – 745	140	OTU
Sites
	Active site	614	1	Potential
	Active site	617	1	Nucleophile By similarity
	Active site	738	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P22856 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: F19DBDE8FE5CA103	FASTA	867	96,012
	10 20 30 40 50 60 MCDIDLKTCE KSSKYKKKDI VDLGKKCGVD PYLPNGREKT RQVICTEIVN QYSTNPPSSS 70 80 90 100 110 120 SESDDDEDMG KQDQDPICGI SEKECNKLDN VNLLALGEYC GVDIYTPEGN LKTSKTICKS 130 140 150 160 170 180 VSLKQSSPKA QKSPNKNDLI NMDPANLKKL AKKLDIDWKK KDIKELQYLI AKKLNSIKSV 190 200 210 220 230 240 SPSRSPSPRR SRSPSPNIQN MKKKELKALA KALGASDKKL DKMDKNQLIE YILSRKKSPS 250 260 270 280 290 300 PVRKSRSPSL RQRSRSPGRS RSNSPVRPKY RAADLLGKKV VELKELAKME GLKKWKDKTP 310 320 330 340 350 360 SKMLKQDLVD FLLHVGGGGQ LPKTPSPVKP SASPKTRAEL ALLKVPELKA MALSYGLKNF 370 380 390 400 410 420 KDKTPSQLRK NDFIDFIILS TQKSQSPPPS IRSKPRSSSP KYKTKDLVTT DESDGEIVVK 430 440 450 460 470 480 KITRKPKSPR RSPPASVRRS RTPSVPKSPS ARPRSKSPSV RAEITDDEGE TPPSSVRPKS 490 500 510 520 530 540 PSVRPKSPSV RPRSKSPSVR PKSPSARPRS KSPSVRSKSP SVRPKSPSVR PKSPSVRPRS 550 560 570 580 590 600 KSPSVRPKSP SVRPPPPDPS NSPSITVDPS VTPPSSVKIK KRPELPEYKG GNADRDLEIL 610 620 630 640 650 660 ARRRGYKVIP VKGDGNCLFR AVGKSLRLNQ NIKYSHEDLR AQVVTYLTSH KEFLEPYLEY 670 680 690 700 710 720 VTESGDTTPQ EYAKNVERYI KNISKPGTWG DFICLRVLSE ILKVKFNLLI LNTRNFQVIS 730 740 750 760 770 780 NNDTFKPLIP LGFIDDYHYT ALTPLYAEPI AVLENETPTP SIAPSIRPPP SIIPSIAPSV 790 800 810 820 830 840 RPSIAPSVRP SIVPATPSIV PSLKPSVVPK LTPSIAPKIP PPVFGPVKPL SSTRELLEEA 850 860 DKVHPYVRED ISQLARAEEQ ILVSLGM

References

[1]	"Molecular cloning and characterization of a late Tipula iridescent virus gene." Home W.A., Tajbakhsh S., Seligy V.L. Gene 94:243-248(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62953 Genomic DNA. Translation: AAA47919.1.
PIR	JH0225.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR003323. OTU. [Graphical view]
Pfam	PF02338. OTU. 1 hit. [Graphical view]
PROSITE	PS50802. OTU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VL96_IRV1

Accession

Primary (citable) accession number: P22856

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	April 3, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program