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Protein

Alpha-mannosidase

Gene

AMS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Degrades free oligosaccharides in the vacuole.1 Publication

Miscellaneous

It is not specific for the 1,2-alpha-mannosidic linkage.
Present with 319 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi298ZincBy similarity1
Metal bindingi300ZincBy similarity1
Active sitei411NucleophileBy similarity1
Metal bindingi411ZincBy similarity1
Metal bindingi626ZincBy similarity1

GO - Molecular functioni

  • alpha-mannosidase activity Source: SGD
  • carbohydrate binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular carbohydrate metabolic process Source: SGD
  • mannose catabolic process Source: SGD
  • mannose metabolic process Source: GO_Central
  • oligosaccharide catabolic process Source: SGD
  • protein deglycosylation Source: GO_Central

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YGL156W-MONOMER
YEAST:YGL156W-MONOMER
ReactomeiR-SCE-8853383 Lysosomal oligosaccharide catabolism

Protein family/group databases

CAZyiGH38 Glycoside Hydrolase Family 38

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase (EC:3.2.1.24)
Alternative name(s):
Alpha-D-mannoside mannohydrolase
Gene namesi
Name:AMS1
Ordered Locus Names:YGL156W
ORF Names:G1861
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL156W
SGDiS000003124 AMS1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002069102 – 1083Alpha-mannosidaseAdd BLAST1082

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP22855
PaxDbiP22855
PRIDEiP22855

PTM databases

iPTMnetiP22855

Interactioni

Subunit structurei

Composed of isoforms with three constituent polypeptides described as [(107 kDa)-n (73 kDa)-(6-n) (31 kDa)-(6-n)], where n is 0-6. The 73 kDa and the 31 kDa polypeptides may be proteolytic derivatives of the 107 kDa polypeptide in the vacuole. Oligomerizes in the cytoplasm and retains its oligomeric form during import into the vacuole.

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG34Q122923EBI-10398,EBI-36362

Protein-protein interaction databases

BioGridi33098, 45 interactors
DIPiDIP-5497N
IntActiP22855, 7 interactors
MINTiP22855
STRINGi4932.YGL156W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JM0electron microscopy6.30A1-1070[»]
ProteinModelPortaliP22855
SMRiP22855
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000006338
HOGENOMiHOG000048425
InParanoidiP22855
KOiK01191
OMAiGQYWDAW
OrthoDBiEOG092C07TR

Family and domain databases

Gene3Di1.20.1270.50, 1 hit
3.20.110.10, 1 hit
InterProiView protein in InterPro
IPR011013 Gal_mutarotase_sf_dom
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR011682 Glyco_hydro_38_C
IPR015341 Glyco_hydro_38_cen
IPR037094 Glyco_hydro_38_cen_sf
IPR000602 Glyco_hydro_38_N
IPR027291 Glyco_hydro_38_N_sf
IPR028995 Glyco_hydro_57/38_cen_sf
PfamiView protein in Pfam
PF09261 Alpha-mann_mid, 1 hit
PF01074 Glyco_hydro_38, 1 hit
PF07748 Glyco_hydro_38C, 1 hit
SMARTiView protein in SMART
SM00872 Alpha-mann_mid, 1 hit
SUPFAMiSSF74650 SSF74650, 1 hit
SSF88688 SSF88688, 1 hit
SSF88713 SSF88713, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEDIIYDP QFKPVQGIYE NRLRQFIDTG GDYHDLNLPK FYDKKRISLD
60 70 80 90 100
HDHVKVWWYQ VSFERGSSPV SPDKRPSWKS IIERDKKGEL EFREANINQP
110 120 130 140 150
FGPSWSTTWF KVKISLPEDW VKSNEQLLFQ WDCSNEGIVI DPKTLIPVTA
160 170 180 190 200
FSGGERTEYV LPKTSDGKHF FYIEAGNNGM FGCGAGSTIN PPDDNRFFHL
210 220 230 240 250
RKADIVWPDL DARALYIDFW MLGDAARELP GDSWQKHQAR QLGNAVMNLF
260 270 280 290 300
DPNDRSSVRK CRELLQREYF DSFLESSKVY EQGESQVLTN VYGIGNCHID
310 320 330 340 350
TAWLWPFAET RRKIVRSWSS QCTLMDRFPE YKFVASQAQQ FKWLLEDHPE
360 370 380 390 400
FFNKVLIPKI QQSQFFAVGG TWVENDTNIP SGESLARQFF FGQRFFLKHF
410 420 430 440 450
GLKSKIFWLP DTFGYSSQMP QLCRLSGIDK FLTQKLSWNN INSFPHSTFN
460 470 480 490 500
WAGIDGSQLL THMPPGNTYT ADSHFGDVLR TAKQNKTPEY YGSGLMLYGK
510 520 530 540 550
GDGGGGPTEE MLQKMRRIRS MNNRNGNVIP KLQVGITVDE FYDDILKRTN
560 570 580 590 600
QGHDLPTWSG ELYFEFHRGT YTSQAQTKKL MRLSEIKLHD LEWIAAKTSV
610 620 630 640 650
LYPDSYKYPS KQINELWENV LLCQFHDVLP GSCIEMVYKY EAVPMLHNVV
660 670 680 690 700
KECTSLIDKT VQFLQSQSKA DLVEMRTLTW SKPEKVSEEC SLNGSYTSSV
710 720 730 740 750
TGYDDYIVLA NGKLKVIICK KTGVITSITD ETLGVEYLDT EHGRNKLGAN
760 770 780 790 800
QFVIYDDKPL GWQAWDTELY SVNQYKYVTK PKKVQVSCNT KEKCAVEVIF
810 820 830 840 850
QISEKCKIKS VISLNATAVT DAKLSKVDIS TTVENWDARN KFLKVEFPVN
860 870 880 890 900
IRNDFASYET QFGITKRPTH YNTSWDVAKF EVCHHKFADY SEYSKGVSIL
910 920 930 940 950
NDCKYGFSTH GNLMRLSLLR SPKAPDAHAD MGTHEIKYAI YPHRGALSSD
960 970 980 990 1000
TVKLAHEFNY CFKYKLPKDI GMNFDDIISI SGDENVILSN IKRGEDDSAV
1010 1020 1030 1040 1050
KSNYSLNPRD EQSIVVRVYE SLGGESFASL NTTLNLKRIE KVDNLEMKVY
1060 1070 1080
KSLTATRDES NHAINRIPIK LRPFEIASFR LYF
Length:1,083
Mass (Da):124,499
Last modified:February 1, 1996 - v2
Checksum:i624C818040FF5127
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti786V → L in AAA34423 (PubMed:2675832).Curated1
Sequence conflicti798V → A in AAA34423 (PubMed:2675832).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29146 Genomic DNA Translation: AAA34423.1
Z48618 Genomic DNA Translation: CAA88536.1
Z72678 Genomic DNA Translation: CAA96868.1
BK006941 Genomic DNA Translation: DAA07956.1
PIRiS60420 S53048
RefSeqiNP_011359.1, NM_001181021.1

Genome annotation databases

EnsemblFungiiYGL156W; YGL156W; YGL156W
GeneIDi852721
KEGGisce:YGL156W

Similar proteinsi

Entry informationi

Entry nameiMAN1_YEAST
AccessioniPrimary (citable) accession number: P22855
Secondary accession number(s): D6VTZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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