Reviewed, UniProtKB/Swiss-Prot P22851 (RIPB_LUFCY)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein luffin-B     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Luffa cylindrica (Smooth loofah) (Sponge gourd)
Taxonomic identifier	3670 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Luffa

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Antiviral defense Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 250

250

Ribosome-inactivating protein luffin-B

PRO_0000221416

Sites

Active site

160

1

By similarity

Secondary structure

1

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250

Helix Strand Turn

Details...

Beta strand

3 – 6

4

Helix

12 – 25

14

Beta strand

28 – 32

5

Beta strand

35 – 38

4

Helix

44 – 47

4

Beta strand

48 – 54

7

Beta strand

60 – 66

7

Turn

67 – 69

3

Beta strand

72 – 77

6

Beta strand

80 – 83

4

Helix

87 – 92

6

Turn

93 – 95

3

Beta strand

100 – 104

5

Helix

111 – 118

8

Helix

122 – 124

3

Helix

129 – 140

12

Turn

144 – 146

3

Helix

147 – 157

11

Helix

159 – 163

5

Helix

165 – 173

9

Beta strand

175 – 177

3

Helix

183 – 203

21

Turn

204 – 208

5

Beta strand

209 – 217

9

Beta strand

223 – 228

6

Helix

232 – 235

4

Turn

236 – 239

4

Helix

246 – 248

3

Sequences

	Sequence		Length	Mass (Da)
	P22851-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: F01A8DC8A1078700	FASTA	250	27,293
	10 20 30 40 50 60 ANVSFSLSGA DSKSYSKFIT ALRKALPSKE KVSNIPLLLP SASGASRYIL MQLSNYDAKA 70 80 90 100 110 120 ITMAIDVTNV YIMGYLVNST SYFANESDAK LASQYVFKGS TLVTIPYSGN YERLQNAAGK 130 140 150 160 170 180 IREKIPLGFR ALDSALTSIF HYDSTAAAAA FLVILQTTAE ASRFKYIEGQ IIERIPKNEV 190 200 210 220 230 240 PSPAALSLEN EAWSLLSKQI QLAQTNNGAF RTPVVIIDNK GQRVEITNLA SKVQIKDVNS 250 KLLLNKQNIA

References

[1]	"Complete amino acid sequence of luffin-b, a ribosome-inactivating protein from sponge gourd (Luffa cylindrica) seeds." Islam M.R., Hirayama H., Funatsu G. Agric. Biol. Chem. 55:229-238(1991) [PubMed: 1368666] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Seed.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

JN0108.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NIO	X-ray	2.00	A	1-250	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.2.2.22. 273560.

Family and domain databases

InterPro

IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]

Gene3D

G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.

Pfam

PF00161. RIP. 1 hit.
[Graphical view]

PRINTS

PR00396. SHIGARICIN.

PROSITE

PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RIPB_LUFCY

Accession

Primary (citable) accession number: P22851

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)