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Reviewed, UniProtKB/Swiss-Prot P22849 (RBL1A_CHRVI)

Last modified March 24, 2009. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Ribulose bisphosphate carboxylase large chain 1
      Short name=RuBisCO large subunit 1
    EC=4.1.1.39
Gene names
Name: cbbL1
Synonyms: rbcA
OrganismChromatium vinosum (Allochromatium vinosum)
Taxonomic identifier1049 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Chromatiaceae › Allochromatium

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Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site. HAMAP MF_01338

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP MF_01338

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP MF_01338

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Heterohexadecamer of 8 large chains and 8 small chains By similarity.

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity.

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily.

Caution

In C.vinosum two similar set of genes code for RuBisCO large and small chains: the rbcL-rbcS and the rbcA-rbcB sets. Under standard photoautotrophic culture conditions only the latter set seems active, the former probably being cryptic.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2 Ref.3 Ref.4
Chain2 – 472471Ribulose bisphosphate carboxylase large chain 1 HAMAP MF_01338
PRO_0000062621

Sites

Active site1671Proton acceptor By similarity
Active site2861Proton acceptor By similarity
Metal binding1931Magnesium; via carbamate group By similarity
Metal binding1951Magnesium By similarity
Metal binding1961Magnesium By similarity
Binding site1151Substrate; in homodimeric partner By similarity
Binding site1651Substrate By similarity
Binding site1691Substrate By similarity
Binding site2871Substrate By similarity
Binding site3191Substrate By similarity
Binding site3711Substrate By similarity
Site3261Transition state stabilizer By similarity

Amino acid modifications

Modified residue1931N6-carboxylysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
P22849-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 2512B82C3A8D1895

FASTA47252,524
        10         20         30         40         50         60 
MAKTYSAGVK EYRETYWMPN YTPKDTDILA CFKITPQAGV PREEAAAAVA AESSTGTWTT 

        70         80         90        100        110        120 
VWTDLLTDLD YYKGRAYAIE DVPGDDTCFY AFIAYPIDLF EEGSVVNVFT SLVGNVFGFK 

       130        140        150        160        170        180 
AVRALRLEDV RFPIAYVMTC NGPPHAIQVE RDIMNKYGRP MLGCTIKPKL GLSAKNYGRA 

       190        200        210        220        230        240 
VYECLRGGLD FTKDDENVNS QPFMRWRQRF DFVMDAIDKA ERETGERKGH YLNVTAPTPE 

       250        260        270        280        290        300 
EMYKRAEYAK EIGAPIIMHD YITGGFCANT GLAQWCRDNG VLLHIHRAMH AVLDRNPHHG 

       310        320        330        340        350        360 
IHFRVLTKIL RLSGGDHLHT GTVVGKLEGD RASTLGWIDL LRESYIKEDR SRGLFFDQDW 

       370        380        390        400        410        420 
GSMPGAFAVA SGGIHVWHMP ALVTIFGDDS VLQFGGGTLG HPWGNAAGAC ANRVALEACV 

       430        440        450        460        470 
EARNQGVAIE KEGKDVLTKA AASSPELKIA METWKEIKFE FDTVDKLDIA HK

« Hide

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References

[1]"Expressed genes for plant-type ribulose 1,5-bisphosphate carboxylase/oxygenase in the photosynthetic bacterium Chromatium vinosum, which possesses two complete sets of the genes."
Viale A.M., Kobayashi H., Akazawa T.
J. Bacteriol. 171:2391-2400(1989) [PubMed: 2708310] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Distinct properties of Escherichia coli products of plant-type ribulose-1,5-bisphosphate carboxylase/oxygenase directed by two sets of genes from the photosynthetic bacterium Chromatium vinosum."
Viale A.M., Kobayashi H., Akazawa T.
J. Biol. Chem. 265:18386-18392(1990) [PubMed: 2211708] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-25.
[3]"The nature of L8 and L8S8 forms of ribulose bisphosphate carboxylase/oxygenase from Chromatium vinosum."
Torres-Ruiz J., McFadden B.A.
Arch. Biochem. Biophys. 254:63-68(1987) [PubMed: 3579306] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-21.
[4]"Sequence and expression of genes encoding the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase from Chromatium vinosum."
Kobayashi H., Viale A.M., Takabe T., Akazawa T., Wada K., Shinozaki K., Kobayashi K., Sugiura M.
Gene 97:55-62(1991) [PubMed: 1899846] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-12.

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Cross-references

Sequence databases

M26396 Genomic DNA. Translation: AAA23328.1.
PIRRKKRL1. A32303.

3D structure databases

HSSPHSSP built from PDB template 1GEH based on UniProtKB O93627.
SMRP22849. Positions 5-467.
ModBaseSearch...

Enzyme and pathway databases

BRENDA4.1.1.39. 2799.

Family and domain databases

HAMAPMF_01338.
[Tree]
InterProIPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
Gene3DG3DSA:3.20.20.110. RuBisCO_large. 1 hit.
G3DSA:3.30.70.150. RuBisCO_large. 1 hit.
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
PROSITEPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameRBL1A_CHRVI
AccessionPrimary (citable) accession number: P22849
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: March 24, 2009
This is version 64 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents