P22842 (XYLA_THEP3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xylose isomerase
EC=5.3.1.5

Gene names

Name:	xylA
Ordered Locus Names:	Teth39_2060

Organism

Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) (Clostridium thermohydrosulfuricum) [Complete proteome] [HAMAP]

Taxonomic identifier

340099 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteraceae › Thermoanaerobacter › Thermoanaerobacter pseudethanolicus

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-xylose = D-xylulose. HAMAP-Rule MF_00455
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm HAMAP-Rule MF_00455.
Sequence similarities	Belongs to the xylose isomerase family.
Sequence caution	The sequence AAC46145.1 differs from that shown. Reason: Erroneous initiation. The sequence ABY95684.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Pentose shunt Xylose metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	D-xylose metabolic process Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP xylose isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Xylose isomerase HAMAP-Rule MF_00455		PRO_0000195812
Sites
	Active site	100	1	By similarity
	Active site	103	1	By similarity
	Metal binding	231	1	Magnesium 1 By similarity
	Metal binding	267	1	Magnesium 1 By similarity
	Metal binding	267	1	Magnesium 2 By similarity
	Metal binding	270	1	Magnesium 2 By similarity
	Metal binding	295	1	Magnesium 1 By similarity
	Metal binding	306	1	Magnesium 2 By similarity
	Metal binding	308	1	Magnesium 2 By similarity
	Metal binding	338	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22842 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 4B3B0BC1B7413DE7	FASTA	438	50,158
	10 20 30 40 50 60 MEYFKNVPQI KYEGPKSNNP YAFKFYNPDE IIDGKPLKEH LRFSVAYWHT FTANGTDPFG 70 80 90 100 110 120 APTMQRPWDH FTDPMDIAKA RVEAAFELFE KLDVPFFCFH DRDIAPEGET LRETNKNLDT 130 140 150 160 170 180 IVAMIKDYLK TSKTKVLWGT ANLFSNPRFV HGAATSCNAD VFAYAAAQVK KALEITKELG 190 200 210 220 230 240 GQNYVFWGGR EGYETLLNTD MELELDNLAR FLHMAVEYAQ EIGFEGQFLI EPKPKEPTKH 250 260 270 280 290 300 QYDFDAASVH AFLKKYDLDK YFKLNIEANH ATLAGHDFQH ELRYARINNM LGSIDANMGD 310 320 330 340 350 360 MLLGWDTDQY PTDIRMTTLA MYEVIKMGGF NKGGLNFDAK VRRASFEPED LFLGHIAGMD 370 380 390 400 410 420 AFAKGFKVAY KLVKDGVFDR FIEERYKSYR EGIGAEIVSG KANFKTLEEY ALNNPKIENK 430 SGKQELLESI LNQYLFSE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Xylose (glucose) isomerase gene from the thermophile Clostridium thermohydrosulfuricum; cloning, sequencing, and expression in Escherichia coli." Dekker K., Yamagata H., Sakaguchi K., Udaka S. Agric. Biol. Chem. 55:221-227(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and characterization of transcription of the xylAB operon in Thermoanaerobacter ethanolicus." Erbeznik M., Dawson K.A., Strobel H.J. J. Bacteriol. 180:1103-1109(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequence of Thermoanaerobacter pseudethanolicus 39E." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Bruce D., Goodwin L., Saunders E., Brettin T., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Hemme C., Fields M.W., He Z., Zhou J., Richardson P. Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33223 / 39E.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00756 Genomic DNA. Translation: BAA00652.1. AF001974 Genomic DNA. Translation: AAC46145.1. Different initiation. CP000924 Genomic DNA. Translation: ABY95684.1. Different initiation.
RefSeq	YP_001666020.1. NC_010321.1.
3D structure databases
ProteinModelPortal	P22842.
SMR	P22842. Positions 2-437.
ModBase	Search...
Protein-protein interaction databases
STRING	340099.Teth39_2060.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABY95684; ABY95684; Teth39_2060.
GeneID	5875035.
KEGG	tpd:Teth39_2060.
PATRIC	23888937. VBIThePse6203_2157.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2115.
HOGENOM	HOG000252293.
KO	K01805.
OMA	HTFQHEL.
ProtClustDB	CLSK986031.
Family and domain databases
Gene3D	3.20.20.150. 1 hit.
HAMAP	MF_00455. Xylose_isom_A.
InterPro	IPR013022. Xyl_isomerase-like_TIM-brl. IPR013452. Xylose_isom_bac. IPR001998. Xylose_isomerase. [Graphical view]
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
PRINTS	PR00688. XYLOSISMRASE.
SUPFAM	SSF51658. Xyl_isomerase-like_TIM-brl. 1 hit.
TIGRFAMs	TIGR02630. xylose_isom_A. 1 hit.
PROSITE	PS51415. XYLOSE_ISOMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLA_THEP3

Accession

Primary (citable) accession number: P22842
Secondary accession number(s): B0K7B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program