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Protein

Nuclear receptor subfamily 4 group A member 1

Gene

Nr4a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi263 – 338Nuclear receptorPROSITE-ProRule annotationAdd BLAST76
Zinc fingeri266 – 286NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri302 – 326NR C4-typePROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

  • glucocorticoid receptor binding Source: UniProtKB
  • nuclear receptor activity Source: InterPro
  • protein heterodimerization activity Source: UniProtKB
  • sequence-specific DNA binding Source: InterPro
  • steroid hormone receptor activity Source: InterPro
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: UniProtKB
  • transcription factor binding Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Receptor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-RNO-198693 AKT phosphorylates targets in the nucleus
R-RNO-383280 Nuclear Receptor transcription pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 4 group A member 1
Alternative name(s):
NUR77
Nerve growth factor-induced protein I-B
Short name:
NGFI-B
Orphan nuclear receptor HMR
Gene namesi
Name:Nr4a1
Synonyms:Hmr, Ngfib
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi620029 Nr4a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi340S → A: Loss of phosphorylation. 1 Publication1
Mutagenesisi345R → K: Decreased NBRE binding. 1 Publication1
Mutagenesisi348L → V: Almost complete loss of NBRE binding. 1 Publication1
Mutagenesisi350S → A: Loss of phosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000537171 – 597Nuclear receptor subfamily 4 group A member 1Add BLAST597

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei340Phosphoserine; by PKA1 Publication1
Modified residuei350Phosphoserine; by PKA, RPS6KA1 and RPS6KA31 Publication1

Post-translational modificationi

Phosphorylated at Ser-350 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli (By similarity). Phosphorylation of Ser-350 results in decrease in NBRE binding while phosphorylation of Ser-340 has little effect on it.By similarity1 Publication
Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP22829
PRIDEiP22829

PTM databases

iPTMnetiP22829
PhosphoSitePlusiP22829

Expressioni

Tissue specificityi

Expressed in lung, brain and superior cervical ganglia. High levels are seen in the adrenal tissue.

Inductioni

By nerve growth factor and during liver regeneration.

Gene expression databases

BgeeiENSRNOG00000007607
ExpressionAtlasiP22829 baseline and differential
GenevisibleiP22829 RN

Interactioni

Subunit structurei

Interacts with GADD45GIP1. Interacts with STK11 (By similarity). Binds DNA as a monomer.By similarity1 Publication

GO - Molecular functioni

  • glucocorticoid receptor binding Source: UniProtKB
  • protein heterodimerization activity Source: UniProtKB
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

IntActiP22829, 2 interactors
MINTiP22829
STRINGi10116.ENSRNOP00000010171

Structurei

Secondary structure

1597
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi267 – 269Combined sources3
Beta strandi275 – 277Combined sources3
Beta strandi280 – 282Combined sources3
Helixi284 – 296Combined sources13
Beta strandi303 – 306Combined sources4
Turni312 – 317Combined sources6
Helixi319 – 328Combined sources10
Helixi333 – 335Combined sources3
Helixi339 – 341Combined sources3
Helixi363 – 374Combined sources12
Helixi378 – 380Combined sources3
Helixi399 – 421Combined sources23
Turni424 – 428Combined sources5
Helixi431 – 453Combined sources23
Turni456 – 459Combined sources4
Beta strandi460 – 462Combined sources3
Beta strandi466 – 470Combined sources5
Helixi471 – 478Combined sources8
Helixi481 – 495Combined sources15
Helixi499 – 510Combined sources12
Helixi520 – 540Combined sources21
Helixi550 – 555Combined sources6
Helixi558 – 578Combined sources21
Helixi585 – 592Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CITX-ray2.70A264-352[»]
1YJEX-ray2.40A354-597[»]
ProteinModelPortaliP22829
SMRiP22829
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22829

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini359 – 594NR LBDPROSITE-ProRule annotationAdd BLAST236

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi80 – 91Poly-SerAdd BLAST12
Compositional biasi182 – 186Poly-Pro5
Compositional biasi582 – 585Poly-Pro4

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri266 – 286NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri302 – 326NR C4-typePROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG4217 Eukaryota
ENOG410YWNC LUCA
GeneTreeiENSGT00760000118887
HOGENOMiHOG000230925
HOVERGENiHBG052663
InParanoidiP22829
KOiK04465
OMAiTDRHGLQ
OrthoDBiEOG091G0GUM
PhylomeDBiP22829
TreeFamiTF315430

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR035500 NHR_like_dom_sf
IPR003071 Nuc_orp_HMR_rcpt
IPR003070 Nuc_orph_rcpt
IPR000536 Nucl_hrmn_rcpt_lig-bd
IPR001723 Nuclear_hrmn_rcpt
IPR001628 Znf_hrmn_rcpt
IPR013088 Znf_NHR/GATA
PfamiView protein in Pfam
PF00104 Hormone_recep, 1 hit
PF00105 zf-C4, 1 hit
PRINTSiPR01285 HMRNUCRECPTR
PR01284 NUCLEARECPTR
PR00398 STRDHORMONER
PR00047 STROIDFINGER
SMARTiView protein in SMART
SM00430 HOLI, 1 hit
SM00399 ZnF_C4, 1 hit
SUPFAMiSSF48508 SSF48508, 1 hit
PROSITEiView protein in PROSITE
PS51843 NR_LBD, 1 hit
PS00031 NUCLEAR_REC_DBD_1, 1 hit
PS51030 NUCLEAR_REC_DBD_2, 1 hit

Sequencei

Sequence statusi: Complete.

P22829-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPCIQAQYGT PATSPGPRDH LTGDPLALEF SKPTMDLASP ETAPTAPATL
60 70 80 90 100
PSFSTFMDGG YTGEFDTFLY QLPGTAQPCS SASSTSSSSS SATSPASASF
110 120 130 140 150
KFEDFQVYGC YPGTLSGPLD ETLSSSGSDY YGSPCSAPSP PTPNFQPSQL
160 170 180 190 200
SPWDGSFGHF SPSQTYEGLR VWTEQLPKAS GPPPPPTFFS FSPPTGPSPS
210 220 230 240 250
LAQSSLKLFP APATHQLGEG ESYSVPAAFP GLAPTSPNCD TSGILDAPVT
260 270 280 290 300
STKARSGSSG GSEGRCAVCG DNASCQHYGV RTCEGCKGFF KRTVQKSAKY
310 320 330 340 350
ICLANKDCPV DKRRRNRCQF CRFQKCLAVG MVKEVVRTDS LKGRRGRLPS
360 370 380 390 400
KPKQPPDASP TNLLTSLIRA HLDSGPNTAK LDYSKFQELV LPRFGKEDAG
410 420 430 440 450
DVQQFYDLLS GSLDVIRKWA EKIPGFIELS PGDQDLLLES AFLELFILRL
460 470 480 490 500
AYRSKPGEGK LIFCSGLVLH RLQCARGFGD WIDNILAFSR SLHSLGVDVP
510 520 530 540 550
AFACLSALVL ITDRHGLQDP RRVEELQNRI ASCLKEHMAA VAGDPQPASC
560 570 580 590
LSRLLGKLPE LRTLCTQGLQ RIFCLKLEDL VPPPPIVDKI FMDTLSF
Length:597
Mass (Da):64,282
Last modified:October 1, 1996 - v2
Checksum:i9CFA987112337E53
GO

Sequence cautioni

The sequence AAA56770 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH97313 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17254 mRNA Translation: AAA56770.1 Different initiation.
BC097313 mRNA Translation: AAH97313.2 Different initiation.
PIRiJQ0623
RefSeqiNP_077364.2, NM_024388.2
XP_006242418.1, XM_006242356.2
XP_006242420.1, XM_006242358.2
XP_017450619.1, XM_017595130.1
UniGeneiRn.10000

Genome annotation databases

EnsembliENSRNOT00000010171; ENSRNOP00000010171; ENSRNOG00000007607
GeneIDi79240
KEGGirno:79240

Similar proteinsi

Entry informationi

Entry nameiNR4A1_RAT
AccessioniPrimary (citable) accession number: P22829
Secondary accession number(s): Q4V8M4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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