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P22829 (NR4A1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear receptor subfamily 4 group A member 1
Alternative name(s):
NUR77
Nerve growth factor-induced protein I-B
Short name=NGFI-B
Orphan nuclear receptor HMR
Gene names
Name:Nr4a1
Synonyms:Hmr, Ngfib
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length597 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation By similarity.

Subunit structure

Interacts with GADD45GIP1. Interacts with STK11 By similarity. Binds DNA as a monomer.

Subcellular location

Cytoplasm By similarity. Nucleus.

Tissue specificity

Expressed in lung, brain and superior cervical ganglia. High levels are seen in the adrenal tissue.

Induction

By nerve growth factor and during liver regeneration.

Post-translational modification

Phosphorylated at Ser-350 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli By similarity. Phosphorylation of Ser-350 results in decrease in NBRE binding while phosphorylation of Ser-340 has little effect on it. Ref.5

Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1 By similarity.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR4 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Sequence caution

The sequence AAA56770.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH97313.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionReceptor
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell migration involved in sprouting angiogenesis

Inferred from electronic annotation. Source: Ensembl

cellular response to fibroblast growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to vascular endothelial growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

endothelial cell chemotaxis

Inferred from electronic annotation. Source: Ensembl

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

skeletal muscle cell differentiation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay PubMed 15942958. Source: RGD

transcription factor complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from electronic annotation. Source: Ensembl

ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding

Inferred from electronic annotation. Source: Ensembl

steroid hormone receptor activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 597597Nuclear receptor subfamily 4 group A member 1
PRO_0000053717

Regions

DNA binding263 – 33876Nuclear receptor Ref.4
Zinc finger266 – 28621NR C4-type
Zinc finger302 – 32625NR C4-type
Region408 – 45851Ligand-binding Potential
Compositional bias80 – 9112Poly-Ser
Compositional bias182 – 1865Poly-Pro
Compositional bias582 – 5854Poly-Pro

Amino acid modifications

Modified residue3401Phosphoserine; by PKA Ref.5
Modified residue3501Phosphoserine; by PKA, RPS6KA1 and RPS6KA3 Ref.5

Experimental info

Mutagenesis3401S → A: Loss of phosphorylation.
Mutagenesis3451R → K: Decreased NBRE binding.
Mutagenesis3481L → V: Almost complete loss of NBRE binding.
Mutagenesis3501S → A: Loss of phosphorylation.

Secondary structure

............................................... 597
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22829 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 9CFA987112337E53

FASTA59764,282
        10         20         30         40         50         60 
MPCIQAQYGT PATSPGPRDH LTGDPLALEF SKPTMDLASP ETAPTAPATL PSFSTFMDGG 

        70         80         90        100        110        120 
YTGEFDTFLY QLPGTAQPCS SASSTSSSSS SATSPASASF KFEDFQVYGC YPGTLSGPLD 

       130        140        150        160        170        180 
ETLSSSGSDY YGSPCSAPSP PTPNFQPSQL SPWDGSFGHF SPSQTYEGLR VWTEQLPKAS 

       190        200        210        220        230        240 
GPPPPPTFFS FSPPTGPSPS LAQSSLKLFP APATHQLGEG ESYSVPAAFP GLAPTSPNCD 

       250        260        270        280        290        300 
TSGILDAPVT STKARSGSSG GSEGRCAVCG DNASCQHYGV RTCEGCKGFF KRTVQKSAKY 

       310        320        330        340        350        360 
ICLANKDCPV DKRRRNRCQF CRFQKCLAVG MVKEVVRTDS LKGRRGRLPS KPKQPPDASP 

       370        380        390        400        410        420 
TNLLTSLIRA HLDSGPNTAK LDYSKFQELV LPRFGKEDAG DVQQFYDLLS GSLDVIRKWA 

       430        440        450        460        470        480 
EKIPGFIELS PGDQDLLLES AFLELFILRL AYRSKPGEGK LIFCSGLVLH RLQCARGFGD 

       490        500        510        520        530        540 
WIDNILAFSR SLHSLGVDVP AFACLSALVL ITDRHGLQDP RRVEELQNRI ASCLKEHMAA 

       550        560        570        580        590 
VAGDPQPASC LSRLLGKLPE LRTLCTQGLQ RIFCLKLEDL VPPPPIVDKI FMDTLSF 

« Hide

References

« Hide 'large scale' references
[1]"Nerve growth factor induces a gene homologous to the glucocorticoid receptor gene."
Milbrandt J.
Neuron 1:183-188(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Placenta.
[3]"The orphan receptors NGFI-B and steroidogenic factor 1 establish monomer binding as a third paradigm of nuclear receptor-DNA interaction."
Wilson T.E., Fahrner T.J., Milbrandt J.
Mol. Cell. Biol. 13:5794-5804(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[4]"Participation of non-zinc finger residues in DNA binding by two nuclear orphan receptors."
Wilson T.E., Paulsen R.E., Padgett K.A., Milbrandt J.
Science 256:107-110(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING REGION.
[5]"The phosphorylation and DNA binding of the DNA-binding domain of the orphan nuclear receptor NGFI-B."
Hirata Y., Kiuchi K., Chen H.-C., Milbrandt J., Guroff G.
J. Biol. Chem. 268:24808-24812(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-340 AND SER-350, MUTAGENESIS.
[6]"DNA-binding mechanism of the monomeric orphan nuclear receptor NGFI-B."
Meinke G., Sigler P.B.
Nat. Struct. Biol. 6:471-477(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 264-351 IN COMPLEX WITH NBRE, METAL-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U17254 mRNA. Translation: AAA56770.1. Different initiation.
BC097313 mRNA. Translation: AAH97313.2. Different initiation.
PIRJQ0623.
RefSeqNP_077364.2. NM_024388.2.
XP_006242418.1. XM_006242356.1.
XP_006242419.1. XM_006242357.1.
XP_006242420.1. XM_006242358.1.
UniGeneRn.10000.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CITX-ray2.70A264-352[»]
1YJEX-ray2.40A354-597[»]
ProteinModelPortalP22829.
SMRP22829. Positions 264-597.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP22829. 1 interaction.
STRING10116.ENSRNOP00000010171.

PTM databases

PhosphoSiteP22829.

Proteomic databases

PaxDbP22829.
PRIDEP22829.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000010171; ENSRNOP00000010171; ENSRNOG00000007607.
GeneID79240.
KEGGrno:79240.

Organism-specific databases

CTD3164.
RGD620029. Nr4a1.

Phylogenomic databases

eggNOGNOG245120.
GeneTreeENSGT00740000115375.
HOGENOMHOG000230925.
HOVERGENHBG052663.
InParanoidP22829.
KOK04465.
OMAYSKFQEL.
OrthoDBEOG75XGKJ.
PhylomeDBP22829.
TreeFamTF315430.

Gene expression databases

GenevestigatorP22829.

Family and domain databases

Gene3D1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProIPR003071. Nuc_orp_HMR_rcpt.
IPR003070. Nuc_orph_rcpt.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSPR01285. HMRNUCRECPTR.
PR01284. NUCLEARECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 1 hit.
PROSITEPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP22829.
NextBio614676.
PROP22829.

Entry information

Entry nameNR4A1_RAT
AccessionPrimary (citable) accession number: P22829
Secondary accession number(s): Q4V8M4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references