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P22829

- NR4A1_RAT

UniProt

P22829 - NR4A1_RAT

Protein

Nuclear receptor subfamily 4 group A member 1

Gene

Nr4a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi263 – 33876Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri266 – 28621NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri302 – 32625NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: InterPro
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
    3. sequence-specific DNA binding Source: Ensembl
    4. steroid hormone receptor activity Source: InterPro
    5. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell migration involved in sprouting angiogenesis Source: Ensembl
    2. cellular response to fibroblast growth factor stimulus Source: Ensembl
    3. cellular response to vascular endothelial growth factor stimulus Source: Ensembl
    4. endothelial cell chemotaxis Source: Ensembl
    5. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
    6. positive regulation of apoptotic process Source: Ensembl
    7. positive regulation of endothelial cell proliferation Source: Ensembl
    8. skeletal muscle cell differentiation Source: Ensembl

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_198729. Constitutive PI3K/AKT Signaling in Cancer.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor subfamily 4 group A member 1
    Alternative name(s):
    NUR77
    Nerve growth factor-induced protein I-B
    Short name:
    NGFI-B
    Orphan nuclear receptor HMR
    Gene namesi
    Name:Nr4a1
    Synonyms:Hmr, Ngfib
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi620029. Nr4a1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: RGD
    3. transcription factor complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi340 – 3401S → A: Loss of phosphorylation. 1 Publication
    Mutagenesisi345 – 3451R → K: Decreased NBRE binding. 1 Publication
    Mutagenesisi348 – 3481L → V: Almost complete loss of NBRE binding. 1 Publication
    Mutagenesisi350 – 3501S → A: Loss of phosphorylation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 597597Nuclear receptor subfamily 4 group A member 1PRO_0000053717Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei340 – 3401Phosphoserine; by PKA1 Publication
    Modified residuei350 – 3501Phosphoserine; by PKA, RPS6KA1 and RPS6KA31 Publication

    Post-translational modificationi

    Phosphorylated at Ser-350 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli By similarity. Phosphorylation of Ser-350 results in decrease in NBRE binding while phosphorylation of Ser-340 has little effect on it.By similarity1 Publication
    Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1 By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP22829.
    PRIDEiP22829.

    PTM databases

    PhosphoSiteiP22829.

    Expressioni

    Tissue specificityi

    Expressed in lung, brain and superior cervical ganglia. High levels are seen in the adrenal tissue.

    Inductioni

    By nerve growth factor and during liver regeneration.

    Gene expression databases

    GenevestigatoriP22829.

    Interactioni

    Subunit structurei

    Interacts with GADD45GIP1. Interacts with STK11 By similarity. Binds DNA as a monomer.By similarity1 Publication

    Protein-protein interaction databases

    IntActiP22829. 1 interaction.
    STRINGi10116.ENSRNOP00000010171.

    Structurei

    Secondary structure

    1
    597
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi267 – 2693
    Beta strandi275 – 2773
    Beta strandi280 – 2823
    Helixi284 – 29613
    Beta strandi303 – 3064
    Turni312 – 3176
    Helixi319 – 32810
    Helixi333 – 3353
    Helixi339 – 3413
    Helixi363 – 37412
    Helixi378 – 3803
    Helixi399 – 42123
    Turni424 – 4285
    Helixi431 – 45323
    Turni456 – 4594
    Beta strandi460 – 4623
    Beta strandi466 – 4705
    Helixi471 – 4788
    Helixi481 – 49515
    Helixi499 – 51012
    Helixi520 – 54021
    Helixi550 – 5556
    Helixi558 – 57821
    Helixi585 – 5928

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CITX-ray2.70A264-352[»]
    1YJEX-ray2.40A354-597[»]
    ProteinModelPortaliP22829.
    SMRiP22829. Positions 264-597.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22829.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni408 – 45851Ligand-bindingSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi80 – 9112Poly-SerAdd
    BLAST
    Compositional biasi182 – 1865Poly-Pro
    Compositional biasi582 – 5854Poly-Pro

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri266 – 28621NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri302 – 32625NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG245120.
    GeneTreeiENSGT00740000115375.
    HOGENOMiHOG000230925.
    HOVERGENiHBG052663.
    InParanoidiP22829.
    KOiK04465.
    OMAiYSKFQEL.
    OrthoDBiEOG75XGKJ.
    PhylomeDBiP22829.
    TreeFamiTF315430.

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProiIPR003071. Nuc_orp_HMR_rcpt.
    IPR003070. Nuc_orph_rcpt.
    IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PRINTSiPR01285. HMRNUCRECPTR.
    PR01284. NUCLEARECPTR.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 1 hit.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22829-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPCIQAQYGT PATSPGPRDH LTGDPLALEF SKPTMDLASP ETAPTAPATL    50
    PSFSTFMDGG YTGEFDTFLY QLPGTAQPCS SASSTSSSSS SATSPASASF 100
    KFEDFQVYGC YPGTLSGPLD ETLSSSGSDY YGSPCSAPSP PTPNFQPSQL 150
    SPWDGSFGHF SPSQTYEGLR VWTEQLPKAS GPPPPPTFFS FSPPTGPSPS 200
    LAQSSLKLFP APATHQLGEG ESYSVPAAFP GLAPTSPNCD TSGILDAPVT 250
    STKARSGSSG GSEGRCAVCG DNASCQHYGV RTCEGCKGFF KRTVQKSAKY 300
    ICLANKDCPV DKRRRNRCQF CRFQKCLAVG MVKEVVRTDS LKGRRGRLPS 350
    KPKQPPDASP TNLLTSLIRA HLDSGPNTAK LDYSKFQELV LPRFGKEDAG 400
    DVQQFYDLLS GSLDVIRKWA EKIPGFIELS PGDQDLLLES AFLELFILRL 450
    AYRSKPGEGK LIFCSGLVLH RLQCARGFGD WIDNILAFSR SLHSLGVDVP 500
    AFACLSALVL ITDRHGLQDP RRVEELQNRI ASCLKEHMAA VAGDPQPASC 550
    LSRLLGKLPE LRTLCTQGLQ RIFCLKLEDL VPPPPIVDKI FMDTLSF 597
    Length:597
    Mass (Da):64,282
    Last modified:October 1, 1996 - v2
    Checksum:i9CFA987112337E53
    GO

    Sequence cautioni

    The sequence AAA56770.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH97313.2 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17254 mRNA. Translation: AAA56770.1. Different initiation.
    BC097313 mRNA. Translation: AAH97313.2. Different initiation.
    PIRiJQ0623.
    RefSeqiNP_077364.2. NM_024388.2.
    XP_006242418.1. XM_006242356.1.
    XP_006242419.1. XM_006242357.1.
    XP_006242420.1. XM_006242358.1.
    UniGeneiRn.10000.

    Genome annotation databases

    EnsembliENSRNOT00000010171; ENSRNOP00000010171; ENSRNOG00000007607.
    GeneIDi79240.
    KEGGirno:79240.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17254 mRNA. Translation: AAA56770.1 . Different initiation.
    BC097313 mRNA. Translation: AAH97313.2 . Different initiation.
    PIRi JQ0623.
    RefSeqi NP_077364.2. NM_024388.2.
    XP_006242418.1. XM_006242356.1.
    XP_006242419.1. XM_006242357.1.
    XP_006242420.1. XM_006242358.1.
    UniGenei Rn.10000.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CIT X-ray 2.70 A 264-352 [» ]
    1YJE X-ray 2.40 A 354-597 [» ]
    ProteinModelPortali P22829.
    SMRi P22829. Positions 264-597.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P22829. 1 interaction.
    STRINGi 10116.ENSRNOP00000010171.

    PTM databases

    PhosphoSitei P22829.

    Proteomic databases

    PaxDbi P22829.
    PRIDEi P22829.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000010171 ; ENSRNOP00000010171 ; ENSRNOG00000007607 .
    GeneIDi 79240.
    KEGGi rno:79240.

    Organism-specific databases

    CTDi 3164.
    RGDi 620029. Nr4a1.

    Phylogenomic databases

    eggNOGi NOG245120.
    GeneTreei ENSGT00740000115375.
    HOGENOMi HOG000230925.
    HOVERGENi HBG052663.
    InParanoidi P22829.
    KOi K04465.
    OMAi YSKFQEL.
    OrthoDBi EOG75XGKJ.
    PhylomeDBi P22829.
    TreeFami TF315430.

    Enzyme and pathway databases

    Reactomei REACT_198729. Constitutive PI3K/AKT Signaling in Cancer.

    Miscellaneous databases

    EvolutionaryTracei P22829.
    NextBioi 614676.
    PROi P22829.

    Gene expression databases

    Genevestigatori P22829.

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProi IPR003071. Nuc_orp_HMR_rcpt.
    IPR003070. Nuc_orph_rcpt.
    IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PRINTSi PR01285. HMRNUCRECPTR.
    PR01284. NUCLEARECPTR.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 1 hit.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nerve growth factor induces a gene homologous to the glucocorticoid receptor gene."
      Milbrandt J.
      Neuron 1:183-188(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.
    3. "The orphan receptors NGFI-B and steroidogenic factor 1 establish monomer binding as a third paradigm of nuclear receptor-DNA interaction."
      Wilson T.E., Fahrner T.J., Milbrandt J.
      Mol. Cell. Biol. 13:5794-5804(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    4. "Participation of non-zinc finger residues in DNA binding by two nuclear orphan receptors."
      Wilson T.E., Paulsen R.E., Padgett K.A., Milbrandt J.
      Science 256:107-110(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING REGION.
    5. "The phosphorylation and DNA binding of the DNA-binding domain of the orphan nuclear receptor NGFI-B."
      Hirata Y., Kiuchi K., Chen H.-C., Milbrandt J., Guroff G.
      J. Biol. Chem. 268:24808-24812(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-340 AND SER-350, MUTAGENESIS.
    6. "DNA-binding mechanism of the monomeric orphan nuclear receptor NGFI-B."
      Meinke G., Sigler P.B.
      Nat. Struct. Biol. 6:471-477(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 264-351 IN COMPLEX WITH NBRE, METAL-BINDING.

    Entry informationi

    Entry nameiNR4A1_RAT
    AccessioniPrimary (citable) accession number: P22829
    Secondary accession number(s): Q4V8M4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3