SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P22829

- NR4A1_RAT

UniProt

P22829 - NR4A1_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Nuclear receptor subfamily 4 group A member 1

Gene
Nr4a1, Hmr, Ngfib
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi263 – 33876Nuclear receptor1 PublicationAdd
BLAST
Zinc fingeri266 – 28621NR C4-typeAdd
BLAST
Zinc fingeri302 – 32625NR C4-typeAdd
BLAST

GO - Molecular functioni

  1. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: InterPro
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
  3. sequence-specific DNA binding Source: Ensembl
  4. steroid hormone receptor activity Source: InterPro
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell migration involved in sprouting angiogenesis Source: Ensembl
  2. cellular response to fibroblast growth factor stimulus Source: Ensembl
  3. cellular response to vascular endothelial growth factor stimulus Source: Ensembl
  4. endothelial cell chemotaxis Source: Ensembl
  5. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
  6. positive regulation of apoptotic process Source: Ensembl
  7. positive regulation of endothelial cell proliferation Source: Ensembl
  8. skeletal muscle cell differentiation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198729. Constitutive PI3K/AKT Signaling in Cancer.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor subfamily 4 group A member 1
Alternative name(s):
NUR77
Nerve growth factor-induced protein I-B
Short name:
NGFI-B
Orphan nuclear receptor HMR
Gene namesi
Name:Nr4a1
Synonyms:Hmr, Ngfib
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi620029. Nr4a1.

Subcellular locationi

Cytoplasm By similarity. Nucleus

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: RGD
  3. transcription factor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi340 – 3401S → A: Loss of phosphorylation.
Mutagenesisi345 – 3451R → K: Decreased NBRE binding.
Mutagenesisi348 – 3481L → V: Almost complete loss of NBRE binding.
Mutagenesisi350 – 3501S → A: Loss of phosphorylation.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 597597Nuclear receptor subfamily 4 group A member 1PRO_0000053717Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei340 – 3401Phosphoserine; by PKA1 Publication
Modified residuei350 – 3501Phosphoserine; by PKA, RPS6KA1 and RPS6KA31 Publication

Post-translational modificationi

Phosphorylated at Ser-350 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli By similarity. Phosphorylation of Ser-350 results in decrease in NBRE binding while phosphorylation of Ser-340 has little effect on it.1 Publication
Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1 By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP22829.
PRIDEiP22829.

PTM databases

PhosphoSiteiP22829.

Expressioni

Tissue specificityi

Expressed in lung, brain and superior cervical ganglia. High levels are seen in the adrenal tissue.

Inductioni

By nerve growth factor and during liver regeneration.

Gene expression databases

GenevestigatoriP22829.

Interactioni

Subunit structurei

Interacts with GADD45GIP1. Interacts with STK11 By similarity. Binds DNA as a monomer.

Protein-protein interaction databases

IntActiP22829. 1 interaction.
STRINGi10116.ENSRNOP00000010171.

Structurei

Secondary structure

1
597
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi267 – 2693
Beta strandi275 – 2773
Beta strandi280 – 2823
Helixi284 – 29613
Beta strandi303 – 3064
Turni312 – 3176
Helixi319 – 32810
Helixi333 – 3353
Helixi339 – 3413
Helixi363 – 37412
Helixi378 – 3803
Helixi399 – 42123
Turni424 – 4285
Helixi431 – 45323
Turni456 – 4594
Beta strandi460 – 4623
Beta strandi466 – 4705
Helixi471 – 4788
Helixi481 – 49515
Helixi499 – 51012
Helixi520 – 54021
Helixi550 – 5556
Helixi558 – 57821
Helixi585 – 5928

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CITX-ray2.70A264-352[»]
1YJEX-ray2.40A354-597[»]
ProteinModelPortaliP22829.
SMRiP22829. Positions 264-597.

Miscellaneous databases

EvolutionaryTraceiP22829.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni408 – 45851Ligand-binding Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi80 – 9112Poly-SerAdd
BLAST
Compositional biasi182 – 1865Poly-Pro
Compositional biasi582 – 5854Poly-Pro

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri266 – 28621NR C4-typeAdd
BLAST
Zinc fingeri302 – 32625NR C4-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG245120.
GeneTreeiENSGT00740000115375.
HOGENOMiHOG000230925.
HOVERGENiHBG052663.
InParanoidiP22829.
KOiK04465.
OMAiYSKFQEL.
OrthoDBiEOG75XGKJ.
PhylomeDBiP22829.
TreeFamiTF315430.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProiIPR003071. Nuc_orp_HMR_rcpt.
IPR003070. Nuc_orph_rcpt.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR01285. HMRNUCRECPTR.
PR01284. NUCLEARECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 1 hit.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22829-1 [UniParc]FASTAAdd to Basket

« Hide

MPCIQAQYGT PATSPGPRDH LTGDPLALEF SKPTMDLASP ETAPTAPATL    50
PSFSTFMDGG YTGEFDTFLY QLPGTAQPCS SASSTSSSSS SATSPASASF 100
KFEDFQVYGC YPGTLSGPLD ETLSSSGSDY YGSPCSAPSP PTPNFQPSQL 150
SPWDGSFGHF SPSQTYEGLR VWTEQLPKAS GPPPPPTFFS FSPPTGPSPS 200
LAQSSLKLFP APATHQLGEG ESYSVPAAFP GLAPTSPNCD TSGILDAPVT 250
STKARSGSSG GSEGRCAVCG DNASCQHYGV RTCEGCKGFF KRTVQKSAKY 300
ICLANKDCPV DKRRRNRCQF CRFQKCLAVG MVKEVVRTDS LKGRRGRLPS 350
KPKQPPDASP TNLLTSLIRA HLDSGPNTAK LDYSKFQELV LPRFGKEDAG 400
DVQQFYDLLS GSLDVIRKWA EKIPGFIELS PGDQDLLLES AFLELFILRL 450
AYRSKPGEGK LIFCSGLVLH RLQCARGFGD WIDNILAFSR SLHSLGVDVP 500
AFACLSALVL ITDRHGLQDP RRVEELQNRI ASCLKEHMAA VAGDPQPASC 550
LSRLLGKLPE LRTLCTQGLQ RIFCLKLEDL VPPPPIVDKI FMDTLSF 597
Length:597
Mass (Da):64,282
Last modified:October 1, 1996 - v2
Checksum:i9CFA987112337E53
GO

Sequence cautioni

The sequence AAA56770.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH97313.2 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17254 mRNA. Translation: AAA56770.1. Different initiation.
BC097313 mRNA. Translation: AAH97313.2. Different initiation.
PIRiJQ0623.
RefSeqiNP_077364.2. NM_024388.2.
XP_006242418.1. XM_006242356.1.
XP_006242419.1. XM_006242357.1.
XP_006242420.1. XM_006242358.1.
UniGeneiRn.10000.

Genome annotation databases

EnsembliENSRNOT00000010171; ENSRNOP00000010171; ENSRNOG00000007607.
GeneIDi79240.
KEGGirno:79240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17254 mRNA. Translation: AAA56770.1 . Different initiation.
BC097313 mRNA. Translation: AAH97313.2 . Different initiation.
PIRi JQ0623.
RefSeqi NP_077364.2. NM_024388.2.
XP_006242418.1. XM_006242356.1.
XP_006242419.1. XM_006242357.1.
XP_006242420.1. XM_006242358.1.
UniGenei Rn.10000.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CIT X-ray 2.70 A 264-352 [» ]
1YJE X-ray 2.40 A 354-597 [» ]
ProteinModelPortali P22829.
SMRi P22829. Positions 264-597.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P22829. 1 interaction.
STRINGi 10116.ENSRNOP00000010171.

PTM databases

PhosphoSitei P22829.

Proteomic databases

PaxDbi P22829.
PRIDEi P22829.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000010171 ; ENSRNOP00000010171 ; ENSRNOG00000007607 .
GeneIDi 79240.
KEGGi rno:79240.

Organism-specific databases

CTDi 3164.
RGDi 620029. Nr4a1.

Phylogenomic databases

eggNOGi NOG245120.
GeneTreei ENSGT00740000115375.
HOGENOMi HOG000230925.
HOVERGENi HBG052663.
InParanoidi P22829.
KOi K04465.
OMAi YSKFQEL.
OrthoDBi EOG75XGKJ.
PhylomeDBi P22829.
TreeFami TF315430.

Enzyme and pathway databases

Reactomei REACT_198729. Constitutive PI3K/AKT Signaling in Cancer.

Miscellaneous databases

EvolutionaryTracei P22829.
NextBioi 614676.
PROi P22829.

Gene expression databases

Genevestigatori P22829.

Family and domain databases

Gene3Di 1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProi IPR003071. Nuc_orp_HMR_rcpt.
IPR003070. Nuc_orph_rcpt.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view ]
Pfami PF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view ]
PRINTSi PR01285. HMRNUCRECPTR.
PR01284. NUCLEARECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTi SM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view ]
SUPFAMi SSF48508. SSF48508. 1 hit.
PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nerve growth factor induces a gene homologous to the glucocorticoid receptor gene."
    Milbrandt J.
    Neuron 1:183-188(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.
  3. "The orphan receptors NGFI-B and steroidogenic factor 1 establish monomer binding as a third paradigm of nuclear receptor-DNA interaction."
    Wilson T.E., Fahrner T.J., Milbrandt J.
    Mol. Cell. Biol. 13:5794-5804(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  4. "Participation of non-zinc finger residues in DNA binding by two nuclear orphan receptors."
    Wilson T.E., Paulsen R.E., Padgett K.A., Milbrandt J.
    Science 256:107-110(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING REGION.
  5. "The phosphorylation and DNA binding of the DNA-binding domain of the orphan nuclear receptor NGFI-B."
    Hirata Y., Kiuchi K., Chen H.-C., Milbrandt J., Guroff G.
    J. Biol. Chem. 268:24808-24812(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-340 AND SER-350, MUTAGENESIS.
  6. "DNA-binding mechanism of the monomeric orphan nuclear receptor NGFI-B."
    Meinke G., Sigler P.B.
    Nat. Struct. Biol. 6:471-477(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 264-351 IN COMPLEX WITH NBRE, METAL-BINDING.

Entry informationi

Entry nameiNR4A1_RAT
AccessioniPrimary (citable) accession number: P22829
Secondary accession number(s): Q4V8M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi