Reviewed, UniProtKB/Swiss-Prot P22823 (DHPH_THEIN)

Names and origin

Protein names

Recommended name:
    Phenylalanine dehydrogenase
      Short name=PheDH
    EC=1.4.1.20

Gene names

Name:

pdh

Organism

Thermoactinomyces intermedius

Taxonomic identifier

2024 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Thermoactinomycetaceae › Thermoactinomyces

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-phenylalanine + H2O + NAD+ = phenylpyruvate + NH3 + NADH.
Pathway	Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-phenylalanine from phenylpyruvate (PDH route): step 1/1.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
biophysicochemical properties	Temperature dependence: Thermostable.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro phenylalanine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Phenylalanine dehydrogenase		PRO_0000182809
Regions
	Nucleotide binding	181 – 187	7	NAD Potential
Sites
	Active site	81	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22823-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 67DFDD87F439D72B	FASTA	366	40,488
	10 20 30 40 50 60 MRDVFEMMDR YGHEQVIFCR HPQTGLKAII ALHNTTAGPA LGGCRMIPYA STDEALEDVL 70 80 90 100 110 120 RLSKGMTYKC SLADVDFGGG KMVIIGDPKK DKSPELFRVI GRFVGGLNGR FYTGTDMGTN 130 140 150 160 170 180 PEDFVHAARE SKSFAGLPKS YGGKGDTSIP TALGVFHGMR ATARFLWGTD QLKGRVVAIQ 190 200 210 220 230 240 GVGKVGERLL QLLVEVGAYC KIADIDSVRC EQLKEKYGDK VQLVDVNRIH KESCDIFSPC 250 260 270 280 290 300 AKGGVVNDDT IDEFRCLAIV GSANNQLVED RHGALLQKRS ICYAPDYLVN AGGLIQVADE 310 320 330 340 350 360 LEGFHEERVL AKTEAIYDMV LDIFHRAKNE NITTCEAADR IVMERLKKLT DIRRILLEDP RNSARR

References

[1]

"Thermostable phenylalanine dehydrogenase of Thermoactinomyces intermedius: cloning, expression, and sequencing of its gene." Takada H., Yoshimura T., Ohshima T., Esaki N., Soda K. J. Biochem. 109:371-376(1991) [PubMed: 1880121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-16. Strain: ATCC 33205 / DSM 43846 / IFO 14230 / JCM 3312 / T-323.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D00631 Genomic DNA. Translation: BAA00524.1.
PIR	JQ0513.
3D structure databases
HSSP	HSSP built from PDB template 1C1D based on UniProtKB Q59771.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.20. 229851.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR016211. Glu/Phe/Leu/Val_DH_bac/arc. [Graphical view]
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000188. Phe_leu_dh. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHPH_THEIN

Accession

Primary (citable) accession number: P22823

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)