Reviewed, UniProtKB/Swiss-Prot P22818 (BIOD_BACSH)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:

bioD

Organism

Bacillus sphaericus

Taxonomic identifier

1421 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Planococcaceae › Lysinibacillus

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subunit structure	Homodimer By similarity. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: InterPro dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000187948
Regions
	Nucleotide binding	8 – 16	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22818-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 20EDA548021457E4	FASTA	234	26,488
	10 20 30 40 50 60 MQHFWVVGTD TDVGKTFVTT LLMRNLQKQG VRVTPYKPVQ TGEVYDGEQA YYFDTAMYEK 70 80 90 100 110 120 YSLQLLDREN LNGYSFKEAA SPHFAAQLEG QQIDTQQLLK QMQLLQQTWD VVICEGAGGL 130 140 150 160 170 180 FVPLDACGET TLLDVIVESK LPVVVVTRTA LGTINHTLLT LEALTTRKIE VLGLVFNGDM 190 200 210 220 230 GSRMEQDNIQ TILQYYTLPY MTIPKLEELS DINEYAITGT SLFERLIRRE TSIN

References

[1]

"Cloning and characterization of the Bacillus sphaericus genes controlling the bioconversion of pimelate into dethiobiotin." Gloeckler R., Ohsawa I., Speck D., Ledoux C., Bernard S., Zinsius M., Villeval D., Kisou T., Kamogawa K., Lemoine Y. Gene 87:63-70(1990) [PubMed: 2110099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10208 / NRS 966 / NCIB 11935 / 1911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29292 Genomic DNA. Translation: AAB02324.1.
PIR	JQ0506.
3D structure databases
SMR	P22818. Positions 4-210.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.3.3. 327.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BACSH

Accession

Primary (citable) accession number: P22818

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)